Source 1primary paper2018International Journal of Molecular Sciences
Toolkit/SPI1085g3
SPI1085g3
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
SPI1085g3 is a red/green cyanobacteriochrome GAF domain from Spirulina subsalsa. It photoconverts from a red-absorbing dark Pr state to an orange-absorbing Po state, and the Pr state exhibits intense red fluorescence that is lost upon photoconversion and recovers through moderate dark reversion.
Usefulness & Problems
Why this is useful
SPI1085g3 is useful as a light-responsive protein domain that couples spectral state switching to red fluorescence output. This combination supports applications requiring optical readout of protein state and reversible control by light and dark recovery, although the supplied evidence does not directly validate a specific downstream use case.
Source:
This novel red/dark-switchable fluorescent biliprotein expands the present repertoire and diversity of photoswitchable fluorescent protein candidates.
Problem solved
SPI1085g3 addresses the need for a photoswitchable fluorescent cyanobacteriochrome domain with reversible interconversion between a fluorescent Pr state and a nonfluorescent or less fluorescent Po state. The evidence supports state switching and dark recovery, but does not provide direct data on recombination control despite that listed target process.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Techniques
Computational DesignTarget processes
recombinationInput: Light
Implementation Constraints
SPI1085g3 is described as a cyanobacteriochrome GAF domain from Spirulina subsalsa, so implementation would involve expression of this protein domain rather than a full-length photoreceptor. The supplied evidence does not specify construct architecture, chromophore/cofactor usage, host system, illumination parameters, or delivery strategy.
The evidence is limited to a single 2018 study and a small set of photophysical claims. No quantitative wavelengths, chromophore requirements, kinetics, dynamic range, cellular validation, or direct demonstration in recombination assays are provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
SPI1085g3 exhibits moderate dark reversion from the Po state to the Pr state.
exhibited moderate dark reversion (t1/2 = 3.3 min) from the Po state to the Pr state
dark reversion half-life 3.3 min
SPI1085g3 exhibits moderate dark reversion from the Po state to the Pr state.
exhibited moderate dark reversion (t1/2 = 3.3 min) from the Po state to the Pr state
dark reversion half-life 3.3 min
SPI1085g3 exhibits moderate dark reversion from the Po state to the Pr state.
exhibited moderate dark reversion (t1/2 = 3.3 min) from the Po state to the Pr state
dark reversion half-life 3.3 min
SPI1085g3 exhibits moderate dark reversion from the Po state to the Pr state.
exhibited moderate dark reversion (t1/2 = 3.3 min) from the Po state to the Pr state
dark reversion half-life 3.3 min
SPI1085g3 exhibits moderate dark reversion from the Po state to the Pr state.
exhibited moderate dark reversion (t1/2 = 3.3 min) from the Po state to the Pr state
dark reversion half-life 3.3 min
SPI1085g3 exhibits moderate dark reversion from the Po state to the Pr state.
exhibited moderate dark reversion (t1/2 = 3.3 min) from the Po state to the Pr state
dark reversion half-life 3.3 min
SPI1085g3 exhibits moderate dark reversion from the Po state to the Pr state.
exhibited moderate dark reversion (t1/2 = 3.3 min) from the Po state to the Pr state
dark reversion half-life 3.3 min
The Pr state of SPI1085g3 exhibits intense red fluorescence.
The SPI1085g3 Pr state exhibited intense red fluorescence (λmax = 662 nm), with a quantum yield of 0.14.
fluorescence emission maximum 662 nmfluorescence quantum yield 0.14
The Pr state of SPI1085g3 exhibits intense red fluorescence.
The SPI1085g3 Pr state exhibited intense red fluorescence (λmax = 662 nm), with a quantum yield of 0.14.
fluorescence emission maximum 662 nmfluorescence quantum yield 0.14
The Pr state of SPI1085g3 exhibits intense red fluorescence.
The SPI1085g3 Pr state exhibited intense red fluorescence (λmax = 662 nm), with a quantum yield of 0.14.
fluorescence emission maximum 662 nmfluorescence quantum yield 0.14
The Pr state of SPI1085g3 exhibits intense red fluorescence.
The SPI1085g3 Pr state exhibited intense red fluorescence (λmax = 662 nm), with a quantum yield of 0.14.
fluorescence emission maximum 662 nmfluorescence quantum yield 0.14
The Pr state of SPI1085g3 exhibits intense red fluorescence.
The SPI1085g3 Pr state exhibited intense red fluorescence (λmax = 662 nm), with a quantum yield of 0.14.
fluorescence emission maximum 662 nmfluorescence quantum yield 0.14
The Pr state of SPI1085g3 exhibits intense red fluorescence.
The SPI1085g3 Pr state exhibited intense red fluorescence (λmax = 662 nm), with a quantum yield of 0.14.
fluorescence emission maximum 662 nmfluorescence quantum yield 0.14
The Pr state of SPI1085g3 exhibits intense red fluorescence.
