Toolkit/synaptoPAC

synaptoPAC

Multi-Component Switch·Research·Since 2020

Also known as: SynaptoPAC

Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

synaptoPAC is a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC developed as a light-responsive optogenetic tool for induction of presynaptic plasticity. It is reported to increase action potential-evoked transmission in some neuronal contexts, but this potentiation was not observed in non-granule hippocampal cultures or at Schaffer collateral synapses in CA1 acute slices.

Usefulness & Problems

Why this is useful

synaptoPAC is useful as a light-gated means to manipulate presynaptic cAMP signaling with subcellular targeting to presynaptic compartments. The available evidence indicates utility for probing presynaptic plasticity, while also showing that its functional effects are strongly dependent on neuronal and synaptic context.

Source:

In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3

Source:

In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission

Source:

In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission

Source:

Our work establishes synaptoPAC as an optogenetic tool that enables acute light-controlled potentiation of transmitter release at specific synapses in the brain

Problem solved

This tool addresses the need for an optogenetic method to induce presynaptic plasticity through presynaptically localized photoactivated adenylyl cyclase activity. The evidence specifically supports its use for testing whether elevating presynaptic cAMP is sufficient to enhance action potential-evoked transmission in selected preparations.

Source:

In order to establish optogenetic control of presynaptic cAMP levels and thereby presynaptic potentiation, we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC.

Source:

we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC

Taxonomy & Function

Primary hierarchy

Mechanism Branch

Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.

Techniques

No technique tags yet.

Target processes

recombination

Input: Light

Implementation Constraints

synaptoPAC is implemented as a presynaptically targeted form of the photoactivated adenylyl cyclase bPAC, indicating a fusion or targeting-based construct design. The supplied evidence supports light as the input modality and presynaptic localization as a design feature, but does not specify wavelengths, cofactors, expression systems, or delivery methods.

Its effects are context dependent: increased action potential-evoked transmission was not seen in hippocampal cultures of non-granule cells, and synaptoPAC activation did not trigger presynaptic potentiation at Schaffer collateral synapses in CA1 acute slices. The supplied evidence does not provide additional quantitative performance data, illumination parameters, or broader cross-system validation.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Observations

successMouseapplication demoMus musculus

Inferred from claim c5 during normalization. After light-triggered potentiation, mossy fiber transmission decreased within 20 minutes but remained enhanced after 30 minutes. Derived from claim c5. Quoted text: Following light-triggered potentiation, mossy fiber transmission decreased within 20 min, but remained enhanced still after 30 min.

Source:

persistent enhancement time30 mintime to partial decay20 min
mixedMouseapplication demoMus musculusC57BL/6Nacute brain slices

Inferred from claim c4 during normalization. In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered strong presynaptic potentiation at mossy fiber synapses in CA3 but not at Schaffer collateral synapses in CA1. Derived from claim c4. Quoted text: In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered a strong presynaptic potentiation at mossy fiber synapses in CA3, but not at Schaffer collateral synapses in CA1.

Source:

mixedMammalian Cell Lineapplication demo

Inferred from claim c3 during normalization. The increase in action potential-evoked transmission after synaptoPAC activation was not seen in hippocampal cultures of non-granule cells. Derived from claim c3. Quoted text: an effect not seen in hippocampal cultures of non-granule cells

Source:

successMammalian Cell Lineapplication demohippocampal granule cells

action potential-evoked transmission

Inferred from claim c2 during normalization. In cultures of hippocampal granule cells, blue-light activation of synaptoPAC increases action potential-evoked transmission. Derived from claim c2. Quoted text: In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission

Source:

successMouseapplication demoacute brain slices

Inferred from claim c4 during normalization. In acute brain slices, synaptoPAC activation immediately triggers strong presynaptic potentiation at mossy fiber terminals in CA3. Derived from claim c4. Quoted text: In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3

Source:

successMammalian Cell Lineapplication demoRattus norvegicusWistarhippocampal granule cells

Inferred from claim c2 during normalization. In cultures of hippocampal granule cells from Wistar rats, blue-light activation of synaptoPAC increased action potential-evoked transmission. Derived from claim c2. Quoted text: In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission

Source:

mixedMouseapplication demoMus musculusC57BL/6Nacute brain slices

Inferred from claim c4 during normalization. In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered strong presynaptic potentiation at mossy fiber synapses in CA3 but not at Schaffer collateral synapses in CA1. Derived from claim c4. Quoted text: In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered a strong presynaptic potentiation at mossy fiber synapses in CA3, but not at Schaffer collateral synapses in CA1.

