Source 1primary paper2024Analytical Chemistry
Toolkit/lateral flow assay strip test combined with CRISPR/Cas12a
lateral flow assay strip test combined with CRISPR/Cas12a
Also known as: lateral flow assay strip test with the CRISPR/Cas12a system
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
This assay method combines a lateral flow assay strip test with a CRISPR/Cas12a sensing system to visualize nucleic acid cleavage signals. In the cited 2024 Analytical Chemistry study, it was presented within a photoactivatable CRISPR/Cas12a platform for DNA and RNA detection with point-of-care diagnostic potential.
Usefulness & Problems
Why this is useful
The method is useful because it converts CRISPR/Cas12a nucleic acid cleavage activity into a strip-based visual readout, supporting rapid and potentially instant testing. The associated photoactivatable design was reported to provide high spatiotemporal control of nucleic acid sensing and to reduce contamination risk from premature signal leakage during storage.
Source:
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
Problem solved
This tool addresses the problem of making CRISPR/Cas12a nucleic acid sensing visually accessible in a lateral flow format suitable for point-of-care use. In the cited study, it also addressed the need for temporary control over sensing activation to limit premature activity and contamination risk before use.
Problem links
Need controllable genome or transcript editing
DerivedThis assay method combines a lateral flow assay strip test with a CRISPR/Cas12a sensing system to visualize nucleic acid cleavage signals. In the cited 2024 Analytical Chemistry study, it was presented within a photoactivatable CRISPR/Cas12a platform for DNA and RNA detection with point-of-care diagnostic potential.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
editingImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenoperating role: sensorswitch architecture: cleavageswitch architecture: multi component
The assay requires integration of a lateral flow strip readout with a CRISPR/Cas12a sensing system. The cited study further indicates that the overall platform incorporated photoactivation for controlled DNA and RNA sensing, but the supplied evidence does not specify construct design, illumination wavelength, reagent composition, or sample preparation details.
The supplied evidence does not provide quantitative performance metrics such as sensitivity, specificity, limit of detection, assay time, or comparative benchmarking against other lateral flow CRISPR assays. Evidence is drawn from a single study, and the lateral flow component is only explicitly supported for visualization of cleavage signals rather than broad clinical validation.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
The study integrated photoactivation with CRISPR/Cas12a for DNA and RNA detection to provide high spatiotemporal control of nucleic acid sensing.
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
The study integrated photoactivation with CRISPR/Cas12a for DNA and RNA detection to provide high spatiotemporal control of nucleic acid sensing.
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
The study integrated photoactivation with CRISPR/Cas12a for DNA and RNA detection to provide high spatiotemporal control of nucleic acid sensing.
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
The study integrated photoactivation with CRISPR/Cas12a for DNA and RNA detection to provide high spatiotemporal control of nucleic acid sensing.
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
The study integrated photoactivation with CRISPR/Cas12a for DNA and RNA detection to provide high spatiotemporal control of nucleic acid sensing.
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
The study integrated photoactivation with CRISPR/Cas12a for DNA and RNA detection to provide high spatiotemporal control of nucleic acid sensing.
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
The study integrated photoactivation with CRISPR/Cas12a for DNA and RNA detection to provide high spatiotemporal control of nucleic acid sensing.
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage.
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage.
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage.
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage.
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage.
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage.
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage.
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
The photoactivatable CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets.
Our strategy suggests that the CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets
The photoactivatable CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets.
Our strategy suggests that the CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets
The photoactivatable CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets.
Our strategy suggests that the CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets
The photoactivatable CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets.
Our strategy suggests that the CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets
The photoactivatable CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets.
Our strategy suggests that the CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets
The photoactivatable CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets.
Our strategy suggests that the CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets
The photoactivatable CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets.
Our strategy suggests that the CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets
The photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin by rational design of the target recognition sequence.
By rationally designing the target recognition sequence, this photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin, respectively.
The photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin by rational design of the target recognition sequence.
By rationally designing the target recognition sequence, this photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin, respectively.
The photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin by rational design of the target recognition sequence.
By rationally designing the target recognition sequence, this photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin, respectively.
The photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin by rational design of the target recognition sequence.
By rationally designing the target recognition sequence, this photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin, respectively.
The photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin by rational design of the target recognition sequence.
By rationally designing the target recognition sequence, this photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin, respectively.
The photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin by rational design of the target recognition sequence.
By rationally designing the target recognition sequence, this photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin, respectively.
The photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin by rational design of the target recognition sequence.
By rationally designing the target recognition sequence, this photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin, respectively.
Approval Evidence
1 source1 linked approval claimfirst-pass slug lateral-flow-assay-strip-test-combined-with-crispr-cas12a
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals
Source:
assay capabilitysupports
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
Source:
Comparisons
Source-backed strengths
The reported strength is direct visualization of nucleic acid cleavage signals by combining lateral flow strips with CRISPR/Cas12a. The broader platform was described as enabling DNA and RNA detection, high spatiotemporal control through photoactivation, and rapid target nucleic acid detection in an in vivo survivin fluorescent sensing context.
Source:
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
Compared with Cas12aVIP
lateral flow assay strip test combined with CRISPR/Cas12a and Cas12aVIP address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Compared with light-controlled crRNA
lateral flow assay strip test combined with CRISPR/Cas12a and light-controlled crRNA address a similar problem space because they share editing.
Shared frame: shared target processes: editing; shared mechanisms: photocleavage
Compared with next-generation sequencing
lateral flow assay strip test combined with CRISPR/Cas12a and next-generation sequencing address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Relative tradeoffs: looks easier to implement in practice.
Ranked Citations
- 1.