Source 1primary paper2022MED
Toolkit/RCaMP
RCaMP
Also known as: red calmodulin fusion protein
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Development of mice expressing optogenetic sensors (green calmodulin fusion protein (GCaMP), red calmodulin fusion protein (RCaMP)) has allowed visualization of Ca2+ signalling behaviours in a cell specific manner.
Usefulness & Problems
Why this is useful
RCaMP is described as a red calmodulin fusion protein used as an optogenetic sensor in mice for cell-specific visualization of Ca2+ signalling behaviours.; cell-specific visualization of Ca2+ signalling behaviours in the GI tract
Source:
RCaMP is described as a red calmodulin fusion protein used as an optogenetic sensor in mice for cell-specific visualization of Ca2+ signalling behaviours.
Source:
cell-specific visualization of Ca2+ signalling behaviours in the GI tract
Problem solved
It is presented as part of the shift from non-specific Ca2+ dye loading to cell-specific optical readout in GI tissues.; supports cell-specific Ca2+ imaging instead of indiscriminate dye loading
Source:
It is presented as part of the shift from non-specific Ca2+ dye loading to cell-specific optical readout in GI tissues.
Source:
supports cell-specific Ca2+ imaging instead of indiscriminate dye loading
Problem links
supports cell-specific Ca2+ imaging instead of indiscriminate dye loading
LiteratureIt is presented as part of the shift from non-specific Ca2+ dye loading to cell-specific optical readout in GI tissues.
Source:
It is presented as part of the shift from non-specific Ca2+ dye loading to cell-specific optical readout in GI tissues.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
calcium-dependent fluorescent sensingTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The abstract supports use in mice expressing RCaMP. No additional implementation requirements are given.; requires mice expressing the optogenetic sensor
The abstract does not provide evidence that RCaMP was used for pathway manipulation or neural circuit actuation.; abstract does not describe specific GI applications or performance details for RCaMP
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Animals expressing channelrhodopsin in specific neuronal populations have been used to map neural circuitry and examine post junctional neural effects on GI motility.
GCaMP-expressing animals have been used to characterize Ca2+ signalling behaviours of distinct classes of interstitial cells of Cajal and smooth muscle cells throughout the GI musculature.
Mice expressing GCaMP or RCaMP allow cell-specific visualization of Ca2+ signalling behaviours in the gastrointestinal tract.
Mice expressing channelrhodopsins or halorhodopsins allow light-based manipulation of specific signalling pathways.
Approval Evidence
1 source1 linked approval claimfirst-pass slug rcamp
Development of mice expressing optogenetic sensors (green calmodulin fusion protein (GCaMP), red calmodulin fusion protein (RCaMP)) has allowed visualization of Ca2+ signalling behaviours in a cell specific manner.
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enables measurementsupports
Mice expressing GCaMP or RCaMP allow cell-specific visualization of Ca2+ signalling behaviours in the gastrointestinal tract.
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Comparisons
Source-stated alternatives
The abstract mentions GCaMP as another optogenetic sensor and contrasts both with indiscriminate Ca2+ dye loading.
Source:
The abstract mentions GCaMP as another optogenetic sensor and contrasts both with indiscriminate Ca2+ dye loading.
Source-backed strengths
presented as an optogenetic sensor enabling cell-specific visualization
Source:
presented as an optogenetic sensor enabling cell-specific visualization
Compared with GCaMP
The abstract mentions GCaMP as another optogenetic sensor and contrasts both with indiscriminate Ca2+ dye loading.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as an optogenetic sensor enabling cell-specific visualization.
Relative tradeoffs: abstract does not describe specific GI applications or performance details for RCaMP.
Source:
The abstract mentions GCaMP as another optogenetic sensor and contrasts both with indiscriminate Ca2+ dye loading.
Compared with GCaMP calcium imaging
The abstract mentions GCaMP as another optogenetic sensor and contrasts both with indiscriminate Ca2+ dye loading.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as an optogenetic sensor enabling cell-specific visualization.
Relative tradeoffs: abstract does not describe specific GI applications or performance details for RCaMP.
Source:
The abstract mentions GCaMP as another optogenetic sensor and contrasts both with indiscriminate Ca2+ dye loading.
Compared with optogenetic
The abstract mentions GCaMP as another optogenetic sensor and contrasts both with indiscriminate Ca2+ dye loading.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as an optogenetic sensor enabling cell-specific visualization.
Relative tradeoffs: abstract does not describe specific GI applications or performance details for RCaMP.
Source:
The abstract mentions GCaMP as another optogenetic sensor and contrasts both with indiscriminate Ca2+ dye loading.
Ranked Citations
- 1.