Source 1primary paper2023Biophysical Journal
Toolkit/time-resolved EPR
time-resolved EPR
Also known as: time-resolved EPR
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Time-resolved EPR is an assay method used to investigate light-triggered functional dynamics in the AsLOV2 photosensory domain at high magnetic fields. The supplied evidence supports its use as a light-responsive biophysical readout for AsLOV2.
Usefulness & Problems
Why this is useful
This method is useful for probing protein dynamics that are triggered by light in a photosensory domain. In the supplied evidence, its demonstrated utility is specifically the study of AsLOV2 functional dynamics under high-field EPR conditions.
Problem solved
It addresses the problem of measuring light-induced functional dynamics in the AsLOV2 domain with a time-resolved magnetic resonance assay. The evidence does not provide broader benchmarking against other dynamic measurement methods.
Problem links
Need precise spatiotemporal control with light input
DerivedTime-resolved EPR is an assay method used to study light-triggered functional dynamics in the AsLOV2 photosensory domain at high magnetic fields. The supplied evidence specifically supports its application to probing light-induced behavior of AsLOV2.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
electron paramagnetic resonance detectionelectron paramagnetic resonance detectionlight-triggered protein dynamics measurementlight-triggered protein dynamics measurementTechniques
Functional AssayTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The method is applied with light as the input modality and uses high magnetic field EPR to monitor AsLOV2 dynamics. The supplied evidence does not specify sample preparation, labeling requirements, cofactors, expression system, or instrument settings.
The supplied evidence is limited to a study focus statement and does not report temporal resolution, sensitivity, spin-labeling strategy, or comparative performance. Validation is only explicitly supported for AsLOV2, so generalizability to other proteins or LOV domains is not established here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2004Journal of the American Chemical Society
Ranked Claims
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
Approval Evidence
2 sources1 linked approval claimfirst-pass slugs time-resolved-electron-paramagnetic-resonance, time-resolved-epr
Light triggered functional dynamics of AsLOV2 by time-resolved EPR at high magnetic fields
Source:
studied by time-resolved electron paramagnetic resonance (EPR)
Source:
study focussupports
The paper studies light-triggered functional dynamics of AsLOV2 using time-resolved EPR at high magnetic fields.
Source:
Comparisons
Source-backed strengths
The evidence indicates that the method can resolve light-triggered functional dynamics and that it is implemented at high magnetic fields. This supports its value as a specialized biophysical assay for photoactivated behavior in AsLOV2.
Compared with electron paramagnetic resonance
time-resolved EPR and electron paramagnetic resonance address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: electron paramagnetic resonance detection; same primary input modality: light
Compared with native green gel system
time-resolved EPR and native green gel system address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with plant transcriptome profiling
time-resolved EPR and plant transcriptome profiling address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
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Extracted from this source document.