Source:

successMammalian Cell Lineapplication demohippocampal granule cells

action potential-evoked transmission

Inferred from claim c2 during normalization. In cultures of hippocampal granule cells, blue-light activation of synaptoPAC increases action potential-evoked transmission. Derived from claim c2. Quoted text: In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission

Source:

successMouseapplication demoacute brain slices

Inferred from claim c4 during normalization. In acute brain slices, synaptoPAC activation immediately triggers strong presynaptic potentiation at mossy fiber terminals in CA3. Derived from claim c4. Quoted text: In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3

Source:

successMammalian Cell Lineapplication demoRattus norvegicusWistarhippocampal granule cells

Inferred from claim c2 during normalization. In cultures of hippocampal granule cells from Wistar rats, blue-light activation of synaptoPAC increased action potential-evoked transmission. Derived from claim c2. Quoted text: In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission

Source:

mixedMammalian Cell Lineapplication demo

Inferred from claim c3 during normalization. The increase in action potential-evoked transmission after synaptoPAC activation was not seen in hippocampal cultures of non-granule cells. Derived from claim c3. Quoted text: an effect not seen in hippocampal cultures of non-granule cells

Source:

mixedMouseapplication demoMus musculusC57BL/6Nacute brain slices

Inferred from claim c4 during normalization. In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered strong presynaptic potentiation at mossy fiber synapses in CA3 but not at Schaffer collateral synapses in CA1. Derived from claim c4. Quoted text: In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered a strong presynaptic potentiation at mossy fiber synapses in CA3, but not at Schaffer collateral synapses in CA1.

Source:

successMouseapplication demoMus musculus

Inferred from claim c5 during normalization. After light-triggered potentiation, mossy fiber transmission decreased within 20 minutes but remained enhanced after 30 minutes. Derived from claim c5. Quoted text: Following light-triggered potentiation, mossy fiber transmission decreased within 20 min, but remained enhanced still after 30 min.

Source:

persistent enhancement time30 mintime to partial decay20 min
successMammalian Cell Lineapplication demohippocampal granule cells

action potential-evoked transmission

Inferred from claim c2 during normalization. In cultures of hippocampal granule cells, blue-light activation of synaptoPAC increases action potential-evoked transmission. Derived from claim c2. Quoted text: In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission

Source:

successMouseapplication demoacute brain slices

Inferred from claim c4 during normalization. In acute brain slices, synaptoPAC activation immediately triggers strong presynaptic potentiation at mossy fiber terminals in CA3. Derived from claim c4. Quoted text: In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3

Source:

successMammalian Cell Lineapplication demoRattus norvegicusWistarhippocampal granule cells

Inferred from claim c2 during normalization. In cultures of hippocampal granule cells from Wistar rats, blue-light activation of synaptoPAC increased action potential-evoked transmission. Derived from claim c2. Quoted text: In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission

Source:

mixedMammalian Cell Lineapplication demo

Inferred from claim c3 during normalization. The increase in action potential-evoked transmission after synaptoPAC activation was not seen in hippocampal cultures of non-granule cells. Derived from claim c3. Quoted text: an effect not seen in hippocampal cultures of non-granule cells

Source:

mixedMouseapplication demoMus musculusC57BL/6Nacute brain slices

Inferred from claim c4 during normalization. In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered strong presynaptic potentiation at mossy fiber synapses in CA3 but not at Schaffer collateral synapses in CA1. Derived from claim c4. Quoted text: In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered a strong presynaptic potentiation at mossy fiber synapses in CA3, but not at Schaffer collateral synapses in CA1.

Source:

successMouseapplication demoMus musculus

Inferred from claim c5 during normalization. After light-triggered potentiation, mossy fiber transmission decreased within 20 minutes but remained enhanced after 30 minutes. Derived from claim c5. Quoted text: Following light-triggered potentiation, mossy fiber transmission decreased within 20 min, but remained enhanced still after 30 min.

Source:

persistent enhancement time30 mintime to partial decay20 min
successMammalian Cell Lineapplication demohippocampal granule cells

action potential-evoked transmission

Inferred from claim c2 during normalization. In cultures of hippocampal granule cells, blue-light activation of synaptoPAC increases action potential-evoked transmission. Derived from claim c2. Quoted text: In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission

Source:

successMouseapplication demoacute brain slices

Inferred from claim c4 during normalization. In acute brain slices, synaptoPAC activation immediately triggers strong presynaptic potentiation at mossy fiber terminals in CA3. Derived from claim c4. Quoted text: In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3

Source:

successMammalian Cell Lineapplication demoRattus norvegicusWistarhippocampal granule cells

Inferred from claim c2 during normalization. In cultures of hippocampal granule cells from Wistar rats, blue-light activation of synaptoPAC increased action potential-evoked transmission. Derived from claim c2. Quoted text: In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission

Source:

mixedMammalian Cell Lineapplication demo

Inferred from claim c3 during normalization. The increase in action potential-evoked transmission after synaptoPAC activation was not seen in hippocampal cultures of non-granule cells. Derived from claim c3. Quoted text: an effect not seen in hippocampal cultures of non-granule cells

Source:

mixedMouseapplication demoMus musculusC57BL/6Nacute brain slices

Inferred from claim c4 during normalization. In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered strong presynaptic potentiation at mossy fiber synapses in CA3 but not at Schaffer collateral synapses in CA1. Derived from claim c4. Quoted text: In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered a strong presynaptic potentiation at mossy fiber synapses in CA3, but not at Schaffer collateral synapses in CA1.

Source:

successMouseapplication demoMus musculus

Inferred from claim c5 during normalization. After light-triggered potentiation, mossy fiber transmission decreased within 20 minutes but remained enhanced after 30 minutes. Derived from claim c5. Quoted text: Following light-triggered potentiation, mossy fiber transmission decreased within 20 min, but remained enhanced still after 30 min.

