Source 1review2005Annals of Botany
Toolkit/SCFTIR1 E3 ubiquitin ligase complex
SCFTIR1 E3 ubiquitin ligase complex
Also known as: SCFTIR1
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Auxin signalling is mediated, at least in large part, by an SCFTIR1 E3 ubiquitin ligase complex that accelerates Aux/IAA repressor degradation in response to IAA, thereby altering gene expression.
Usefulness & Problems
Why this is useful
This complex is described as a major mediator of auxin signaling that accelerates degradation of Aux/IAA repressors in response to IAA. The resulting change in repressor abundance alters gene expression.; auxin-responsive protein degradation; auxin signaling studies; linking small-molecule auxin input to transcriptional output
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This complex is described as a major mediator of auxin signaling that accelerates degradation of Aux/IAA repressors in response to IAA. The resulting change in repressor abundance alters gene expression.
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auxin-responsive protein degradation
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auxin signaling studies
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linking small-molecule auxin input to transcriptional output
Problem solved
It provides a mechanistic link between auxin presence and downstream transcriptional response through targeted proteolysis.; mediates auxin-dependent degradation of Aux/IAA repressors; provides a mechanism for converting IAA perception into altered gene expression
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It provides a mechanistic link between auxin presence and downstream transcriptional response through targeted proteolysis.
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mediates auxin-dependent degradation of Aux/IAA repressors
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provides a mechanism for converting IAA perception into altered gene expression
Problem links
mediates auxin-dependent degradation of Aux/IAA repressors
LiteratureIt provides a mechanistic link between auxin presence and downstream transcriptional response through targeted proteolysis.
Source:
It provides a mechanistic link between auxin presence and downstream transcriptional response through targeted proteolysis.
provides a mechanism for converting IAA perception into altered gene expression
LiteratureIt provides a mechanistic link between auxin presence and downstream transcriptional response through targeted proteolysis.
Source:
It provides a mechanistic link between auxin presence and downstream transcriptional response through targeted proteolysis.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
degradationsignalingImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: regulator
Its action is described in the context of IAA and Aux/IAA repressors, so those signaling components are prerequisites in the reviewed mechanism.; requires auxin/IAA-responsive context; depends on Aux/IAA repressor substrates
The abstract does not claim that SCFTIR1 alone explains all auxin signaling or broader auxin metabolism and transport.; the review states signaling is mediated at least in large part by this complex, implying the mechanism is not fully exhaustive
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Auxin signaling is mediated at least in large part by an SCFTIR1 E3 ubiquitin ligase complex that accelerates Aux/IAA repressor degradation in response to IAA, thereby altering gene expression.
Approval Evidence
1 source1 linked approval claimfirst-pass slug scftir1-e3-ubiquitin-ligase-complex
Auxin signalling is mediated, at least in large part, by an SCFTIR1 E3 ubiquitin ligase complex that accelerates Aux/IAA repressor degradation in response to IAA, thereby altering gene expression.
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review summarysupports
Auxin signaling is mediated at least in large part by an SCFTIR1 E3 ubiquitin ligase complex that accelerates Aux/IAA repressor degradation in response to IAA, thereby altering gene expression.
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Comparisons
Source-stated alternatives
The review also discusses auxin transport, biosynthesis, conjugation, and hormone interactions as other layers controlling auxin response.
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The review also discusses auxin transport, biosynthesis, conjugation, and hormone interactions as other layers controlling auxin response.
Source-backed strengths
presented as a major mediator of auxin signaling; connects hormone response to regulated proteolysis
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presented as a major mediator of auxin signaling
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connects hormone response to regulated proteolysis
Compared with CAR-NK cells
SCFTIR1 E3 ubiquitin ligase complex and CAR-NK cells address a similar problem space because they share degradation, signaling.
Shared frame: same top-level item type; shared target processes: degradation, signaling; shared mechanisms: degradation
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
SCFTIR1 E3 ubiquitin ligase complex and Jalpha helix point mutations disrupting LOV-domain interaction address a similar problem space because they share degradation, signaling.
Shared frame: same top-level item type; shared target processes: degradation, signaling; shared mechanisms: degradation
Strengths here: looks easier to implement in practice.
Compared with PCB synthesis expression vector
SCFTIR1 E3 ubiquitin ligase complex and PCB synthesis expression vector address a similar problem space because they share degradation, signaling.
Shared frame: same top-level item type; shared target processes: degradation, signaling; shared mechanisms: degradation
Strengths here: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Ranked Citations
- 1.