Source 1review2016Photochemistry and Photobiology
Toolkit/pulsed laser-induced transient grating technique
pulsed laser-induced transient grating technique
Also known as: TG method, time-resolved diffusion technique, transient grating technique
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The pulsed laser-induced transient grating technique is a time-resolved optical assay method used to detect conformational changes in proteins in solution after photoexcitation. In the cited application, it was used to monitor time-domain conformational changes in oat phytochrome A following excitation of the red-absorbing Pr form.
Usefulness & Problems
Why this is useful
This method is useful for tracking photoinduced protein conformational dynamics in solution with time resolution. The supplied evidence specifically supports its use for observing conformational changes in oat phytochrome A after light activation.
Problem solved
It addresses the problem of detecting time-dependent conformational changes in a photoresponsive protein in solution after photoexcitation. The cited study applies it to oat phytochrome A in the Pr state.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
Conformational Uncaginglight-triggered conformational change detectionlight-triggered conformational change detectiontransient grating-based time-resolved optical readouttransient grating-based time-resolved optical readoutTechniques
Functional AssayTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
application domain: light-responsive protein dynamicscofactor dependency: cofactor requirement unknownimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementmeasurement readout: diffusion coefficientmeasurement readout: time-dependent diffusion coefficientoperating role: sensorsolution assay: Truetime domain: True
The cited application used pulsed laser-induced transient grating measurements on oat phytochrome A in solution after photoexcitation of the Pr form. Beyond this light-triggered solution assay context, the provided evidence does not specify construct requirements, cofactors, instrumentation parameters, or expression and delivery considerations.
The supplied evidence covers only a single application in oat phytochrome A and does not establish generality across proteins or assay settings. No quantitative performance metrics, wavelength details, sensitivity limits, or comparative benchmarking are provided in the evidence here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2006Biophysical Journal
Ranked Claims
Cryptochrome exhibits a considerable diffusion change with a time constant of 400 milliseconds, indicating altered protein-solvent interaction due to conformational change.
cryptochrome diffusion-change time constant 400 milliseconds
In the reviewed comparison, conformational change in (6-4) photolyase was not detected by monitoring the diffusion coefficient after photoexcitation, but repaired DNA dissociated from photolyase with a time constant of 50 microseconds, consistent with a minor conformational change.
DNA dissociation time constant 50 microseconds
The pulsed laser-induced transient grating technique can detect time-dependent biomolecular interactions, including transient dissociation reactions in solution, in real time.
Pulsed laser-induced transient grating was used to study time-domain conformational changes in oat phytochrome A in solution after photoexcitation of the Pr form.
Conformational changes in oat phytochrome A (phy) in solution after photoexcitation of the red-absorbing form (Pr) were studied in time-domain by the pulsed laser-induced transient grating technique.
Pulsed laser-induced transient grating was used to study time-domain conformational changes in oat phytochrome A in solution after photoexcitation of the Pr form.
Conformational changes in oat phytochrome A (phy) in solution after photoexcitation of the red-absorbing form (Pr) were studied in time-domain by the pulsed laser-induced transient grating technique.
Pulsed laser-induced transient grating was used to study time-domain conformational changes in oat phytochrome A in solution after photoexcitation of the Pr form.
Conformational changes in oat phytochrome A (phy) in solution after photoexcitation of the red-absorbing form (Pr) were studied in time-domain by the pulsed laser-induced transient grating technique.
Pulsed laser-induced transient grating was used to study time-domain conformational changes in oat phytochrome A in solution after photoexcitation of the Pr form.
Conformational changes in oat phytochrome A (phy) in solution after photoexcitation of the red-absorbing form (Pr) were studied in time-domain by the pulsed laser-induced transient grating technique.
Pulsed laser-induced transient grating was used to study time-domain conformational changes in oat phytochrome A in solution after photoexcitation of the Pr form.
Conformational changes in oat phytochrome A (phy) in solution after photoexcitation of the red-absorbing form (Pr) were studied in time-domain by the pulsed laser-induced transient grating technique.
Approval Evidence
2 sources4 linked approval claimsfirst-pass slug pulsed-laser-induced-transient-grating-technique
we compare the reaction dynamics of these systems by monitoring the reaction kinetics of conformational change and intermolecular interaction change based on time-dependent diffusion coefficient measurements obtained by using the pulsed laser-induced transient grating technique. Using this method, time-dependent biomolecular interactions, such as transient dissociation reactions in solution, have been successfully detected in real time.
Source:
Conformational changes in oat phytochrome A (phy) in solution after photoexcitation of the red-absorbing form (Pr) were studied in time-domain by the pulsed laser-induced transient grating technique.
Source:
comparative mechanistic summarysupports
Cryptochrome exhibits a considerable diffusion change with a time constant of 400 milliseconds, indicating altered protein-solvent interaction due to conformational change.
Source:
comparative mechanistic summarysupports
In the reviewed comparison, conformational change in (6-4) photolyase was not detected by monitoring the diffusion coefficient after photoexcitation, but repaired DNA dissociated from photolyase with a time constant of 50 microseconds, consistent with a minor conformational change.
Source:
method capabilitysupports
The pulsed laser-induced transient grating technique can detect time-dependent biomolecular interactions, including transient dissociation reactions in solution, in real time.
Source:
method applicationsupports
Pulsed laser-induced transient grating was used to study time-domain conformational changes in oat phytochrome A in solution after photoexcitation of the Pr form.
Conformational changes in oat phytochrome A (phy) in solution after photoexcitation of the red-absorbing form (Pr) were studied in time-domain by the pulsed laser-induced transient grating technique.
Source:
Comparisons
Source-backed strengths
The method provides a time-resolved optical readout of conformational changes after photoexcitation in solution. The available evidence demonstrates successful application to oat phytochrome A, indicating suitability for monitoring light-triggered structural dynamics in this context.
Ranked Citations
- 1.
- 2.