Source 1review2018Protein Science
Toolkit/post-translational covalent modification for enzyme control
post-translational covalent modification for enzyme control
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Post-translational covalent modification can provide an additional level of enzyme control.
Usefulness & Problems
Why this is useful
This strategy uses post-translational covalent modification to modulate enzyme control beyond sequence-level engineering alone.; adding an additional level of enzyme control; engineering controllable enzyme behavior
Source:
This strategy uses post-translational covalent modification to modulate enzyme control beyond sequence-level engineering alone.
Source:
adding an additional level of enzyme control
Source:
engineering controllable enzyme behavior
Problem solved
It helps create an extra regulatory layer for controlling enzyme structural dynamics and function.; providing extra regulatory control over enzyme function
Source:
It helps create an extra regulatory layer for controlling enzyme structural dynamics and function.
Source:
providing extra regulatory control over enzyme function
Problem links
providing extra regulatory control over enzyme function
LiteratureIt helps create an extra regulatory layer for controlling enzyme structural dynamics and function.
Source:
It helps create an extra regulatory layer for controlling enzyme structural dynamics and function.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
control of conformational changescontrol of enzyme structural dynamicspost-translational covalent modificationTranslation ControlTechniques
Structural CharacterizationTarget processes
translationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: regulator
It requires a modification chemistry or biological modification system compatible with the target enzyme. The abstract does not specify the exact modification platforms.; requires a compatible post-translational covalent modification strategy
The abstract does not establish that it universally enables precise, reversible, or stimulus-specific control in all enzyme systems.; the abstract does not specify which covalent modifications, triggers, or enzyme classes are most suitable
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The ability to engineer and control enzyme structural dynamics can provide new tools for biotechnology and medicine.
Post-translational covalent modification can provide an additional level of enzyme control.
Protein engineering efforts have manipulated enzyme structural dynamics and conformational changes by targeting specific amino acid interactions and by creating chimeric enzymes with new regulatory functions.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug post-translational-covalent-modification-for-enzyme-control
Post-translational covalent modification can provide an additional level of enzyme control.
Source:
application potentialsupports
The ability to engineer and control enzyme structural dynamics can provide new tools for biotechnology and medicine.
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engineering strategy summarysupports
Post-translational covalent modification can provide an additional level of enzyme control.
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Comparisons
Source-stated alternatives
The abstract mentions targeting specific amino acid interactions and creating chimeric enzymes as alternative engineering routes.
Source:
The abstract mentions targeting specific amino acid interactions and creating chimeric enzymes as alternative engineering routes.
Source-backed strengths
adds an additional level of control
Source:
adds an additional level of control
Compared with 4pLRE-cPAOX1
post-translational covalent modification for enzyme control and 4pLRE-cPAOX1 address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control
Strengths here: looks easier to implement in practice.
Compared with blue-light-activated DNA template ON switch
post-translational covalent modification for enzyme control and blue-light-activated DNA template ON switch address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control
Strengths here: looks easier to implement in practice.
Compared with functional electrical stimulation
post-translational covalent modification for enzyme control and functional electrical stimulation address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control
Ranked Citations
- 1.