Source 1review2020Frontiers in Bioengineering and Biotechnology
Toolkit/precise single base editing
precise single base editing
Also known as: single base editing
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Precise single base editing is described in a 2020 review as a CRISPR/Cas-based genome engineering tool within the microbial biotechnology toolbox. It is presented alongside gene activation, gene interference, and orthogonal CRISPR systems as an approach for making precise single-nucleotide changes.
Usefulness & Problems
Why this is useful
The cited review places precise single base editing within CRISPR/Cas applications for microbial genome engineering, multiplexed engineering, and high-throughput screening. It is therefore useful as a precision editing modality in microbial biotechnology contexts where targeted genetic modification is needed.
Source:
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
Source:
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
Problem solved
This tool addresses the need for precise genome engineering at the single-base level within CRISPR/Cas workflows. The supplied evidence does not specify particular target genes, organisms, or editing outcomes beyond its inclusion as a genome engineering approach.
Source:
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
Source:
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
Problem links
Precise single-base editing is a usable genome-engineering tool that could enable targeted modification of microbial functions relevant to methane production or consumption. The link is limited because the supplied evidence is generic and does not connect the method to climate, rumen, or environmental applications.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Techniques
No technique tags yet.
Target processes
editingImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: builder
Implementation is only supported at the level of being a CRISPR/Cas-based genome engineering approach in microbial biotechnology. The evidence does not report construct design, delivery method, host range, guide RNA format, or any cofactor requirements specific to this tool.
The supplied evidence does not identify the editor architecture, Cas protein, deaminase or other catalytic component, editing window, PAM constraints, or by-product profile. No organism-specific validation, efficiency data, off-target analysis, or independent benchmarking for precise single base editing are provided in the extracted text.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
Approval Evidence
1 source1 linked approval claimfirst-pass slug precise-single-base-editing
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
Source:
toolkit scope summarysupports
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
Source:
Comparisons
Source-backed strengths
A key strength supported by the evidence is its positioning as a precise genome engineering modality within the broader CRISPR/Cas toolbox. The review also situates CRISPR/Cas applications in multiplexed engineering, high-throughput screening, and metabolic engineering for production of chemicals and natural compounds, but no tool-specific performance metrics are provided for single base editing.
Compared with caging strategy for crRNA
precise single base editing and caging strategy for crRNA address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Strengths here: looks easier to implement in practice.
Compared with gene editing technology
precise single base editing and gene editing technology address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Compared with multiplexed engineering
precise single base editing and multiplexed engineering address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; shared mechanisms: crispr/cas-mediated genome editing
Ranked Citations
- 1.