Here, we measured phototransformation quantum yields for Dendra2 fused to actin in fixed mammalian cells in typical (F)-PALM experiments.
Home/Mechanisms/photoconversion
Mechanism Concept
photoconversion
A mechanism-level grouping derived from the current toolkit evidence. Current coverage includes 1 architecture and 2 components. Across those tools, the main enabled capabilities are recombination. Representative components include SPI1085g3 and SPI1085g3 Cys448Ser variant.
3 total items1 architectures2 components
Main enabled capabilities: recombination.
Architectures
1 of 1Components
2 of 2SPI1085g3 Cys448Ser variant is a cyanobacteriochrome GAF-domain mutant derived from the Spirulina protein SPI1085g3, generated by replacing Cys448 with Ser. In the reported study, this variant retained the photoswitchable fluorescent behavior of SPI1085g3 while showing slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion.
SPI1085g3 is a red/green cyanobacteriochrome GAF domain from Spirulina subsalsa. It photoconverts from a red-absorbing dark Pr state to an orange-absorbing Po state, and the Pr state exhibits intense red fluorescence that is lost upon photoconversion and recovers through moderate dark reversion.