Source 1review2010Cold Spring Harbor Perspectives in Biology
Toolkit/single-molecule imaging
single-molecule imaging
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Single-molecule imaging enables biophysical measurements devoid of ensemble averaging, gives enhanced spatial resolution beyond the diffraction limit, and permits superresolution reconstructions.
Usefulness & Problems
Why this is useful
Single-molecule imaging measures behavior of individual molecules rather than ensemble averages. In this review it is used in live bacteria to study protein diffusion, dynamics, and localization.; biophysical measurements without ensemble averaging; live bacterial protein imaging; measuring diffusion and dynamics of proteins in live cells
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Single-molecule imaging measures behavior of individual molecules rather than ensemble averages. In this review it is used in live bacteria to study protein diffusion, dynamics, and localization.
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biophysical measurements without ensemble averaging
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live bacterial protein imaging
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measuring diffusion and dynamics of proteins in live cells
Problem solved
It addresses the loss of information caused by ensemble averaging and allows direct biophysical measurements in live cells.; avoids ensemble averaging in biophysical measurements; supports observation of protein behavior in live bacterial cells
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It addresses the loss of information caused by ensemble averaging and allows direct biophysical measurements in live cells.
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avoids ensemble averaging in biophysical measurements
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supports observation of protein behavior in live bacterial cells
Problem links
avoids ensemble averaging in biophysical measurements
LiteratureIt addresses the loss of information caused by ensemble averaging and allows direct biophysical measurements in live cells.
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It addresses the loss of information caused by ensemble averaging and allows direct biophysical measurements in live cells.
supports observation of protein behavior in live bacterial cells
LiteratureIt addresses the loss of information caused by ensemble averaging and allows direct biophysical measurements in live cells.
Source:
It addresses the loss of information caused by ensemble averaging and allows direct biophysical measurements in live cells.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
localizationrecombinationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The abstract supports use in live Caulobacter crescentus cells and implies imaging-compatible protein labeling and microscopy, but does not specify instrumentation or labels.; applied here in live Caulobacter crescentus cells
The abstract does not describe specific throughput, labeling, or instrumentation limitations, so those boundaries are not extracted.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Single-molecule and superresolution imaging are applied in live Caulobacter crescentus cells to investigate PleC diffusion and dynamics, PopZ localization behavior, and MreB treadmilling behavior and protein superstructure with sub-40-nm spatial resolution.
spatial resolution 40 nm
Single-molecule imaging enables biophysical measurements without ensemble averaging, provides enhanced spatial resolution beyond the diffraction limit, and permits superresolution reconstructions.
Superresolution imaging provides enhanced spatial resolution beyond the diffraction limit and permits superresolution reconstructions in live bacterial cells.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug single-molecule-imaging
Single-molecule imaging enables biophysical measurements devoid of ensemble averaging, gives enhanced spatial resolution beyond the diffraction limit, and permits superresolution reconstructions.
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application scopesupports
Single-molecule and superresolution imaging are applied in live Caulobacter crescentus cells to investigate PleC diffusion and dynamics, PopZ localization behavior, and MreB treadmilling behavior and protein superstructure with sub-40-nm spatial resolution.
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capability summarysupports
Single-molecule imaging enables biophysical measurements without ensemble averaging, provides enhanced spatial resolution beyond the diffraction limit, and permits superresolution reconstructions.
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Comparisons
Source-stated alternatives
The abstract pairs single-molecule imaging with superresolution imaging as related approaches used in the review.
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The abstract pairs single-molecule imaging with superresolution imaging as related approaches used in the review.
Source-backed strengths
enables biophysical measurements devoid of ensemble averaging; permits enhanced spatial resolution beyond the diffraction limit; permits superresolution reconstructions
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enables biophysical measurements devoid of ensemble averaging
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permits enhanced spatial resolution beyond the diffraction limit
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permits superresolution reconstructions
Compared with imaging
The abstract pairs single-molecule imaging with superresolution imaging as related approaches used in the review.
Shared frame: source-stated alternative in extracted literature
Strengths here: enables biophysical measurements devoid of ensemble averaging; permits enhanced spatial resolution beyond the diffraction limit; permits superresolution reconstructions.
Source:
The abstract pairs single-molecule imaging with superresolution imaging as related approaches used in the review.
Compared with imaging surveillance
The abstract pairs single-molecule imaging with superresolution imaging as related approaches used in the review.
Shared frame: source-stated alternative in extracted literature
Strengths here: enables biophysical measurements devoid of ensemble averaging; permits enhanced spatial resolution beyond the diffraction limit; permits superresolution reconstructions.
Source:
The abstract pairs single-molecule imaging with superresolution imaging as related approaches used in the review.
Compared with superresolution imaging
The abstract pairs single-molecule imaging with superresolution imaging as related approaches used in the review.
Shared frame: source-stated alternative in extracted literature
Strengths here: enables biophysical measurements devoid of ensemble averaging; permits enhanced spatial resolution beyond the diffraction limit; permits superresolution reconstructions.
Source:
The abstract pairs single-molecule imaging with superresolution imaging as related approaches used in the review.
Ranked Citations
- 1.