Source:

persistent enhancement time30 mintime to partial decay20 min
successMammalian Cell Lineapplication demohippocampal granule cells

action potential-evoked transmission

Inferred from claim c2 during normalization. In cultures of hippocampal granule cells, blue-light activation of synaptoPAC increases action potential-evoked transmission. Derived from claim c2. Quoted text: In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission

Source:

successMouseapplication demoacute brain slices

Inferred from claim c4 during normalization. In acute brain slices, synaptoPAC activation immediately triggers strong presynaptic potentiation at mossy fiber terminals in CA3. Derived from claim c4. Quoted text: In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3

Source:

successMammalian Cell Lineapplication demoRattus norvegicusWistarhippocampal granule cells

Inferred from claim c2 during normalization. In cultures of hippocampal granule cells from Wistar rats, blue-light activation of synaptoPAC increased action potential-evoked transmission. Derived from claim c2. Quoted text: In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission

Source:

mixedMammalian Cell Lineapplication demo

Inferred from claim c3 during normalization. The increase in action potential-evoked transmission after synaptoPAC activation was not seen in hippocampal cultures of non-granule cells. Derived from claim c3. Quoted text: an effect not seen in hippocampal cultures of non-granule cells

Source:

mixedMouseapplication demoMus musculusC57BL/6Nacute brain slices

Inferred from claim c4 during normalization. In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered strong presynaptic potentiation at mossy fiber synapses in CA3 but not at Schaffer collateral synapses in CA1. Derived from claim c4. Quoted text: In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered a strong presynaptic potentiation at mossy fiber synapses in CA3, but not at Schaffer collateral synapses in CA1.

Source:

successMouseapplication demoMus musculus

Inferred from claim c5 during normalization. After light-triggered potentiation, mossy fiber transmission decreased within 20 minutes but remained enhanced after 30 minutes. Derived from claim c5. Quoted text: Following light-triggered potentiation, mossy fiber transmission decreased within 20 min, but remained enhanced still after 30 min.

Source:

persistent enhancement time30 mintime to partial decay20 min
successMammalian Cell Lineapplication demohippocampal granule cells

action potential-evoked transmission

Inferred from claim c2 during normalization. In cultures of hippocampal granule cells, blue-light activation of synaptoPAC increases action potential-evoked transmission. Derived from claim c2. Quoted text: In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission

Source:

successMouseapplication demoacute brain slices

Inferred from claim c4 during normalization. In acute brain slices, synaptoPAC activation immediately triggers strong presynaptic potentiation at mossy fiber terminals in CA3. Derived from claim c4. Quoted text: In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3

Source:

successMammalian Cell Lineapplication demoRattus norvegicusWistarhippocampal granule cells

Inferred from claim c2 during normalization. In cultures of hippocampal granule cells from Wistar rats, blue-light activation of synaptoPAC increased action potential-evoked transmission. Derived from claim c2. Quoted text: In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission

Source:

mixedMammalian Cell Lineapplication demo

Inferred from claim c3 during normalization. The increase in action potential-evoked transmission after synaptoPAC activation was not seen in hippocampal cultures of non-granule cells. Derived from claim c3. Quoted text: an effect not seen in hippocampal cultures of non-granule cells

Source:

mixedMouseapplication demoMus musculusC57BL/6Nacute brain slices

Inferred from claim c4 during normalization. In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered strong presynaptic potentiation at mossy fiber synapses in CA3 but not at Schaffer collateral synapses in CA1. Derived from claim c4. Quoted text: In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered a strong presynaptic potentiation at mossy fiber synapses in CA3, but not at Schaffer collateral synapses in CA1.

Source:

successMouseapplication demoMus musculus

Inferred from claim c5 during normalization. After light-triggered potentiation, mossy fiber transmission decreased within 20 minutes but remained enhanced after 30 minutes. Derived from claim c5. Quoted text: Following light-triggered potentiation, mossy fiber transmission decreased within 20 min, but remained enhanced still after 30 min.

Source:

persistent enhancement time30 mintime to partial decay20 min
successMouseapplication demoMus musculus

Inferred from claim c5 during normalization. After light-triggered potentiation, mossy fiber transmission decreased within 20 minutes but remained enhanced after 30 minutes. Derived from claim c5. Quoted text: Following light-triggered potentiation, mossy fiber transmission decreased within 20 min, but remained enhanced still after 30 min.

Source:

persistent enhancement time30 mintime to partial decay20 min
successMammalian Cell Lineapplication demohippocampal granule cells

action potential-evoked transmission

Inferred from claim c2 during normalization. In cultures of hippocampal granule cells, blue-light activation of synaptoPAC increases action potential-evoked transmission. Derived from claim c2. Quoted text: In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission

Source:

successMouseapplication demoacute brain slices

Inferred from claim c4 during normalization. In acute brain slices, synaptoPAC activation immediately triggers strong presynaptic potentiation at mossy fiber terminals in CA3. Derived from claim c4. Quoted text: In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3

Source:

successMammalian Cell Lineapplication demoRattus norvegicusWistarhippocampal granule cells

Inferred from claim c2 during normalization. In cultures of hippocampal granule cells from Wistar rats, blue-light activation of synaptoPAC increased action potential-evoked transmission. Derived from claim c2. Quoted text: In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission

Source:

mixedMammalian Cell Lineapplication demo

Inferred from claim c3 during normalization. The increase in action potential-evoked transmission after synaptoPAC activation was not seen in hippocampal cultures of non-granule cells. Derived from claim c3. Quoted text: an effect not seen in hippocampal cultures of non-granule cells