The SPI1085g3 Pr state exhibited intense red fluorescence (λmax = 662 nm), with a quantum yield of 0.14.
fluorescence emission maximum 662 nmfluorescence quantum yield 0.14
SPI1085g3 photoconverts from a red-absorbing dark Pr state to an orange-absorbing Po photoproduct state.
SPI1085g3, which exhibited photoconversion from the red-absorbing dark state (Pr, λmax = 642 nm) to the orange-absorbing photoproduct state (Po, λmax = 590 nm)
Po absorption maximum 590 nmPr absorption maximum 642 nm
SPI1085g3 photoconverts from a red-absorbing dark Pr state to an orange-absorbing Po photoproduct state.
SPI1085g3, which exhibited photoconversion from the red-absorbing dark state (Pr, λmax = 642 nm) to the orange-absorbing photoproduct state (Po, λmax = 590 nm)
Po absorption maximum 590 nmPr absorption maximum 642 nm
SPI1085g3 photoconverts from a red-absorbing dark Pr state to an orange-absorbing Po photoproduct state.
SPI1085g3, which exhibited photoconversion from the red-absorbing dark state (Pr, λmax = 642 nm) to the orange-absorbing photoproduct state (Po, λmax = 590 nm)
Po absorption maximum 590 nmPr absorption maximum 642 nm
SPI1085g3 photoconverts from a red-absorbing dark Pr state to an orange-absorbing Po photoproduct state.
SPI1085g3, which exhibited photoconversion from the red-absorbing dark state (Pr, λmax = 642 nm) to the orange-absorbing photoproduct state (Po, λmax = 590 nm)
Po absorption maximum 590 nmPr absorption maximum 642 nm
SPI1085g3 photoconverts from a red-absorbing dark Pr state to an orange-absorbing Po photoproduct state.
SPI1085g3, which exhibited photoconversion from the red-absorbing dark state (Pr, λmax = 642 nm) to the orange-absorbing photoproduct state (Po, λmax = 590 nm)
Po absorption maximum 590 nmPr absorption maximum 642 nm
SPI1085g3 photoconverts from a red-absorbing dark Pr state to an orange-absorbing Po photoproduct state.
SPI1085g3, which exhibited photoconversion from the red-absorbing dark state (Pr, λmax = 642 nm) to the orange-absorbing photoproduct state (Po, λmax = 590 nm)
Po absorption maximum 590 nmPr absorption maximum 642 nm
SPI1085g3 photoconverts from a red-absorbing dark Pr state to an orange-absorbing Po photoproduct state.
SPI1085g3, which exhibited photoconversion from the red-absorbing dark state (Pr, λmax = 642 nm) to the orange-absorbing photoproduct state (Po, λmax = 590 nm)
Po absorption maximum 590 nmPr absorption maximum 642 nm
SPI1085g3 fluorescence is switched off by red light irradiation and increases in the dark.
The fluorescence was switched off by red light irradiation and increased in the dark.
SPI1085g3 fluorescence is switched off by red light irradiation and increases in the dark.
The fluorescence was switched off by red light irradiation and increased in the dark.
SPI1085g3 fluorescence is switched off by red light irradiation and increases in the dark.
The fluorescence was switched off by red light irradiation and increased in the dark.
SPI1085g3 fluorescence is switched off by red light irradiation and increases in the dark.
The fluorescence was switched off by red light irradiation and increased in the dark.
SPI1085g3 fluorescence is switched off by red light irradiation and increases in the dark.
The fluorescence was switched off by red light irradiation and increased in the dark.
SPI1085g3 fluorescence is switched off by red light irradiation and increases in the dark.
The fluorescence was switched off by red light irradiation and increased in the dark.
SPI1085g3 fluorescence is switched off by red light irradiation and increases in the dark.
The fluorescence was switched off by red light irradiation and increased in the dark.
SPI1085g3 is a novel red/dark-switchable fluorescent biliprotein and a photoswitchable fluorescent protein candidate.
This novel red/dark-switchable fluorescent biliprotein expands the present repertoire and diversity of photoswitchable fluorescent protein candidates.
SPI1085g3 is a novel red/dark-switchable fluorescent biliprotein and a photoswitchable fluorescent protein candidate.
This novel red/dark-switchable fluorescent biliprotein expands the present repertoire and diversity of photoswitchable fluorescent protein candidates.
SPI1085g3 is a novel red/dark-switchable fluorescent biliprotein and a photoswitchable fluorescent protein candidate.
This novel red/dark-switchable fluorescent biliprotein expands the present repertoire and diversity of photoswitchable fluorescent protein candidates.
SPI1085g3 is a novel red/dark-switchable fluorescent biliprotein and a photoswitchable fluorescent protein candidate.
