Source 1primary paper2020Nature Communications
Toolkit/small molecule regulated sgRNAs
small molecule regulated sgRNAs
Also known as: regulated sgRNAs
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Small molecule regulated sgRNAs are engineered single-guide RNAs that confer small-molecule control over Cas9-mediated genome editing in Escherichia coli. The available evidence supports their function as RNA-level regulators of CRISPR-Cas9 editing activity in a bacterial system.
Usefulness & Problems
Why this is useful
These regulated sgRNAs are useful for making Cas9 genome editing conditional on the presence of a small molecule in E. coli. This provides a way to modulate editing activity at the guide RNA level rather than only through Cas9 protein expression.
Source:
Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
Problem solved
This tool addresses the problem of controlling when Cas9-mediated genome editing occurs in E. coli. The evidence indicates that engineered sgRNAs can couple editing output to a small-molecule input.
Problem links
Need controllable genome or transcript editing
DerivedSmall molecule regulated sgRNAs are engineered single-guide RNAs that confer small-molecule control over Cas9-mediated genome editing in Escherichia coli. The available evidence supports their function as RNA-level regulators of CRISPR-Cas9 editing activity in a bacterial system.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level RNA part used inside a larger architecture that realizes a mechanism.
Mechanisms
rna-level control of cas9-mediated genome editingsmall-molecule-dependent regulation of sgrna activityTechniques
No technique tags yet.
Target processes
editingImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: regulator
The tool is described as an engineered sgRNA used with Cas9 in E. coli. The supplied evidence does not specify construct architecture, ligand identity, expression strategy, or delivery details.
The supplied evidence is limited to a single high-level claim and does not specify the small molecule, dynamic range, leakiness, sequence constraints, or portability beyond E. coli. No independent replication or broader validation context is provided in the supplied material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Small molecule regulated sgRNAs enable control of Cas9-mediated genome editing in E. coli.
Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
Small molecule regulated sgRNAs enable control of Cas9-mediated genome editing in E. coli.
Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
Small molecule regulated sgRNAs enable control of Cas9-mediated genome editing in E. coli.
Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
Small molecule regulated sgRNAs enable control of Cas9-mediated genome editing in E. coli.
Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
Small molecule regulated sgRNAs enable control of Cas9-mediated genome editing in E. coli.
Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
Small molecule regulated sgRNAs enable control of Cas9-mediated genome editing in E. coli.
Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
Small molecule regulated sgRNAs enable control of Cas9-mediated genome editing in E. coli.
Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
Approval Evidence
1 source1 linked approval claimfirst-pass slug small-molecule-regulated-sgrnas
Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
Source:
functional capabilitysupports
Small molecule regulated sgRNAs enable control of Cas9-mediated genome editing in E. coli.
Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
Source:
Comparisons
Source-backed strengths
The reported strength is the ability to control Cas9-mediated genome editing in E. coli using engineered sgRNAs and a small-molecule signal. The current evidence does not provide additional quantitative performance details in the supplied material.
Compared with antisense oligonucleotides
small molecule regulated sgRNAs and antisense oligonucleotides address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Strengths here: looks easier to implement in practice.
Compared with photo-sensitive circular gRNAs
small molecule regulated sgRNAs and photo-sensitive circular gRNAs address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Strengths here: looks easier to implement in practice.
Compared with synthetic riboswitches
small molecule regulated sgRNAs and synthetic riboswitches address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.