Source 1primary paper2026MED
Toolkit/pseudorabies virus (PRV) retrograde transsynaptic tracing
pseudorabies virus (PRV) retrograde transsynaptic tracing
Also known as: PRV, retrograde transsynaptic tracing using pseudorabies virus
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Retrograde transsynaptic tracing using pseudorabies virus (PRV) was employed to identify central amygdala (CeA) involvement in gastric-related neural circuits.
Usefulness & Problems
Why this is useful
This tracing method is used to map gastric-related neural circuits involving the central amygdala. In this study, gastric wall PRV injection revealed an anatomical connection between the stomach and CeA.; identifying neural circuit connectivity related to the stomach and central amygdala
Source:
This tracing method is used to map gastric-related neural circuits involving the central amygdala. In this study, gastric wall PRV injection revealed an anatomical connection between the stomach and CeA.
Source:
identifying neural circuit connectivity related to the stomach and central amygdala
Problem solved
It helps identify whether the CeA is anatomically linked to stomach-related neural circuitry.; provides anatomical tracing evidence for gastric-related neural circuits
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It helps identify whether the CeA is anatomically linked to stomach-related neural circuitry.
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provides anatomical tracing evidence for gastric-related neural circuits
Problem links
provides anatomical tracing evidence for gastric-related neural circuits
LiteratureIt helps identify whether the CeA is anatomically linked to stomach-related neural circuitry.
Source:
It helps identify whether the CeA is anatomically linked to stomach-related neural circuitry.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
retrograde transsynaptic tracingTechniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The abstract indicates that PRV and gastric wall injection are required. Additional procedural details are not provided in the abstract.; requires gastric wall injection of PRV
The abstract does not show that this method alone establishes the precise relay pathway or causal function of the traced circuit.; the abstract does not specify the exact synaptic route or whether the reported connection is monosynaptic versus multisynaptic
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMouseapplication demomouse
Inferred from claim c1 during normalization. Gastric wall PRV tracing revealed an anatomical connection between the stomach and the central amygdala. Derived from claim c1. Quoted text: Gastric wall injection of PRV anatomically revealed a direct connection between the stomach and the CeA.
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Supporting Sources
Ranked Claims
Gastric wall PRV tracing revealed an anatomical connection between the stomach and the central amygdala.
Gastric wall injection of PRV anatomically revealed a direct connection between the stomach and the CeA.
Approval Evidence
1 source1 linked approval claimfirst-pass slug pseudorabies-virus-prv-retrograde-transsynaptic-tracing
Retrograde transsynaptic tracing using pseudorabies virus (PRV) was employed to identify central amygdala (CeA) involvement in gastric-related neural circuits.
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anatomical connectionsupports
Gastric wall PRV tracing revealed an anatomical connection between the stomach and the central amygdala.
Gastric wall injection of PRV anatomically revealed a direct connection between the stomach and the CeA.
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Comparisons
Source-stated alternatives
The abstract contrasts this anatomical tracing approach with functional interrogation methods such as chemogenetic activation, optogenetic activation, and local pharmacological microinjection.
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The abstract contrasts this anatomical tracing approach with functional interrogation methods such as chemogenetic activation, optogenetic activation, and local pharmacological microinjection.
Source-backed strengths
used here to reveal an anatomical connection between the stomach and CeA
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used here to reveal an anatomical connection between the stomach and CeA
The abstract contrasts this anatomical tracing approach with functional interrogation methods such as chemogenetic activation, optogenetic activation, and local pharmacological microinjection.
Shared frame: source-stated alternative in extracted literature
Strengths here: used here to reveal an anatomical connection between the stomach and CeA.
Relative tradeoffs: the abstract does not specify the exact synaptic route or whether the reported connection is monosynaptic versus multisynaptic.
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The abstract contrasts this anatomical tracing approach with functional interrogation methods such as chemogenetic activation, optogenetic activation, and local pharmacological microinjection.
Compared with chemogenetic circuit manipulation
The abstract contrasts this anatomical tracing approach with functional interrogation methods such as chemogenetic activation, optogenetic activation, and local pharmacological microinjection.
Shared frame: source-stated alternative in extracted literature
Strengths here: used here to reveal an anatomical connection between the stomach and CeA.
Relative tradeoffs: the abstract does not specify the exact synaptic route or whether the reported connection is monosynaptic versus multisynaptic.
Source:
The abstract contrasts this anatomical tracing approach with functional interrogation methods such as chemogenetic activation, optogenetic activation, and local pharmacological microinjection.
Compared with optogenetic
The abstract contrasts this anatomical tracing approach with functional interrogation methods such as chemogenetic activation, optogenetic activation, and local pharmacological microinjection.
Shared frame: source-stated alternative in extracted literature
Strengths here: used here to reveal an anatomical connection between the stomach and CeA.
Relative tradeoffs: the abstract does not specify the exact synaptic route or whether the reported connection is monosynaptic versus multisynaptic.
Source:
The abstract contrasts this anatomical tracing approach with functional interrogation methods such as chemogenetic activation, optogenetic activation, and local pharmacological microinjection.
Ranked Citations
- 1.