Source 1review2010Biophysical Reviews
Toolkit/super-resolution fluorescence microscopy
super-resolution fluorescence microscopy
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
In recent years, however, a variety of super-resolution fluorescence microscopy techniques have been developed that circumvent the resolution limitation.
Usefulness & Problems
Why this is useful
This is a family of fluorescence microscopy techniques that overcomes the resolution limit of regular optical microscopy for nanoscale cellular imaging.; imaging subcellular structures below the diffraction limit; studying molecular processes at spatial scales between 1 and 100 nm; cellular biophysics imaging
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This is a family of fluorescence microscopy techniques that overcomes the resolution limit of regular optical microscopy for nanoscale cellular imaging.
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imaging subcellular structures below the diffraction limit
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studying molecular processes at spatial scales between 1 and 100 nm
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cellular biophysics imaging
Problem solved
It addresses the inability of regular optical microscopy to study molecular processes occurring at roughly 1-100 nm spatial scales.; circumventing the ~200 nm resolution limit of regular optical microscopy
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It addresses the inability of regular optical microscopy to study molecular processes occurring at roughly 1-100 nm spatial scales.
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circumventing the ~200 nm resolution limit of regular optical microscopy
Problem links
circumventing the ~200 nm resolution limit of regular optical microscopy
LiteratureIt addresses the inability of regular optical microscopy to study molecular processes occurring at roughly 1-100 nm spatial scales.
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It addresses the inability of regular optical microscopy to study molecular processes occurring at roughly 1-100 nm spatial scales.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
No mechanism tags yet.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The abstract supports that fluorescence labeling with fluorophores is required, within an optical microscopy setup.; requires fluorescence microscopy; requires specific labeling with fluorophores
The abstract does not specify which super-resolution methods are best for particular samples, speeds, or imaging depths.; specific technique families and tradeoffs are not detailed in the abstract
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Fluorescence microscopy enables specific labeling of biomolecules or supramolecular structures with fluorophores so that images report on processes involving the labeled molecules.
The use of fluorescence microscopy is advantageous because biomolecules or supramolecular structures of interest can be labeled specifically with fluorophores, so the images reveal information on processes involving only the labeled molecules.
Super-resolution fluorescence microscopy techniques have been developed to circumvent the resolution limitation of regular optical microscopy.
In recent years, however, a variety of super-resolution fluorescence microscopy techniques have been developed that circumvent the resolution limitation.
Approval Evidence
1 source1 linked approval claimfirst-pass slug super-resolution-fluorescence-microscopy
In recent years, however, a variety of super-resolution fluorescence microscopy techniques have been developed that circumvent the resolution limitation.
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technology summarysupports
Super-resolution fluorescence microscopy techniques have been developed to circumvent the resolution limitation of regular optical microscopy.
In recent years, however, a variety of super-resolution fluorescence microscopy techniques have been developed that circumvent the resolution limitation.
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Comparisons
Source-stated alternatives
The abstract contrasts these methods with regular optical microscopy and fluorescence microscopy operated under conventional diffraction-limited conditions.
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The abstract contrasts these methods with regular optical microscopy and fluorescence microscopy operated under conventional diffraction-limited conditions.
Source-backed strengths
enables nanoscale optical imaging beyond the diffraction limit
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enables nanoscale optical imaging beyond the diffraction limit
Compared with fluorescence microscopy
The abstract contrasts these methods with regular optical microscopy and fluorescence microscopy operated under conventional diffraction-limited conditions.
Shared frame: source-stated alternative in extracted literature
Strengths here: enables nanoscale optical imaging beyond the diffraction limit.
Relative tradeoffs: specific technique families and tradeoffs are not detailed in the abstract.
Source:
The abstract contrasts these methods with regular optical microscopy and fluorescence microscopy operated under conventional diffraction-limited conditions.
Compared with microscopy
The abstract contrasts these methods with regular optical microscopy and fluorescence microscopy operated under conventional diffraction-limited conditions.
Shared frame: source-stated alternative in extracted literature
Strengths here: enables nanoscale optical imaging beyond the diffraction limit.
Relative tradeoffs: specific technique families and tradeoffs are not detailed in the abstract.
Source:
The abstract contrasts these methods with regular optical microscopy and fluorescence microscopy operated under conventional diffraction-limited conditions.
Ranked Citations
- 1.