Source 1primary paper2003Journal of Spectroscopy
Toolkit/Surface plasmon resonance
Surface plasmon resonance
Also known as: SPR
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Surface plasmon resonance (SPR) ... makes it possible to measure accurately the adsorption of molecules on the metal surface and their eventual interactions with specific ligands.
Usefulness & Problems
Why this is useful
SPR detects refractive index changes near a metal surface to measure molecular adsorption and interactions with specific ligands. The abstract highlights real-time kinetic and label-free biosensing applications.; real-time measurement of ligand-receptor interaction kinetics; lead compound screening; DNA hybridization measurement; enzyme-substrate interaction measurement; polyclonal antibody characterization; epitope mapping; protein conformation studies; label-free immunoassays
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SPR detects refractive index changes near a metal surface to measure molecular adsorption and interactions with specific ligands. The abstract highlights real-time kinetic and label-free biosensing applications.
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real-time measurement of ligand-receptor interaction kinetics
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lead compound screening
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DNA hybridization measurement
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enzyme-substrate interaction measurement
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polyclonal antibody characterization
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epitope mapping
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protein conformation studies
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label-free immunoassays
Problem solved
It enables accurate, label-free, real-time measurement of biomolecular binding and related interaction kinetics across multiple biomedical assay types.; label-free detection of molecular adsorption and binding interactions; real-time monitoring of biomolecular association and dissociation
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It enables accurate, label-free, real-time measurement of biomolecular binding and related interaction kinetics across multiple biomedical assay types.
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label-free detection of molecular adsorption and binding interactions
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real-time monitoring of biomolecular association and dissociation
Problem links
label-free detection of molecular adsorption and binding interactions
LiteratureIt enables accurate, label-free, real-time measurement of biomolecular binding and related interaction kinetics across multiple biomedical assay types.
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It enables accurate, label-free, real-time measurement of biomolecular binding and related interaction kinetics across multiple biomedical assay types.
real-time monitoring of biomolecular association and dissociation
LiteratureIt enables accurate, label-free, real-time measurement of biomolecular binding and related interaction kinetics across multiple biomedical assay types.
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It enables accurate, label-free, real-time measurement of biomolecular binding and related interaction kinetics across multiple biomedical assay types.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
refractive index-based detectionsurface adsorption measurementsurface plasmon resonance sensingTarget processes
recombinationselectionInput: Chemical
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The method requires a metal surface, typically gold or silver, and in conventional formats specialized biosensing instruments and immobilized ligands or proteins on sensor chips.; requires metal surfaces such as gold or silver; conventional implementations use specialized biosensing instruments
The abstract notes that conventional SPR can be constrained by expensive chips, limited chip reuse, and complex immobilization chemistry.; conventional SPR uses expensive sensor chips with limited reuse capacity; conventional SPR requires complex chemistry for ligand or protein immobilization
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
SPR is applied to real-time measurement of ligand-receptor kinetics, lead compound screening, DNA hybridization, enzyme-substrate interactions, polyclonal antibody characterization, epitope mapping, protein conformation studies, and label-free immunoassays.
The technique is applied not only to the measurement in real‐time of the kinetics of ligand–receptor interactions and to the screening of lead compounds in the pharmaceutical industry, but also to the measurement of DNA hybridization, enzyme–substrate interactions, in polyclonal antibody characterization, epitope mapping, protein conformation studies and label‐free immunoassays.
Surface plasmon resonance enables accurate measurement of molecular adsorption on metal surfaces and interactions with specific ligands through sensitivity to refractive index changes near the surface.
Biomedical applications take advantage of the exquisite sensitivity of SPR to the refractive index of the medium next to the metal surface, which makes it possible to measure accurately the adsorption of molecules on the metal surface and their eventual interactions with specific ligands.
Conventional SPR relies on specialized biosensing instruments with expensive sensor chips of limited reuse capacity and requires complex chemistry for ligand or protein immobilization.
Conventional SPR is applied in specialized biosensing instruments. These instruments use expensive sensor chips of limited reuse capacity and require complex chemistry for ligand or protein immobilization.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug surface-plasmon-resonance
Surface plasmon resonance (SPR) ... makes it possible to measure accurately the adsorption of molecules on the metal surface and their eventual interactions with specific ligands.
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application scopesupports
SPR is applied to real-time measurement of ligand-receptor kinetics, lead compound screening, DNA hybridization, enzyme-substrate interactions, polyclonal antibody characterization, epitope mapping, protein conformation studies, and label-free immunoassays.
The technique is applied not only to the measurement in real‐time of the kinetics of ligand–receptor interactions and to the screening of lead compounds in the pharmaceutical industry, but also to the measurement of DNA hybridization, enzyme–substrate interactions, in polyclonal antibody characterization, epitope mapping, protein conformation studies and label‐free immunoassays.
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capabilitysupports
Surface plasmon resonance enables accurate measurement of molecular adsorption on metal surfaces and interactions with specific ligands through sensitivity to refractive index changes near the surface.
Biomedical applications take advantage of the exquisite sensitivity of SPR to the refractive index of the medium next to the metal surface, which makes it possible to measure accurately the adsorption of molecules on the metal surface and their eventual interactions with specific ligands.
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limitationsupports
Conventional SPR relies on specialized biosensing instruments with expensive sensor chips of limited reuse capacity and requires complex chemistry for ligand or protein immobilization.
Conventional SPR is applied in specialized biosensing instruments. These instruments use expensive sensor chips of limited reuse capacity and require complex chemistry for ligand or protein immobilization.
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Comparisons
Source-stated alternatives
The paper contrasts conventional instrument-based SPR with the authors' colloidal-gold SPR implementation monitored by UV-vis spectrophotometry.
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The paper contrasts conventional instrument-based SPR with the authors' colloidal-gold SPR implementation monitored by UV-vis spectrophotometry.
Source-backed strengths
exquisite sensitivity to refractive index changes near the metal surface; supports real-time kinetic measurements; label-free measurement modality
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exquisite sensitivity to refractive index changes near the metal surface
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supports real-time kinetic measurements
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label-free measurement modality
Surface plasmon resonance and high-throughput drug screening using hPSC-derived cellular models address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: chemical
Compared with ProKAS
Surface plasmon resonance and ProKAS address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: chemical
Compared with single-cell RNA sequencing
Surface plasmon resonance and single-cell RNA sequencing address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: chemical
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.