Source:

failedMammalian Cell Linefailed attempthippocampal non-granule cells

action potential-evoked transmission

Inferred from claim c3 during normalization. The increase in action potential-evoked transmission upon synaptoPAC activation was not seen in hippocampal cultures of non-granule cells. Derived from claim c3. Quoted text: an effect not seen in hippocampal cultures of non-granule cells

Source:

failedMammalian Cell Linefailed attempthippocampal non-granule cells

action potential-evoked transmission

Inferred from claim c3 during normalization. The increase in action potential-evoked transmission upon synaptoPAC activation was not seen in hippocampal cultures of non-granule cells. Derived from claim c3. Quoted text: an effect not seen in hippocampal cultures of non-granule cells

Source:

failedMammalian Cell Linefailed attempthippocampal non-granule cells

action potential-evoked transmission

Inferred from claim c3 during normalization. The increase in action potential-evoked transmission upon synaptoPAC activation was not seen in hippocampal cultures of non-granule cells. Derived from claim c3. Quoted text: an effect not seen in hippocampal cultures of non-granule cells

Source:

failedMammalian Cell Linefailed attempthippocampal non-granule cells

action potential-evoked transmission

Inferred from claim c3 during normalization. The increase in action potential-evoked transmission upon synaptoPAC activation was not seen in hippocampal cultures of non-granule cells. Derived from claim c3. Quoted text: an effect not seen in hippocampal cultures of non-granule cells

Source:

failedMammalian Cell Linefailed attempthippocampal non-granule cells

action potential-evoked transmission

Inferred from claim c3 during normalization. The increase in action potential-evoked transmission upon synaptoPAC activation was not seen in hippocampal cultures of non-granule cells. Derived from claim c3. Quoted text: an effect not seen in hippocampal cultures of non-granule cells

Source:

failedMammalian Cell Linefailed attempthippocampal non-granule cells

action potential-evoked transmission

Inferred from claim c3 during normalization. The increase in action potential-evoked transmission upon synaptoPAC activation was not seen in hippocampal cultures of non-granule cells. Derived from claim c3. Quoted text: an effect not seen in hippocampal cultures of non-granule cells

Source:

failedMammalian Cell Linefailed attempthippocampal non-granule cells

action potential-evoked transmission

Inferred from claim c3 during normalization. The increase in action potential-evoked transmission upon synaptoPAC activation was not seen in hippocampal cultures of non-granule cells. Derived from claim c3. Quoted text: an effect not seen in hippocampal cultures of non-granule cells

Source:

Supporting Sources

Ranked Claims

Claim 1cell type specificitysupports2020Source 2needs review

The increase in action potential-evoked transmission after synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells
Claim 2cell type specificitysupports2020Source 2needs review

The increase in action potential-evoked transmission after synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells
Claim 3cell type specificitysupports2020Source 2needs review

The increase in action potential-evoked transmission after synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells
Claim 4cell type specificitysupports2020Source 2needs review

The increase in action potential-evoked transmission after synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells
Claim 5cell type specificitysupports2020Source 2needs review

The increase in action potential-evoked transmission after synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells
Claim 6cell type specificitysupports2020Source 2needs review

The increase in action potential-evoked transmission after synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells
Claim 7cell type specificitysupports2020Source 2needs review

The increase in action potential-evoked transmission after synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells
Claim 8context specificitysupports2020Source 1needs review

In acute brain slices, synaptoPAC activation does not trigger presynaptic potentiation at Schaffer collateral synapses in CA1.

but not at Schaffer collateral synapse in CA1
Claim 9context specificitysupports2020Source 1needs review

In acute brain slices, synaptoPAC activation does not trigger presynaptic potentiation at Schaffer collateral synapses in CA1.

but not at Schaffer collateral synapse in CA1
Claim 10context specificitysupports2020Source 1needs review

In acute brain slices, synaptoPAC activation does not trigger presynaptic potentiation at Schaffer collateral synapses in CA1.

but not at Schaffer collateral synapse in CA1
Claim 11context specificitysupports2020Source 1needs review

In acute brain slices, synaptoPAC activation does not trigger presynaptic potentiation at Schaffer collateral synapses in CA1.

but not at Schaffer collateral synapse in CA1
Claim 12context specificitysupports2020Source 1needs review

In acute brain slices, synaptoPAC activation does not trigger presynaptic potentiation at Schaffer collateral synapses in CA1.

but not at Schaffer collateral synapse in CA1
Claim 13context specificitysupports2020Source 1needs review

In acute brain slices, synaptoPAC activation does not trigger presynaptic potentiation at Schaffer collateral synapses in CA1.

but not at Schaffer collateral synapse in CA1
Claim 14context specificitysupports2020Source 1needs review

In acute brain slices, synaptoPAC activation does not trigger presynaptic potentiation at Schaffer collateral synapses in CA1.

but not at Schaffer collateral synapse in CA1
Claim 15context specificitysupports2020Source 1needs review

The increase in action potential-evoked transmission upon synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells
Claim 16context specificitysupports2020Source 1needs review

The increase in action potential-evoked transmission upon synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells
Claim 17context specificitysupports2020Source 1needs review

The increase in action potential-evoked transmission upon synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells
Claim 18context specificitysupports2020Source 1needs review

The increase in action potential-evoked transmission upon synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells
Claim 19context specificitysupports2020Source 1needs review

The increase in action potential-evoked transmission upon synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells
Claim 20context specificitysupports2020Source 1needs review

The increase in action potential-evoked transmission upon synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells
Claim 21context specificitysupports2020Source 1needs review

The increase in action potential-evoked transmission upon synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells
Claim 22functional effectsupports2020Source 1needs review

In acute brain slices, synaptoPAC activation immediately triggers strong presynaptic potentiation at mossy fiber terminals in CA3.

