Source 1primary paper2020
Toolkit/αSH2-OptoMB
αSH2-OptoMB
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
αSH2-OptoMB is a light-sensitive monobody in the OptoBinder class engineered to bind an SH2-domain target with an approximately 300-fold light-dependent affinity shift. It has been demonstrated as an affinity reagent for light-controlled purification of SH2-tagged proteins.
Usefulness & Problems
Why this is useful
αSH2-OptoMB is useful as a reversible, light-regulated affinity binder for SH2-tagged proteins, enabling dynamic control of target capture and release. The cited study demonstrates its use for direct purification from crude Escherichia coli extract in a single step.
Source:
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
Source:
a light-controlled monobody (OptoMB) that works in vitro and in vivo , whose affinity for its SH2-domain target exhibits a 300-fold shift in binding affinity upon illumination
Problem solved
This tool addresses the need for externally controllable protein binders that can modulate target affinity with light rather than only static binding. In the reported application, it solves single-step purification of SH2-tagged proteins from crude bacterial lysate.
Source:
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-dependent affinity switchinglight-dependent affinity switchingreversible target binding modulationreversible target binding modulationTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenoperating role: actuatorswitch architecture: multi component
The available evidence indicates use as an affinity reagent for SH2-tagged proteins and validation in crude E. coli extract. The supplied material does not provide construct architecture, chromophore requirements, expression conditions, or illumination wavelength details.
The supplied evidence supports purification of SH2-tagged proteins, but provides little detail here on broader target scope, kinetics, illumination parameters, or performance in other cellular contexts. The claim that OptoBinders could be designed for untagged proteins is presented as potential rather than direct validation for αSH2-OptoMB itself.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
purification steps 1 steppurity 99.8 %yield 40 %
OptoMB belongs to a class of light-sensitive protein binders called OptoBinders.
This OptoMB belongs to a new class of light-sensitive protein binders we call OptoBinders (OptoBNDRs)
OptoMB belongs to a class of light-sensitive protein binders called OptoBinders.
This OptoMB belongs to a new class of light-sensitive protein binders we call OptoBinders (OptoBNDRs)
OptoMB belongs to a class of light-sensitive protein binders called OptoBinders.
This OptoMB belongs to a new class of light-sensitive protein binders we call OptoBinders (OptoBNDRs)
OptoMB belongs to a class of light-sensitive protein binders called OptoBinders.
This OptoMB belongs to a new class of light-sensitive protein binders we call OptoBinders (OptoBNDRs)
OptoMB belongs to a class of light-sensitive protein binders called OptoBinders.
This OptoMB belongs to a new class of light-sensitive protein binders we call OptoBinders (OptoBNDRs)
OptoMB belongs to a class of light-sensitive protein binders called OptoBinders.
This OptoMB belongs to a new class of light-sensitive protein binders we call OptoBinders (OptoBNDRs)
OptoMB belongs to a class of light-sensitive protein binders called OptoBinders.
This OptoMB belongs to a new class of light-sensitive protein binders we call OptoBinders (OptoBNDRs)
OptoMB belongs to a class of light-sensitive protein binders called OptoBinders.
This OptoMB belongs to a new class of light-sensitive protein binders we call OptoBinders (OptoBNDRs)
OptoMB belongs to a class of light-sensitive protein binders called OptoBinders.
This OptoMB belongs to a new class of light-sensitive protein binders we call OptoBinders (OptoBNDRs)
OptoMB belongs to a class of light-sensitive protein binders called OptoBinders.
This OptoMB belongs to a new class of light-sensitive protein binders we call OptoBinders (OptoBNDRs)
OptoBinders have the potential to be designed to bind proteins of interest as light-switchable binders of untagged proteins with high affinity and selectivity.
OptoBinders (OptoBNDRs) which, by virtue of their ability to be designed to bind any protein of interest, have the potential to find new powerful applications as light-switchable binders of untagged proteins with high affinity and selectivity
OptoBinders have the potential to be designed to bind proteins of interest as light-switchable binders of untagged proteins with high affinity and selectivity.
OptoBinders (OptoBNDRs) which, by virtue of their ability to be designed to bind any protein of interest, have the potential to find new powerful applications as light-switchable binders of untagged proteins with high affinity and selectivity
OptoBinders have the potential to be designed to bind proteins of interest as light-switchable binders of untagged proteins with high affinity and selectivity.
OptoBinders (OptoBNDRs) which, by virtue of their ability to be designed to bind any protein of interest, have the potential to find new powerful applications as light-switchable binders of untagged proteins with high affinity and selectivity
OptoBinders have the potential to be designed to bind proteins of interest as light-switchable binders of untagged proteins with high affinity and selectivity.
OptoBinders (OptoBNDRs) which, by virtue of their ability to be designed to bind any protein of interest, have the potential to find new powerful applications as light-switchable binders of untagged proteins with high affinity and selectivity
OptoBinders have the potential to be designed to bind proteins of interest as light-switchable binders of untagged proteins with high affinity and selectivity.
OptoBinders (OptoBNDRs) which, by virtue of their ability to be designed to bind any protein of interest, have the potential to find new powerful applications as light-switchable binders of untagged proteins with high affinity and selectivity
OptoBinders have the potential to be designed to bind proteins of interest as light-switchable binders of untagged proteins with high affinity and selectivity.
OptoBinders (OptoBNDRs) which, by virtue of their ability to be designed to bind any protein of interest, have the potential to find new powerful applications as light-switchable binders of untagged proteins with high affinity and selectivity
OptoBinders have the potential to be designed to bind proteins of interest as light-switchable binders of untagged proteins with high affinity and selectivity.
