Source 1review2022Annual Review of Analytical Chemistry
Toolkit/structured illumination
structured illumination
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
These label-free techniques span a variety of different approaches, including structured illumination, transient absorption, infrared absorption, and coherent Raman spectroscopies.
Usefulness & Problems
Why this is useful
Structured illumination is presented as one of the label-free approaches used to achieve super-resolution imaging. The review places it within a broader set of methods that seek to resolve nanoscale heterogeneity without labels.; label-free super-resolution imaging
Source:
Structured illumination is presented as one of the label-free approaches used to achieve super-resolution imaging. The review places it within a broader set of methods that seek to resolve nanoscale heterogeneity without labels.
Source:
label-free super-resolution imaging
Problem solved
It addresses the need to image nanoscale features that conventional microscopy cannot resolve while avoiding fluorescent labeling.; obtaining super-resolution information without fluorescent labels
Source:
It addresses the need to image nanoscale features that conventional microscopy cannot resolve while avoiding fluorescent labeling.
Source:
obtaining super-resolution information without fluorescent labels
Problem links
obtaining super-resolution information without fluorescent labels
LiteratureIt addresses the need to image nanoscale features that conventional microscopy cannot resolve while avoiding fluorescent labeling.
Source:
It addresses the need to image nanoscale features that conventional microscopy cannot resolve while avoiding fluorescent labeling.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
structured illuminationTechniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
Many label-free super-resolution methods draw inspiration from fluorescence-based STED, PALM, and STORM.
The abstract does not state whether it reaches fluorescence-method-level resolution or what sample classes are best served.; the abstract does not specify performance limits or implementation details
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Super-resolution fluorescence methods can break the optical diffraction limit but require fluorescent labeling.
Many label-free super-resolution methods draw inspiration from fluorescence-based STED, PALM, and STORM.
Label-free super-resolution techniques include structured illumination, transient absorption, infrared absorption, and coherent Raman spectroscopies.
Approval Evidence
1 source1 linked approval claimfirst-pass slug structured-illumination
These label-free techniques span a variety of different approaches, including structured illumination, transient absorption, infrared absorption, and coherent Raman spectroscopies.
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field summarysupports
Label-free super-resolution techniques include structured illumination, transient absorption, infrared absorption, and coherent Raman spectroscopies.
Source:
Comparisons
Source-stated alternatives
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Source:
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Source-backed strengths
presented as a label-free route to super-resolution imaging
Source:
presented as a label-free route to super-resolution imaging
Compared with coherent Raman super-resolution imaging
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free route to super-resolution imaging.
Relative tradeoffs: the abstract does not specify performance limits or implementation details.
Source:
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with infrared absorption super-resolution imaging
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free route to super-resolution imaging.
Relative tradeoffs: the abstract does not specify performance limits or implementation details.
Source:
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with PALM
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free route to super-resolution imaging.
Relative tradeoffs: the abstract does not specify performance limits or implementation details.
Source:
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with photoactivated localization microscopy
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free route to super-resolution imaging.
Relative tradeoffs: the abstract does not specify performance limits or implementation details.
Source:
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with photo-activation localization microscopy
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free route to super-resolution imaging.
Relative tradeoffs: the abstract does not specify performance limits or implementation details.
Source:
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with photoactivation localization microscopy
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free route to super-resolution imaging.
Relative tradeoffs: the abstract does not specify performance limits or implementation details.
Source:
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with STED
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free route to super-resolution imaging.
Relative tradeoffs: the abstract does not specify performance limits or implementation details.
Source:
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with STED microscopy
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free route to super-resolution imaging.
Relative tradeoffs: the abstract does not specify performance limits or implementation details.
Source:
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with stimulated emission depletion microscopy
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free route to super-resolution imaging.
Relative tradeoffs: the abstract does not specify performance limits or implementation details.
Source:
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with stochastic optical reconstruction microscopy
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free route to super-resolution imaging.
Relative tradeoffs: the abstract does not specify performance limits or implementation details.
Source:
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with STORM
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free route to super-resolution imaging.
Relative tradeoffs: the abstract does not specify performance limits or implementation details.
Source:
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with transient absorption super-resolution imaging
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free route to super-resolution imaging.
Relative tradeoffs: the abstract does not specify performance limits or implementation details.
Source:
The abstract contrasts it with transient absorption, infrared absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Ranked Citations
- 1.