In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3
Claim 23functional effectsupports2020Source 1needs review

In acute brain slices, synaptoPAC activation immediately triggers strong presynaptic potentiation at mossy fiber terminals in CA3.

In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3
Claim 24functional effectsupports2020Source 1needs review

In acute brain slices, synaptoPAC activation immediately triggers strong presynaptic potentiation at mossy fiber terminals in CA3.

In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3
Claim 25functional effectsupports2020Source 1needs review

In acute brain slices, synaptoPAC activation immediately triggers strong presynaptic potentiation at mossy fiber terminals in CA3.

In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3
Claim 26functional effectsupports2020Source 1needs review

In acute brain slices, synaptoPAC activation immediately triggers strong presynaptic potentiation at mossy fiber terminals in CA3.

In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3
Claim 27functional effectsupports2020Source 1needs review

In acute brain slices, synaptoPAC activation immediately triggers strong presynaptic potentiation at mossy fiber terminals in CA3.

In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3
Claim 28functional effectsupports2020Source 1needs review

In acute brain slices, synaptoPAC activation immediately triggers strong presynaptic potentiation at mossy fiber terminals in CA3.

In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3
Claim 29functional effectsupports2020Source 1needs review

In cultures of hippocampal granule cells, blue-light activation of synaptoPAC increases action potential-evoked transmission.

In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission
Claim 30functional effectsupports2020Source 1needs review

In cultures of hippocampal granule cells, blue-light activation of synaptoPAC increases action potential-evoked transmission.

In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission
Claim 31functional effectsupports2020Source 1needs review

In cultures of hippocampal granule cells, blue-light activation of synaptoPAC increases action potential-evoked transmission.

In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission
Claim 32functional effectsupports2020Source 1needs review

In cultures of hippocampal granule cells, blue-light activation of synaptoPAC increases action potential-evoked transmission.

In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission
Claim 33functional effectsupports2020Source 1needs review

In cultures of hippocampal granule cells, blue-light activation of synaptoPAC increases action potential-evoked transmission.

In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission
Claim 34functional effectsupports2020Source 1needs review

In cultures of hippocampal granule cells, blue-light activation of synaptoPAC increases action potential-evoked transmission.

In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission
Claim 35functional effectsupports2020Source 1needs review

In cultures of hippocampal granule cells, blue-light activation of synaptoPAC increases action potential-evoked transmission.

In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission
Claim 36functional effectsupports2020Source 2needs review

In cultures of hippocampal granule cells from Wistar rats, blue-light activation of synaptoPAC increased action potential-evoked transmission.

In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission
Claim 37functional effectsupports2020Source 2needs review

In cultures of hippocampal granule cells from Wistar rats, blue-light activation of synaptoPAC increased action potential-evoked transmission.

In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission
Claim 38functional effectsupports2020Source 2needs review

In cultures of hippocampal granule cells from Wistar rats, blue-light activation of synaptoPAC increased action potential-evoked transmission.

In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission
Claim 39functional effectsupports2020Source 2needs review

In cultures of hippocampal granule cells from Wistar rats, blue-light activation of synaptoPAC increased action potential-evoked transmission.

In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission
Claim 40functional effectsupports2020Source 2needs review

In cultures of hippocampal granule cells from Wistar rats, blue-light activation of synaptoPAC increased action potential-evoked transmission.

In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission
Claim 41functional effectsupports2020Source 2needs review

In cultures of hippocampal granule cells from Wistar rats, blue-light activation of synaptoPAC increased action potential-evoked transmission.

In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission
Claim 42functional effectsupports2020Source 2needs review

In cultures of hippocampal granule cells from Wistar rats, blue-light activation of synaptoPAC increased action potential-evoked transmission.

In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission
Claim 43noveltysupports2020Source 1needs review

SynaptoPAC is presented as the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain.

SynaptoPAC is the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain
Claim 44noveltysupports2020Source 1needs review

SynaptoPAC is presented as the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain.

SynaptoPAC is the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain
Claim 45noveltysupports2020Source 1needs review

SynaptoPAC is presented as the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain.

SynaptoPAC is the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain
Claim 46noveltysupports2020Source 1needs review

SynaptoPAC is presented as the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain.

SynaptoPAC is the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain
Claim 47noveltysupports2020Source 1needs review

SynaptoPAC is presented as the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain.

SynaptoPAC is the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain
Claim 48noveltysupports2020Source 1needs review

SynaptoPAC is presented as the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain.

SynaptoPAC is the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain
Claim 49noveltysupports2020Source 1needs review

SynaptoPAC is presented as the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain.