OptoBinders (OptoBNDRs) which, by virtue of their ability to be designed to bind any protein of interest, have the potential to find new powerful applications as light-switchable binders of untagged proteins with high affinity and selectivity
OptoBinders have the potential to be designed to bind proteins of interest as light-switchable binders of untagged proteins with high affinity and selectivity.
OptoBinders (OptoBNDRs) which, by virtue of their ability to be designed to bind any protein of interest, have the potential to find new powerful applications as light-switchable binders of untagged proteins with high affinity and selectivity
OptoBinders have the potential to be designed to bind proteins of interest as light-switchable binders of untagged proteins with high affinity and selectivity.
OptoBinders (OptoBNDRs) which, by virtue of their ability to be designed to bind any protein of interest, have the potential to find new powerful applications as light-switchable binders of untagged proteins with high affinity and selectivity
OptoBinders have the potential to be designed to bind proteins of interest as light-switchable binders of untagged proteins with high affinity and selectivity.
OptoBinders (OptoBNDRs) which, by virtue of their ability to be designed to bind any protein of interest, have the potential to find new powerful applications as light-switchable binders of untagged proteins with high affinity and selectivity
OptoMB is a light-controlled monobody whose affinity for its SH2-domain target shifts by 300-fold upon illumination.
a light-controlled monobody (OptoMB) that works in vitro and in vivo , whose affinity for its SH2-domain target exhibits a 300-fold shift in binding affinity upon illumination
binding affinity shift upon illumination 300 fold
OptoMB is a light-controlled monobody whose affinity for its SH2-domain target shifts by 300-fold upon illumination.
a light-controlled monobody (OptoMB) that works in vitro and in vivo , whose affinity for its SH2-domain target exhibits a 300-fold shift in binding affinity upon illumination
binding affinity shift upon illumination 300 fold
OptoMB is a light-controlled monobody whose affinity for its SH2-domain target shifts by 300-fold upon illumination.
a light-controlled monobody (OptoMB) that works in vitro and in vivo , whose affinity for its SH2-domain target exhibits a 300-fold shift in binding affinity upon illumination
binding affinity shift upon illumination 300 fold
OptoMB is a light-controlled monobody whose affinity for its SH2-domain target shifts by 300-fold upon illumination.
a light-controlled monobody (OptoMB) that works in vitro and in vivo , whose affinity for its SH2-domain target exhibits a 300-fold shift in binding affinity upon illumination
binding affinity shift upon illumination 300 fold
OptoMB is a light-controlled monobody whose affinity for its SH2-domain target shifts by 300-fold upon illumination.
a light-controlled monobody (OptoMB) that works in vitro and in vivo , whose affinity for its SH2-domain target exhibits a 300-fold shift in binding affinity upon illumination
binding affinity shift upon illumination 300 fold
OptoMB is a light-controlled monobody whose affinity for its SH2-domain target shifts by 300-fold upon illumination.
a light-controlled monobody (OptoMB) that works in vitro and in vivo , whose affinity for its SH2-domain target exhibits a 300-fold shift in binding affinity upon illumination
binding affinity shift upon illumination 300 fold
OptoMB is a light-controlled monobody whose affinity for its SH2-domain target shifts by 300-fold upon illumination.
a light-controlled monobody (OptoMB) that works in vitro and in vivo , whose affinity for its SH2-domain target exhibits a 300-fold shift in binding affinity upon illumination
binding affinity shift upon illumination 300 fold
OptoMB is a light-controlled monobody whose affinity for its SH2-domain target shifts by 300-fold upon illumination.
a light-controlled monobody (OptoMB) that works in vitro and in vivo , whose affinity for its SH2-domain target exhibits a 300-fold shift in binding affinity upon illumination
binding affinity shift upon illumination 300 fold
OptoMB is a light-controlled monobody whose affinity for its SH2-domain target shifts by 300-fold upon illumination.
a light-controlled monobody (OptoMB) that works in vitro and in vivo , whose affinity for its SH2-domain target exhibits a 300-fold shift in binding affinity upon illumination
binding affinity shift upon illumination 300 fold
OptoMB is a light-controlled monobody whose affinity for its SH2-domain target shifts by 300-fold upon illumination.
a light-controlled monobody (OptoMB) that works in vitro and in vivo , whose affinity for its SH2-domain target exhibits a 300-fold shift in binding affinity upon illumination
binding affinity shift upon illumination 300 fold
Approval Evidence
1 source1 linked approval claimfirst-pass slug sh2-optomb
We demonstrate that our αSH2-OptoMB can be used to purify SH2-tagged proteins
Source:
applicationsupports
αSH2-OptoMB can purify SH2-tagged proteins directly from crude E. coli extract in a single purification step with 99.8% purity and over 40% yield.
our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step
Source:
Comparisons
Source-backed strengths
The reported performance includes an approximately 300-fold light-dependent change in affinity and successful purification of SH2-tagged proteins. In the cited purification experiment, the tool achieved 99.8% purity with over 40% yield in a single step from crude E. coli extract.
Compared with ArrayG
αSH2-OptoMB and ArrayG address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Compared with Opto-RhoGEFs
αSH2-OptoMB and Opto-RhoGEFs address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
αSH2-OptoMB and PmeR-OPmeR paraben-responsive mammalian transcription-control devices address a similar problem space.
Shared frame: same top-level item type
Ranked Citations
- 1.