This novel red/dark-switchable fluorescent biliprotein expands the present repertoire and diversity of photoswitchable fluorescent protein candidates.
SPI1085g3 is a novel red/dark-switchable fluorescent biliprotein and a photoswitchable fluorescent protein candidate.
This novel red/dark-switchable fluorescent biliprotein expands the present repertoire and diversity of photoswitchable fluorescent protein candidates.
SPI1085g3 is a novel red/dark-switchable fluorescent biliprotein and a photoswitchable fluorescent protein candidate.
This novel red/dark-switchable fluorescent biliprotein expands the present repertoire and diversity of photoswitchable fluorescent protein candidates.
SPI1085g3 is a novel red/dark-switchable fluorescent biliprotein and a photoswitchable fluorescent protein candidate.
This novel red/dark-switchable fluorescent biliprotein expands the present repertoire and diversity of photoswitchable fluorescent protein candidates.
The SPI1085g3 Cys448Ser variant has slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion than wild type.
Replacement of Cys448 of SPI1085g3 with Ser resulted in a slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion (t1/2 = 15.2 s) than that of the wild type.
dark reversion half-life 15.2 sdark reversion rate change versus wild type nearly 13-fold faster
The SPI1085g3 Cys448Ser variant has slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion than wild type.
Replacement of Cys448 of SPI1085g3 with Ser resulted in a slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion (t1/2 = 15.2 s) than that of the wild type.
dark reversion half-life 15.2 sdark reversion rate change versus wild type nearly 13-fold faster
The SPI1085g3 Cys448Ser variant has slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion than wild type.
Replacement of Cys448 of SPI1085g3 with Ser resulted in a slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion (t1/2 = 15.2 s) than that of the wild type.
dark reversion half-life 15.2 sdark reversion rate change versus wild type nearly 13-fold faster
The SPI1085g3 Cys448Ser variant has slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion than wild type.
Replacement of Cys448 of SPI1085g3 with Ser resulted in a slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion (t1/2 = 15.2 s) than that of the wild type.
dark reversion half-life 15.2 sdark reversion rate change versus wild type nearly 13-fold faster
The SPI1085g3 Cys448Ser variant has slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion than wild type.
Replacement of Cys448 of SPI1085g3 with Ser resulted in a slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion (t1/2 = 15.2 s) than that of the wild type.
dark reversion half-life 15.2 sdark reversion rate change versus wild type nearly 13-fold faster
The SPI1085g3 Cys448Ser variant has slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion than wild type.
Replacement of Cys448 of SPI1085g3 with Ser resulted in a slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion (t1/2 = 15.2 s) than that of the wild type.
dark reversion half-life 15.2 sdark reversion rate change versus wild type nearly 13-fold faster
The SPI1085g3 Cys448Ser variant has slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion than wild type.
Replacement of Cys448 of SPI1085g3 with Ser resulted in a slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion (t1/2 = 15.2 s) than that of the wild type.
dark reversion half-life 15.2 sdark reversion rate change versus wild type nearly 13-fold faster
Approval Evidence
1 source5 linked approval claimsfirst-pass slug spi1085g3
Here, we report a red/green cyanobacteriochrome GAF domain from Spirulina subsalsa, designated SPI1085g3
Source:
dark reversion propertysupports
SPI1085g3 exhibits moderate dark reversion from the Po state to the Pr state.
exhibited moderate dark reversion (t1/2 = 3.3 min) from the Po state to the Pr state
Source:
fluorescence propertysupports
The Pr state of SPI1085g3 exhibits intense red fluorescence.
The SPI1085g3 Pr state exhibited intense red fluorescence (λmax = 662 nm), with a quantum yield of 0.14.
Source:
photoconversion propertysupports
SPI1085g3 photoconverts from a red-absorbing dark Pr state to an orange-absorbing Po photoproduct state.
SPI1085g3, which exhibited photoconversion from the red-absorbing dark state (Pr, λmax = 642 nm) to the orange-absorbing photoproduct state (Po, λmax = 590 nm)
Source:
photoswitching behaviorsupports
SPI1085g3 fluorescence is switched off by red light irradiation and increases in the dark.
The fluorescence was switched off by red light irradiation and increased in the dark.
Source:
tool characterizationsupports
SPI1085g3 is a novel red/dark-switchable fluorescent biliprotein and a photoswitchable fluorescent protein candidate.
This novel red/dark-switchable fluorescent biliprotein expands the present repertoire and diversity of photoswitchable fluorescent protein candidates.
Source:
Comparisons
Source-backed strengths
The reported strengths are intense red fluorescence in the Pr state, photoconversion from Pr to Po, and moderate dark reversion from Po back to Pr. These properties indicate a reversible optical switch with intrinsic fluorescence reporting in a compact GAF-domain format.
Ranked Citations
- 1.