SynaptoPAC is the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain
Claim 50phenotypic similaritysupports2020Source 1needs review

Optogenetic potentiation by synaptoPAC alters short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

Optogenetic potentiation alters the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.
Claim 51phenotypic similaritysupports2020Source 1needs review

Optogenetic potentiation by synaptoPAC alters short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

Optogenetic potentiation alters the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.
Claim 52phenotypic similaritysupports2020Source 1needs review

Optogenetic potentiation by synaptoPAC alters short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

Optogenetic potentiation alters the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.
Claim 53phenotypic similaritysupports2020Source 1needs review

Optogenetic potentiation by synaptoPAC alters short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

Optogenetic potentiation alters the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.
Claim 54phenotypic similaritysupports2020Source 1needs review

Optogenetic potentiation by synaptoPAC alters short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

Optogenetic potentiation alters the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.
Claim 55phenotypic similaritysupports2020Source 1needs review

Optogenetic potentiation by synaptoPAC alters short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

Optogenetic potentiation alters the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.
Claim 56phenotypic similaritysupports2020Source 1needs review

Optogenetic potentiation by synaptoPAC alters short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

Optogenetic potentiation alters the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.
Claim 57plasticity signaturesupports2020Source 2needs review

Optogenetic potentiation by synaptoPAC altered short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

The optogenetic potentiation altered the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.
Claim 58plasticity signaturesupports2020Source 2needs review

Optogenetic potentiation by synaptoPAC altered short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

The optogenetic potentiation altered the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.
Claim 59plasticity signaturesupports2020Source 2needs review

Optogenetic potentiation by synaptoPAC altered short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

The optogenetic potentiation altered the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.
Claim 60plasticity signaturesupports2020Source 2needs review

Optogenetic potentiation by synaptoPAC altered short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

The optogenetic potentiation altered the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.
Claim 61plasticity signaturesupports2020Source 2needs review

Optogenetic potentiation by synaptoPAC altered short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

The optogenetic potentiation altered the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.
Claim 62plasticity signaturesupports2020Source 2needs review

Optogenetic potentiation by synaptoPAC altered short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

The optogenetic potentiation altered the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.
Claim 63plasticity signaturesupports2020Source 2needs review

Optogenetic potentiation by synaptoPAC altered short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

The optogenetic potentiation altered the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.
Claim 64synapse specific effectsupports2020Source 2needs review

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered strong presynaptic potentiation at mossy fiber synapses in CA3 but not at Schaffer collateral synapses in CA1.

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered a strong presynaptic potentiation at mossy fiber synapses in CA3, but not at Schaffer collateral synapses in CA1.
Claim 65synapse specific effectsupports2020Source 2needs review

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered strong presynaptic potentiation at mossy fiber synapses in CA3 but not at Schaffer collateral synapses in CA1.

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered a strong presynaptic potentiation at mossy fiber synapses in CA3, but not at Schaffer collateral synapses in CA1.
Claim 66synapse specific effectsupports2020Source 2needs review

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered strong presynaptic potentiation at mossy fiber synapses in CA3 but not at Schaffer collateral synapses in CA1.

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered a strong presynaptic potentiation at mossy fiber synapses in CA3, but not at Schaffer collateral synapses in CA1.
Claim 67synapse specific effectsupports2020Source 2needs review

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered strong presynaptic potentiation at mossy fiber synapses in CA3 but not at Schaffer collateral synapses in CA1.

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered a strong presynaptic potentiation at mossy fiber synapses in CA3, but not at Schaffer collateral synapses in CA1.
Claim 68synapse specific effectsupports2020Source 2needs review

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered strong presynaptic potentiation at mossy fiber synapses in CA3 but not at Schaffer collateral synapses in CA1.

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered a strong presynaptic potentiation at mossy fiber synapses in CA3, but not at Schaffer collateral synapses in CA1.
Claim 69synapse specific effectsupports2020Source 2needs review

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered strong presynaptic potentiation at mossy fiber synapses in CA3 but not at Schaffer collateral synapses in CA1.

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered a strong presynaptic potentiation at mossy fiber synapses in CA3, but not at Schaffer collateral synapses in CA1.
Claim 70synapse specific effectsupports2020Source 2needs review

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered strong presynaptic potentiation at mossy fiber synapses in CA3 but not at Schaffer collateral synapses in CA1.

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered a strong presynaptic potentiation at mossy fiber synapses in CA3, but not at Schaffer collateral synapses in CA1.
Claim 71temporal dynamicssupports2020Source 2needs review

After light-triggered potentiation, mossy fiber transmission decreased within 20 minutes but remained enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreased within 20 min, but remained enhanced still after 30 min.
persistent enhancement time 30 mintime to partial decay 20 min
Claim 72temporal dynamicssupports2020Source 2needs review

After light-triggered potentiation, mossy fiber transmission decreased within 20 minutes but remained enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreased within 20 min, but remained enhanced still after 30 min.
persistent enhancement time 30 mintime to partial decay 20 min
Claim 73temporal dynamicssupports2020Source 2needs review

After light-triggered potentiation, mossy fiber transmission decreased within 20 minutes but remained enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreased within 20 min, but remained enhanced still after 30 min.
persistent enhancement time 30 mintime to partial decay 20 min
Claim 74temporal dynamicssupports2020Source 2needs review

