Source 1review1992Cytometry
Toolkit/Rhodamine 123 mitochondrial transmembrane potential assay
Rhodamine 123 mitochondrial transmembrane potential assay
Also known as: rhodamine 123 retention assay
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Mitochondrial transmembrane potential, assayed by retention of rhodamine 123 was preserved in apoptotic but not necrotic cells.
Usefulness & Problems
Why this is useful
This assay uses rhodamine 123 retention as a flow-cytometric readout of mitochondrial transmembrane potential.; assaying mitochondrial transmembrane potential; distinguishing apoptotic from necrotic cells
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This assay uses rhodamine 123 retention as a flow-cytometric readout of mitochondrial transmembrane potential.
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assaying mitochondrial transmembrane potential
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distinguishing apoptotic from necrotic cells
Problem solved
It helps distinguish apoptotic from necrotic cells by monitoring whether mitochondrial transmembrane potential is preserved.; provides an organelle-function readout that differs between apoptotic and necrotic cells
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It helps distinguish apoptotic from necrotic cells by monitoring whether mitochondrial transmembrane potential is preserved.
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provides an organelle-function readout that differs between apoptotic and necrotic cells
Problem links
provides an organelle-function readout that differs between apoptotic and necrotic cells
LiteratureIt helps distinguish apoptotic from necrotic cells by monitoring whether mitochondrial transmembrane potential is preserved.
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It helps distinguish apoptotic from necrotic cells by monitoring whether mitochondrial transmembrane potential is preserved.
Published Workflows
Objective: Differentiate apoptosis from necrosis and identify early apoptotic changes and cell-cycle phase specificity using multiparameter flow-cytometric readouts.
Why it works: The review describes combining orthogonal cellular features so that apoptosis and necrosis can be separated by differences in DNA behavior, membrane integrity, organelle function, and scatter properties rather than relying on a single marker.
endonuclease-associated low molecular weight DNA extractionloss or preservation of plasma membrane integritypreservation or loss of mitochondrial transmembrane potentialpreservation or loss of ATP-dependent lysosomal proton pumpingDNA strand-break formationflow cytometrymultiparameter fluorescent stainingbivariate analysis
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
It requires rhodamine 123 and a fluorescence-capable flow cytometer.; requires rhodamine 123 staining; depends on flow-cytometric retention measurement
The abstract does not indicate that it alone resolves early versus late apoptotic stages or cell-cycle specificity.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Acridine orange staining at low pH provides a sensitive and early assay to discriminate live, apoptotic, and necrotic cells and to evaluate cell-cycle phase specificity.
Propidium iodide exclusion combined with Hoechst 33342 staining is described as an excellent probe for distinguishing live, necrotic, early-apoptotic, and late-apoptotic cells.
Rhodamine 123 retention indicates that mitochondrial transmembrane potential is preserved in apoptotic but not necrotic cells.
Supravital acridine orange uptake indicates that ATP-dependent lysosomal proton pump function is preserved in apoptotic but not necrotic cells.
Approval Evidence
1 source1 linked approval claimfirst-pass slug rhodamine-123-mitochondrial-transmembrane-potential-assay
Mitochondrial transmembrane potential, assayed by retention of rhodamine 123 was preserved in apoptotic but not necrotic cells.
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review summarysupports
Rhodamine 123 retention indicates that mitochondrial transmembrane potential is preserved in apoptotic but not necrotic cells.
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Comparisons
Source-stated alternatives
The review contrasts this with PI/Hoechst membrane-integrity probing, acridine orange assays, DNA content measurements, and nick translation for DNA strand breaks.
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The review contrasts this with PI/Hoechst membrane-integrity probing, acridine orange assays, DNA content measurements, and nick translation for DNA strand breaks.
Source-backed strengths
captures preservation of mitochondrial transmembrane potential in apoptotic but not necrotic cells
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captures preservation of mitochondrial transmembrane potential in apoptotic but not necrotic cells
Compared with assays
The review contrasts this with PI/Hoechst membrane-integrity probing, acridine orange assays, DNA content measurements, and nick translation for DNA strand breaks.
Shared frame: source-stated alternative in extracted literature
Strengths here: captures preservation of mitochondrial transmembrane potential in apoptotic but not necrotic cells.
Source:
The review contrasts this with PI/Hoechst membrane-integrity probing, acridine orange assays, DNA content measurements, and nick translation for DNA strand breaks.
Ranked Citations
- 1.