Source 1review2022Frontiers in Genome Editing
Toolkit/sequencing-based solutions
sequencing-based solutions
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Sequencing-based solutions are proposed assay methods for detecting large-scale CRISPR-associated genomic alterations. In the cited review, they are positioned as potential approaches to identify rare events such as translocations, inversions, deletions, and chromothripsis that can be missed by current workflows.
Usefulness & Problems
Why this is useful
These methods are useful because they may extend CRISPR safety assessment beyond small on-target or off-target sequence changes to include large-scale genomic damage. The cited review specifically highlights their potential to detect such events even when they occur at low frequencies.
Problem solved
The specific problem is the difficulty of detecting large-scale aberrations generated in CRISPR editing workflows, including translocations, inversions, deletions, and chromothripsis. The review describes this as a major unmet need in current detection workflows.
Problem links
Sequencing-based assays could plausibly help track emerging microbial variants or rare harmful changes, which is relevant to keeping defenses updated against fast-evolving threats. The supplied summary supports detection at low frequencies, making it more actionable than generic background concepts.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Target processes
editinglocalizationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The cited evidence places these methods in the context of CRISPR editing assessment, including ex vivo clinical workflows involving stem cell isolation, modification, and re-transplantation. However, the source text does not specify sequencing platform, library design, sample preparation, or analysis pipeline requirements.
The evidence provided is limited to a review-level statement that these are potential solutions, without naming a specific assay format or reporting performance metrics. No direct validation data, implementation details, or independent replication are supplied in the cited evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Many current clinical CRISPR trials use an ex vivo workflow involving stem cell isolation, modification, and re-transplantation.
many of the current clinical trials using CRISPR involve ex vivo isolation of a patient's own stem cells, modification, and re-transplantation
Many current clinical CRISPR trials use an ex vivo workflow involving stem cell isolation, modification, and re-transplantation.
many of the current clinical trials using CRISPR involve ex vivo isolation of a patient's own stem cells, modification, and re-transplantation
Many current clinical CRISPR trials use an ex vivo workflow involving stem cell isolation, modification, and re-transplantation.
many of the current clinical trials using CRISPR involve ex vivo isolation of a patient's own stem cells, modification, and re-transplantation
Many current clinical CRISPR trials use an ex vivo workflow involving stem cell isolation, modification, and re-transplantation.
many of the current clinical trials using CRISPR involve ex vivo isolation of a patient's own stem cells, modification, and re-transplantation
Many current clinical CRISPR trials use an ex vivo workflow involving stem cell isolation, modification, and re-transplantation.
many of the current clinical trials using CRISPR involve ex vivo isolation of a patient's own stem cells, modification, and re-transplantation
Many current clinical CRISPR trials use an ex vivo workflow involving stem cell isolation, modification, and re-transplantation.
many of the current clinical trials using CRISPR involve ex vivo isolation of a patient's own stem cells, modification, and re-transplantation
Many current clinical CRISPR trials use an ex vivo workflow involving stem cell isolation, modification, and re-transplantation.
many of the current clinical trials using CRISPR involve ex vivo isolation of a patient's own stem cells, modification, and re-transplantation
Many current clinical CRISPR trials use an ex vivo workflow involving stem cell isolation, modification, and re-transplantation.
many of the current clinical trials using CRISPR involve ex vivo isolation of a patient's own stem cells, modification, and re-transplantation
Many current clinical CRISPR trials use an ex vivo workflow involving stem cell isolation, modification, and re-transplantation.
many of the current clinical trials using CRISPR involve ex vivo isolation of a patient's own stem cells, modification, and re-transplantation
Many current clinical CRISPR trials use an ex vivo workflow involving stem cell isolation, modification, and re-transplantation.
many of the current clinical trials using CRISPR involve ex vivo isolation of a patient's own stem cells, modification, and re-transplantation
Approval Evidence
1 source2 linked approval claimsfirst-pass slug sequencing-based-solutions
In this review we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence.
Source:
limitation summarysupports
Large-scale aberrations such as translocations, inversions, deletions, and chromothripsis are more difficult to detect using current workflows, indicating a major unmet need.
However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field.
Source:
potential solutionsupports
Sequencing-based solutions may be able to detect large-scale CRISPR-associated genomic effects even at low frequencies of occurrence.
we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence
Source:
Comparisons
Source-backed strengths
A key proposed strength is sensitivity to large-scale CRISPR-associated genomic effects that are difficult to capture with existing workflows. The review further suggests that sequencing-based approaches may detect these events even at low frequencies of occurrence.
Compared with CRY2-DNMT3A-CD fusion
sequencing-based solutions and CRY2-DNMT3A-CD fusion address a similar problem space because they share editing, localization.
Shared frame: shared target processes: editing, localization
Strengths here: looks easier to implement in practice.
sequencing-based solutions and lateral flow assay strip test combined with CRISPR/Cas12a address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Strengths here: looks easier to implement in practice.
Compared with whole genome screening of gene knockout mutants
sequencing-based solutions and whole genome screening of gene knockout mutants address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Ranked Citations
- 1.