After light-triggered potentiation, mossy fiber transmission decreased within 20 minutes but remained enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreased within 20 min, but remained enhanced still after 30 min.
persistent enhancement time 30 mintime to partial decay 20 min
Claim 75temporal dynamicssupports2020Source 2needs review

After light-triggered potentiation, mossy fiber transmission decreased within 20 minutes but remained enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreased within 20 min, but remained enhanced still after 30 min.
persistent enhancement time 30 mintime to partial decay 20 min
Claim 76temporal dynamicssupports2020Source 2needs review

After light-triggered potentiation, mossy fiber transmission decreased within 20 minutes but remained enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreased within 20 min, but remained enhanced still after 30 min.
persistent enhancement time 30 mintime to partial decay 20 min
Claim 77temporal dynamicssupports2020Source 2needs review

After light-triggered potentiation, mossy fiber transmission decreased within 20 minutes but remained enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreased within 20 min, but remained enhanced still after 30 min.
persistent enhancement time 30 mintime to partial decay 20 min
Claim 78temporal dynamicssupports2020Source 1needs review

After light-triggered potentiation, mossy fiber transmission decreases within 20 minutes but remains enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreases within 20 minutes, but remains enhanced still after 30 min.
enhancement persists at 30 mintime to partial decrease 20 min
Claim 79temporal dynamicssupports2020Source 1needs review

After light-triggered potentiation, mossy fiber transmission decreases within 20 minutes but remains enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreases within 20 minutes, but remains enhanced still after 30 min.
enhancement persists at 30 mintime to partial decrease 20 min
Claim 80temporal dynamicssupports2020Source 1needs review

After light-triggered potentiation, mossy fiber transmission decreases within 20 minutes but remains enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreases within 20 minutes, but remains enhanced still after 30 min.
enhancement persists at 30 mintime to partial decrease 20 min
Claim 81temporal dynamicssupports2020Source 1needs review

After light-triggered potentiation, mossy fiber transmission decreases within 20 minutes but remains enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreases within 20 minutes, but remains enhanced still after 30 min.
enhancement persists at 30 mintime to partial decrease 20 min
Claim 82temporal dynamicssupports2020Source 1needs review

After light-triggered potentiation, mossy fiber transmission decreases within 20 minutes but remains enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreases within 20 minutes, but remains enhanced still after 30 min.
enhancement persists at 30 mintime to partial decrease 20 min
Claim 83temporal dynamicssupports2020Source 1needs review

After light-triggered potentiation, mossy fiber transmission decreases within 20 minutes but remains enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreases within 20 minutes, but remains enhanced still after 30 min.
enhancement persists at 30 mintime to partial decrease 20 min
Claim 84temporal dynamicssupports2020Source 1needs review

After light-triggered potentiation, mossy fiber transmission decreases within 20 minutes but remains enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreases within 20 minutes, but remains enhanced still after 30 min.
enhancement persists at 30 mintime to partial decrease 20 min
Claim 85tool capabilitysupports2020Source 2needs review

SynaptoPAC enables acute light-controlled potentiation of transmitter release at specific synapses in the brain.

Our work establishes synaptoPAC as an optogenetic tool that enables acute light-controlled potentiation of transmitter release at specific synapses in the brain
Claim 86tool capabilitysupports2020Source 2needs review

SynaptoPAC enables acute light-controlled potentiation of transmitter release at specific synapses in the brain.

Our work establishes synaptoPAC as an optogenetic tool that enables acute light-controlled potentiation of transmitter release at specific synapses in the brain
Claim 87tool capabilitysupports2020Source 2needs review

SynaptoPAC enables acute light-controlled potentiation of transmitter release at specific synapses in the brain.

Our work establishes synaptoPAC as an optogenetic tool that enables acute light-controlled potentiation of transmitter release at specific synapses in the brain
Claim 88tool capabilitysupports2020Source 2needs review

SynaptoPAC enables acute light-controlled potentiation of transmitter release at specific synapses in the brain.

Our work establishes synaptoPAC as an optogenetic tool that enables acute light-controlled potentiation of transmitter release at specific synapses in the brain
Claim 89tool capabilitysupports2020Source 2needs review

SynaptoPAC enables acute light-controlled potentiation of transmitter release at specific synapses in the brain.

Our work establishes synaptoPAC as an optogenetic tool that enables acute light-controlled potentiation of transmitter release at specific synapses in the brain
Claim 90tool capabilitysupports2020Source 2needs review

SynaptoPAC enables acute light-controlled potentiation of transmitter release at specific synapses in the brain.

Our work establishes synaptoPAC as an optogenetic tool that enables acute light-controlled potentiation of transmitter release at specific synapses in the brain
Claim 91tool capabilitysupports2020Source 2needs review

SynaptoPAC enables acute light-controlled potentiation of transmitter release at specific synapses in the brain.

