Source 1primary paper2019Scientific Reports
Toolkit/super-resolution cryogenic correlative light and electron microscopy
super-resolution cryogenic correlative light and electron microscopy
Also known as: SR-cryoCLEM
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
web_research_summary states the paper describes super-resolution cryogenic correlative light and electron microscopy (SR-cryoCLEM) in mammalian cells using reversibly switchable fluorescent proteins.
Usefulness & Problems
Why this is useful
This method combines cryogenic super-resolution fluorescence imaging with electron microscopy correlation in mammalian cells. The source summary identifies it as SR-cryoCLEM using fluorescent proteins.; correlating super-resolution fluorescence and electron microscopy in cryogenic mammalian-cell samples; localizing fluorescently labeled structures for cryo-electron microscopy workflows
Source:
This method combines cryogenic super-resolution fluorescence imaging with electron microscopy correlation in mammalian cells. The source summary identifies it as SR-cryoCLEM using fluorescent proteins.
Source:
correlating super-resolution fluorescence and electron microscopy in cryogenic mammalian-cell samples
Source:
localizing fluorescently labeled structures for cryo-electron microscopy workflows
Problem solved
It addresses the need to localize fluorescently labeled cellular structures with higher precision in cryogenic correlative microscopy.; improves fluorescent targeting/localization within cryogenic correlative light and electron microscopy
Source:
It addresses the need to localize fluorescently labeled cellular structures with higher precision in cryogenic correlative microscopy.
Source:
improves fluorescent targeting/localization within cryogenic correlative light and electron microscopy
Problem links
improves fluorescent targeting/localization within cryogenic correlative light and electron microscopy
LiteratureIt addresses the need to localize fluorescently labeled cellular structures with higher precision in cryogenic correlative microscopy.
Source:
It addresses the need to localize fluorescently labeled cellular structures with higher precision in cryogenic correlative microscopy.
Published Workflows
Objective: Perform super-resolution cryogenic correlative light and electron microscopy in mammalian cells using fluorescent proteins.
Why it works: The workflow is described as combining super-resolution fluorescence with electron microscopy correlation under cryogenic conditions, using fluorescent proteins to localize structures in mammalian cells.
reversibly switchable fluorescent protein imaging under cryogenic conditionssuper-resolution cryogenic correlative light and electron microscopy
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
localizationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
It requires cryogenic correlative light and electron microscopy instrumentation and fluorescent protein labeling compatible with cryogenic imaging.; requires cryogenic correlative light and electron microscopy setup; requires compatible fluorescent protein labels
Needs compatible illumination hardware and optical access. Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The study notably used rsEGFP2-MAP2 in U2OS cells.
web_research_summary: notably rsEGFP2-MAP2 in U2OS cells
This paper describes super-resolution cryogenic correlative light and electron microscopy in mammalian cells using reversibly switchable fluorescent proteins.
web_research_summary: describes super-resolution cryogenic correlative light and electron microscopy (SR-cryoCLEM) in mammalian cells using reversibly switchable fluorescent proteins
The reported localization precision was around 27 nm.
web_research_summary: with cited localization precision around 27 nm
localization precision 27 nm
Approval Evidence
1 source2 linked approval claimsfirst-pass slug super-resolution-cryogenic-correlative-light-and-electron-microscopy
web_research_summary states the paper describes super-resolution cryogenic correlative light and electron microscopy (SR-cryoCLEM) in mammalian cells using reversibly switchable fluorescent proteins.
Source:
method applicationsupports
This paper describes super-resolution cryogenic correlative light and electron microscopy in mammalian cells using reversibly switchable fluorescent proteins.
web_research_summary: describes super-resolution cryogenic correlative light and electron microscopy (SR-cryoCLEM) in mammalian cells using reversibly switchable fluorescent proteins
Source:
performancesupports
The reported localization precision was around 27 nm.
web_research_summary: with cited localization precision around 27 nm
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Comparisons
Source-stated alternatives
The source summary points to precursor cryogenic super-resolution methods using standard fluorescent proteins and adjacent cryo-CLEM workflow papers as related approaches.
Source:
The source summary points to precursor cryogenic super-resolution methods using standard fluorescent proteins and adjacent cryo-CLEM workflow papers as related approaches.
Source-backed strengths
combines super-resolution fluorescence with electron microscopy under cryogenic conditions; applied in mammalian cells using fluorescent proteins
Source:
combines super-resolution fluorescence with electron microscopy under cryogenic conditions
Source:
applied in mammalian cells using fluorescent proteins
Compared with correlative light and electron microscopy
The source summary points to precursor cryogenic super-resolution methods using standard fluorescent proteins and adjacent cryo-CLEM workflow papers as related approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: combines super-resolution fluorescence with electron microscopy under cryogenic conditions; applied in mammalian cells using fluorescent proteins.
Source:
The source summary points to precursor cryogenic super-resolution methods using standard fluorescent proteins and adjacent cryo-CLEM workflow papers as related approaches.
Compared with Correlative light-electron microscopy
The source summary points to precursor cryogenic super-resolution methods using standard fluorescent proteins and adjacent cryo-CLEM workflow papers as related approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: combines super-resolution fluorescence with electron microscopy under cryogenic conditions; applied in mammalian cells using fluorescent proteins.
Source:
The source summary points to precursor cryogenic super-resolution methods using standard fluorescent proteins and adjacent cryo-CLEM workflow papers as related approaches.
Ranked Citations
- 1.