Source 1primary paper2014PLoS Genetics
Toolkit/PORC promoter
PORC promoter
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The PORC promoter is an Arabidopsis promoter element that functions as an in vivo binding target of the scarecrow-like transcription factor SCL27. Available evidence indicates that SCL27 binds GT cis-elements within this promoter and regulates PORC promoter activity, with DELLA proteins reducing SCL27 promoter binding.
Usefulness & Problems
Why this is useful
This promoter is useful as a native transcriptional target element for studying SCL27-dependent gene regulation in Arabidopsis. It also provides a defined readout for examining how gibberellin-linked DELLA interactions modulate transcription factor occupancy and promoter activity.
Problem solved
The PORC promoter helps address the problem of measuring direct transcriptional regulation by SCL27 in a biologically relevant Arabidopsis context. It also supports analysis of how protein-protein interaction with DELLA factors alters DNA binding at a specific target promoter.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level RNA part used inside a larger architecture that realizes a mechanism.
Mechanisms
protein-protein interaction-dependent modulation of dna bindingsequence-specific dna bindingtranscriptional regulationTechniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: actuator
Implementation is supported primarily in Arabidopsis and in assays that measure SCL27 occupancy or promoter activity. The available evidence specifically mentions chromatin immunoprecipitation and quantitative PCR for in vivo binding, but does not provide construct architecture, minimal promoter boundaries, or delivery details.
The evidence appears to come from a single 2014 study in Arabidopsis, so independent replication is not established here. Practical performance characteristics as a transferable engineered part, such as promoter strength, tissue specificity, dynamic range, or behavior in heterologous systems, are not provided.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
SCL27 binds the promoter region of PORC in vivo.
chromatin immunoprecipitation and quantitative PCR assays showed that SCL27 binds to the promoter region of PORC in vivo.
SCL27 binds the promoter region of PORC in vivo.
chromatin immunoprecipitation and quantitative PCR assays showed that SCL27 binds to the promoter region of PORC in vivo.
SCL27 binds the promoter region of PORC in vivo.
chromatin immunoprecipitation and quantitative PCR assays showed that SCL27 binds to the promoter region of PORC in vivo.
SCL27 binds the promoter region of PORC in vivo.
chromatin immunoprecipitation and quantitative PCR assays showed that SCL27 binds to the promoter region of PORC in vivo.
SCL27 binds the promoter region of PORC in vivo.
chromatin immunoprecipitation and quantitative PCR assays showed that SCL27 binds to the promoter region of PORC in vivo.
SCL27 binds the promoter region of PORC in vivo.
chromatin immunoprecipitation and quantitative PCR assays showed that SCL27 binds to the promoter region of PORC in vivo.
SCL27 binds the promoter region of PORC in vivo.
chromatin immunoprecipitation and quantitative PCR assays showed that SCL27 binds to the promoter region of PORC in vivo.
Interaction with DELLA proteins reduces the binding activity of SCL27 to the PORC promoter.
found that their interaction reduces the binding activity of SCL27 to the PORC promoter
Interaction with DELLA proteins reduces the binding activity of SCL27 to the PORC promoter.
found that their interaction reduces the binding activity of SCL27 to the PORC promoter
Interaction with DELLA proteins reduces the binding activity of SCL27 to the PORC promoter.
found that their interaction reduces the binding activity of SCL27 to the PORC promoter
Interaction with DELLA proteins reduces the binding activity of SCL27 to the PORC promoter.
found that their interaction reduces the binding activity of SCL27 to the PORC promoter
Interaction with DELLA proteins reduces the binding activity of SCL27 to the PORC promoter.
found that their interaction reduces the binding activity of SCL27 to the PORC promoter
Interaction with DELLA proteins reduces the binding activity of SCL27 to the PORC promoter.
found that their interaction reduces the binding activity of SCL27 to the PORC promoter
Interaction with DELLA proteins reduces the binding activity of SCL27 to the PORC promoter.
found that their interaction reduces the binding activity of SCL27 to the PORC promoter
SCL27 regulates PORC promoter activity.
Transient gene expression assays showed that the promoter activity of PORC is indeed regulated by SCL27.
SCL27 regulates PORC promoter activity.
Transient gene expression assays showed that the promoter activity of PORC is indeed regulated by SCL27.
SCL27 regulates PORC promoter activity.
Transient gene expression assays showed that the promoter activity of PORC is indeed regulated by SCL27.
SCL27 regulates PORC promoter activity.
Transient gene expression assays showed that the promoter activity of PORC is indeed regulated by SCL27.
SCL27 regulates PORC promoter activity.
Transient gene expression assays showed that the promoter activity of PORC is indeed regulated by SCL27.
SCL27 regulates PORC promoter activity.
Transient gene expression assays showed that the promoter activity of PORC is indeed regulated by SCL27.
SCL27 regulates PORC promoter activity.
Transient gene expression assays showed that the promoter activity of PORC is indeed regulated by SCL27.
SCL27 directly interacts with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
An electrophoretic mobility shift assay revealed that SCL27 is directly interacted with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
SCL27 directly interacts with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
An electrophoretic mobility shift assay revealed that SCL27 is directly interacted with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
SCL27 directly interacts with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
An electrophoretic mobility shift assay revealed that SCL27 is directly interacted with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
SCL27 directly interacts with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
An electrophoretic mobility shift assay revealed that SCL27 is directly interacted with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
SCL27 directly interacts with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
An electrophoretic mobility shift assay revealed that SCL27 is directly interacted with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
SCL27 directly interacts with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
An electrophoretic mobility shift assay revealed that SCL27 is directly interacted with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
SCL27 directly interacts with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
An electrophoretic mobility shift assay revealed that SCL27 is directly interacted with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug porc-promoter
chromatin immunoprecipitation and quantitative PCR assays showed that SCL27 binds to the promoter region of PORC in vivo.
Source:
dna bindingsupports
SCL27 binds the promoter region of PORC in vivo.
chromatin immunoprecipitation and quantitative PCR assays showed that SCL27 binds to the promoter region of PORC in vivo.
Source:
interaction consequencesupports
Interaction with DELLA proteins reduces the binding activity of SCL27 to the PORC promoter.
found that their interaction reduces the binding activity of SCL27 to the PORC promoter
Source:
promoter regulationsupports
SCL27 regulates PORC promoter activity.
Transient gene expression assays showed that the promoter activity of PORC is indeed regulated by SCL27.
Source:
sequence specific bindingsupports
SCL27 directly interacts with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
An electrophoretic mobility shift assay revealed that SCL27 is directly interacted with G(A/G)(A/T)AA(A/T)GT cis-elements of the PORC promoter.
Source:
Comparisons
Source-backed strengths
Evidence from chromatin immunoprecipitation coupled to quantitative PCR shows that SCL27 binds the PORC promoter in vivo. The literature further supports that SCL27 regulates PORC promoter activity and that GT cis-elements in the promoter are recognized by miR171-targeted scarecrow-like proteins, linking sequence recognition to functional regulation.
Compared with light-controlled crRNA
PORC promoter and light-controlled crRNA address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Compared with P_GAL1-S
PORC promoter and P_GAL1-S address a similar problem space.
Shared frame: shared mechanisms: transcriptional regulation
Strengths here: looks easier to implement in practice.
Compared with transcription activator-like effector nucleases
PORC promoter and transcription activator-like effector nucleases address a similar problem space.
Shared frame: shared mechanisms: sequence-specific dna binding
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.