Our work establishes synaptoPAC as an optogenetic tool that enables acute light-controlled potentiation of transmitter release at specific synapses in the brain
Claim 92tool developmentsupports2020Source 1needs review

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC
Claim 93tool developmentsupports2020Source 2needs review

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

In order to establish optogenetic control of presynaptic cAMP levels and thereby presynaptic potentiation, we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC.
Claim 94tool developmentsupports2020Source 2needs review

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

In order to establish optogenetic control of presynaptic cAMP levels and thereby presynaptic potentiation, we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC.
Claim 95tool developmentsupports2020Source 2needs review

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

In order to establish optogenetic control of presynaptic cAMP levels and thereby presynaptic potentiation, we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC.
Claim 96tool developmentsupports2020Source 1needs review

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC
Claim 97tool developmentsupports2020Source 2needs review

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

In order to establish optogenetic control of presynaptic cAMP levels and thereby presynaptic potentiation, we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC.
Claim 98tool developmentsupports2020Source 2needs review

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

In order to establish optogenetic control of presynaptic cAMP levels and thereby presynaptic potentiation, we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC.
Claim 99tool developmentsupports2020Source 1needs review

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC
Claim 100tool developmentsupports2020Source 1needs review

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC
Claim 101tool developmentsupports2020Source 1needs review

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC
Claim 102tool developmentsupports2020Source 1needs review

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC
Claim 103tool developmentsupports2020Source 2needs review

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

In order to establish optogenetic control of presynaptic cAMP levels and thereby presynaptic potentiation, we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC.
Claim 104tool developmentsupports2020Source 1needs review

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC
Claim 105tool developmentsupports2020Source 2needs review

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

In order to establish optogenetic control of presynaptic cAMP levels and thereby presynaptic potentiation, we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC.

Approval Evidence

2 sources15 linked approval claimsfirst-pass slug synaptopac
we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC

Source:

we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC

Source:

cell type specificitysupports

The increase in action potential-evoked transmission after synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells

Source:

context specificitysupports

In acute brain slices, synaptoPAC activation does not trigger presynaptic potentiation at Schaffer collateral synapses in CA1.

but not at Schaffer collateral synapse in CA1

Source:

context specificitysupports

The increase in action potential-evoked transmission upon synaptoPAC activation was not seen in hippocampal cultures of non-granule cells.

an effect not seen in hippocampal cultures of non-granule cells

Source:

functional effectsupports

In acute brain slices, synaptoPAC activation immediately triggers strong presynaptic potentiation at mossy fiber terminals in CA3.

In acute brain slices, synaptoPAC activation immediately triggers a strong presynaptic potentiation at mossy fiber terminals in CA3

Source:

functional effectsupports

In cultures of hippocampal granule cells, blue-light activation of synaptoPAC increases action potential-evoked transmission.

In cultures of hippocampal granule cells, activation of synaptoPAC with blue light increases action potential-evoked transmission

Source:

functional effectsupports

In cultures of hippocampal granule cells from Wistar rats, blue-light activation of synaptoPAC increased action potential-evoked transmission.

In cultures of hippocampal granule cells of Wistar rats, activation of synaptoPAC with blue light increased action potential-evoked transmission

Source:

noveltysupports

SynaptoPAC is presented as the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain.

SynaptoPAC is the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain

Source:

phenotypic similaritysupports

Optogenetic potentiation by synaptoPAC alters short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

Optogenetic potentiation alters the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.

Source:

plasticity signaturesupports

Optogenetic potentiation by synaptoPAC altered short-term plasticity dynamics of release in a manner reminiscent of presynaptic LTP.

The optogenetic potentiation altered the short-term plasticity dynamics of release, reminiscent of presynaptic LTP.

Source:

synapse specific effectsupports

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered strong presynaptic potentiation at mossy fiber synapses in CA3 but not at Schaffer collateral synapses in CA1.

In acute brain slices of C57BL/6N mice, synaptoPAC activation immediately triggered a strong presynaptic potentiation at mossy fiber synapses in CA3, but not at Schaffer collateral synapses in CA1.

Source:

temporal dynamicssupports

After light-triggered potentiation, mossy fiber transmission decreased within 20 minutes but remained enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreased within 20 min, but remained enhanced still after 30 min.

Source:

temporal dynamicssupports

After light-triggered potentiation, mossy fiber transmission decreases within 20 minutes but remains enhanced after 30 minutes.

Following light-triggered potentiation, mossy fiber transmission decreases within 20 minutes, but remains enhanced still after 30 min.

Source:

tool capabilitysupports

SynaptoPAC enables acute light-controlled potentiation of transmitter release at specific synapses in the brain.

Our work establishes synaptoPAC as an optogenetic tool that enables acute light-controlled potentiation of transmitter release at specific synapses in the brain

Source:

tool developmentsupports

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

In order to establish optogenetic control of presynaptic cAMP levels and thereby presynaptic potentiation, we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC.

Source:

tool developmentsupports

SynaptoPAC was developed as a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC to enable optogenetic control of presynaptic cAMP levels and presynaptic potentiation.

we developed synaptoPAC, a presynaptically targeted version of the photoactivated adenylyl cyclase bPAC

Source:

Comparisons

Source-backed strengths

A key strength is that synaptoPAC combines light responsiveness with presynaptic targeting by using a presynaptically targeted version of bPAC. It was reported to increase action potential-evoked transmission in some neuronal contexts, supporting its intended function as an inducer of presynaptic plasticity.

Source:

SynaptoPAC is the first optogenetic tool that allows acute light-controlled potentiation of transmitter release at specific synapses of the brain

Ranked Citations

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    StructuralSource 12020Claim 8Claim 9Claim 10

    Seeded from load plan for claim c8. Extracted from this source document.

  2. 2.
    StructuralSource 2Journal of Neurochemistry2020Claim 1Claim 2Claim 3

    Seeded from load plan for claim c7. Extracted from this source document.