Source 1review2022Annual Review of Analytical Chemistry
Toolkit/transient absorption super-resolution imaging
transient absorption super-resolution imaging
Also known as: transient absorption
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
These label-free techniques span a variety of different approaches, including structured illumination, transient absorption, infrared absorption, and coherent Raman spectroscopies.
Usefulness & Problems
Why this is useful
Transient absorption is listed as one of the label-free approaches used for super-resolution imaging. It is part of the review's survey of nonfluorescent imaging strategies.; label-free super-resolution imaging
Source:
Transient absorption is listed as one of the label-free approaches used for super-resolution imaging. It is part of the review's survey of nonfluorescent imaging strategies.
Source:
label-free super-resolution imaging
Problem solved
It contributes to imaging nanoscale heterogeneity without requiring fluorescent labels.; super-resolution imaging without fluorescent labels
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It contributes to imaging nanoscale heterogeneity without requiring fluorescent labels.
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super-resolution imaging without fluorescent labels
Problem links
This item is explicitly described as part of a set of label-free imaging techniques, which could reduce or eliminate the need to deliver physical probes into living cells. That aligns with the gap's emphasis on noninvasive imaging alternatives.
super-resolution imaging without fluorescent labels
LiteratureIt contributes to imaging nanoscale heterogeneity without requiring fluorescent labels.
Source:
It contributes to imaging nanoscale heterogeneity without requiring fluorescent labels.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
transient absorptionTechniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
Many label-free super-resolution methods draw inspiration from fluorescence-based STED, PALM, and STORM.
The abstract does not specify comparative strengths, weaknesses, or whether it matches fluorescence-based resolution.; the abstract does not provide explicit resolution, sample, or hardware details
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Super-resolution fluorescence methods can break the optical diffraction limit but require fluorescent labeling.
Many label-free super-resolution methods draw inspiration from fluorescence-based STED, PALM, and STORM.
Label-free super-resolution techniques include structured illumination, transient absorption, infrared absorption, and coherent Raman spectroscopies.
Approval Evidence
1 source1 linked approval claimfirst-pass slug transient-absorption-super-resolution-imaging
These label-free techniques span a variety of different approaches, including structured illumination, transient absorption, infrared absorption, and coherent Raman spectroscopies.
Source:
field summarysupports
Label-free super-resolution techniques include structured illumination, transient absorption, infrared absorption, and coherent Raman spectroscopies.
Source:
Comparisons
Source-stated alternatives
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Source:
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Source-backed strengths
included as a label-free super-resolution approach
Source:
included as a label-free super-resolution approach
Compared with infrared absorption super-resolution imaging
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: included as a label-free super-resolution approach.
Relative tradeoffs: the abstract does not provide explicit resolution, sample, or hardware details.
Source:
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Compared with PALM
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: included as a label-free super-resolution approach.
Relative tradeoffs: the abstract does not provide explicit resolution, sample, or hardware details.
Source:
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Compared with photoactivated localization microscopy
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: included as a label-free super-resolution approach.
Relative tradeoffs: the abstract does not provide explicit resolution, sample, or hardware details.
Source:
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Compared with photo-activation localization microscopy
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: included as a label-free super-resolution approach.
Relative tradeoffs: the abstract does not provide explicit resolution, sample, or hardware details.
Source:
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Compared with photoactivation localization microscopy
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: included as a label-free super-resolution approach.
Relative tradeoffs: the abstract does not provide explicit resolution, sample, or hardware details.
Source:
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Compared with STED
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: included as a label-free super-resolution approach.
Relative tradeoffs: the abstract does not provide explicit resolution, sample, or hardware details.
Source:
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Compared with STED microscopy
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: included as a label-free super-resolution approach.
Relative tradeoffs: the abstract does not provide explicit resolution, sample, or hardware details.
Source:
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Compared with stimulated emission depletion microscopy
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: included as a label-free super-resolution approach.
Relative tradeoffs: the abstract does not provide explicit resolution, sample, or hardware details.
Source:
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Compared with stochastic optical reconstruction microscopy
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: included as a label-free super-resolution approach.
Relative tradeoffs: the abstract does not provide explicit resolution, sample, or hardware details.
Source:
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Compared with STORM
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: included as a label-free super-resolution approach.
Relative tradeoffs: the abstract does not provide explicit resolution, sample, or hardware details.
Source:
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Compared with structured illumination
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: included as a label-free super-resolution approach.
Relative tradeoffs: the abstract does not provide explicit resolution, sample, or hardware details.
Source:
The review abstract places it alongside structured illumination, infrared absorption, coherent Raman methods, and fluorescence-based STED, PALM, and STORM.
Ranked Citations
- 1.