Source 1primary paper2019Nature Communications
Toolkit/super-linear excitation-emission microscopy
super-linear excitation-emission microscopy
Also known as: SEE microscopy
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The paper explicitly introduces super-linear excitation-emission (SEE) microscopy.
Usefulness & Problems
Why this is useful
SEE microscopy uses super-linear emitter responses to achieve 3D sub-diffraction imaging in a conventional confocal configuration.; 3D sub-diffraction imaging in a conventional confocal configuration
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SEE microscopy uses super-linear emitter responses to achieve 3D sub-diffraction imaging in a conventional confocal configuration.
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3D sub-diffraction imaging in a conventional confocal configuration
Problem solved
It addresses how to obtain sub-diffraction imaging without departing from a conventional confocal setup.; Obtaining sub-diffraction imaging by exploiting super-linear emitters in a conventional confocal setup
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It addresses how to obtain sub-diffraction imaging without departing from a conventional confocal setup.
Source:
Obtaining sub-diffraction imaging by exploiting super-linear emitters in a conventional confocal setup
Problem links
Obtaining sub-diffraction imaging by exploiting super-linear emitters in a conventional confocal setup
LiteratureIt addresses how to obtain sub-diffraction imaging without departing from a conventional confocal setup.
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It addresses how to obtain sub-diffraction imaging without departing from a conventional confocal setup.
Published Workflows
Objective: Achieve 3D sub-diffraction imaging in a conventional confocal configuration by exploiting super-linear emitters.
Why it works: The method is reported to exploit super-linear emitters so that a conventional confocal configuration can produce sub-diffraction imaging.
super-linear excitation-emission behaviorupconversion emissionconfocal imaginguse of super-linear emitters
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
It requires a confocal microscope configuration and emitters with super-linear excitation-emission behavior.; Requires super-linear emitters
Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The paper implements uSEE microscopy using NaYF4:20%Yb,8%Tm upconversion nanoparticles excited at 976 nm with 455 nm emission for low-power 3D sub-diffraction imaging in a conventional confocal setup.
emission wavelength 455 nmexcitation wavelength 976 nm
The paper introduces super-linear excitation-emission microscopy as a method for 3D sub-diffraction imaging in a conventional confocal configuration.
The paper uses the term uSEE microscopy for the implementation of SEE microscopy with upconversion nanoparticles.
Approval Evidence
1 source1 linked approval claimfirst-pass slug super-linear-excitation-emission-microscopy
The paper explicitly introduces super-linear excitation-emission (SEE) microscopy.
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method introductionsupports
The paper introduces super-linear excitation-emission microscopy as a method for 3D sub-diffraction imaging in a conventional confocal configuration.
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Comparisons
Source-stated alternatives
The web research summary places SEE alongside other UCNP-based super-resolution modalities such as multiphoton near-infrared emission saturation nanoscopy and nonlinear structured illumination approaches.
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The web research summary places SEE alongside other UCNP-based super-resolution modalities such as multiphoton near-infrared emission saturation nanoscopy and nonlinear structured illumination approaches.
Source-backed strengths
Works in a conventional confocal configuration; Supports 3D sub-diffraction imaging
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Works in a conventional confocal configuration
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Supports 3D sub-diffraction imaging
Compared with structured illumination
The web research summary places SEE alongside other UCNP-based super-resolution modalities such as multiphoton near-infrared emission saturation nanoscopy and nonlinear structured illumination approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: Works in a conventional confocal configuration; Supports 3D sub-diffraction imaging.
Source:
The web research summary places SEE alongside other UCNP-based super-resolution modalities such as multiphoton near-infrared emission saturation nanoscopy and nonlinear structured illumination approaches.
Compared with super-resolution microscopy
The web research summary places SEE alongside other UCNP-based super-resolution modalities such as multiphoton near-infrared emission saturation nanoscopy and nonlinear structured illumination approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: Works in a conventional confocal configuration; Supports 3D sub-diffraction imaging.
Source:
The web research summary places SEE alongside other UCNP-based super-resolution modalities such as multiphoton near-infrared emission saturation nanoscopy and nonlinear structured illumination approaches.
Ranked Citations
- 1.