Source 1primary paper2021Nature Communications
Toolkit/translational titration
translational titration
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Translational titration is an application of genetic code expansion in Bacillus subtilis that modulates protein production at the level of translation. In the cited study, it was implemented within a broad and efficient noncanonical amino acid incorporation platform that also supported click-labelling and photo-crosslinking.
Usefulness & Problems
Why this is useful
This approach is useful for precise in vivo control of protein output in B. subtilis through translation-level modulation. The study used these tools to begin interrogating bacterial cytokinesis by precisely modulating cell division dynamics.
Source:
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
we demonstrate broad and efficient genetic code expansion in B. subtilis by incorporating 20 distinct non-standard amino acids within proteins using 3 different families of genetic code expansion systems and two choices of codons
Problem solved
It addresses the problem of tuning protein production in B. subtilis with the same genetic code expansion framework used for other noncanonical amino acid-enabled functions. The cited work specifically positions it as a way to modulate translation for studying cell division behavior in vivo.
Source:
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Problem links
Need precise spatiotemporal control with light input
DerivedTranslational titration is an application of genetic code expansion in Bacillus subtilis that modulates protein production at the level of translation. In the cited study, it was implemented within a broad and efficient noncanonical amino acid incorporation platform that also supported click-labelling and photo-crosslinking.
Need tighter control over protein production
DerivedTranslational titration is an application of genetic code expansion in Bacillus subtilis that modulates protein production at the level of translation. In the cited study, it was implemented within a broad and efficient noncanonical amino acid incorporation platform that also supported click-labelling and photo-crosslinking.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
translationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: spectral hardware requirementoperating role: builder
Implementation depends on genetic code expansion systems in Bacillus subtilis, and the source study reports three system families and two codon choices. The provided evidence does not specify the noncanonical amino acids, orthogonal translation components, construct architecture, or any light-dependent implementation details for translational titration.
The supplied evidence does not report quantitative performance metrics, dynamic range, target proteins, or comparative benchmarks for translational titration itself. The evidence is limited to a single source study in B. subtilis, so generality across organisms and applications is not established here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successBacteriaapplication demoBacillus subtilis
Inferred from claim c2 during normalization. The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration. Derived from claim c2. Quoted text: We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
successBacteriaapplication demo
Inferred from claim c5 during normalization. These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo. Derived from claim c5. Quoted text: begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
Source:
successBacteriaapplication demoBacillus subtilis
Inferred from claim c2 during normalization. The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration. Derived from claim c2. Quoted text: We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
successBacteriaapplication demo
Inferred from claim c5 during normalization. These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo. Derived from claim c5. Quoted text: begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
Source:
successBacteriaapplication demoBacillus subtilis
Inferred from claim c2 during normalization. The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration. Derived from claim c2. Quoted text: We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
successBacteriaapplication demo
Inferred from claim c5 during normalization. These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo. Derived from claim c5. Quoted text: begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
Source:
successBacteriaapplication demoBacillus subtilis
Inferred from claim c2 during normalization. The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration. Derived from claim c2. Quoted text: We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
successBacteriaapplication demo
Inferred from claim c5 during normalization. These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo. Derived from claim c5. Quoted text: begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
Source:
successBacteriaapplication demoBacillus subtilis
Inferred from claim c2 during normalization. The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration. Derived from claim c2. Quoted text: We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
successBacteriaapplication demo
Inferred from claim c5 during normalization. These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo. Derived from claim c5. Quoted text: begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
Source:
successBacteriaapplication demo
Inferred from claim c5 during normalization. These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo. Derived from claim c5. Quoted text: begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
Source:
successBacteriaapplication demoBacillus subtilis
Inferred from claim c2 during normalization. The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration. Derived from claim c2. Quoted text: We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
successBacteriaapplication demoBacillus subtilis
Inferred from claim c2 during normalization. The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration. Derived from claim c2. Quoted text: We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
successBacteriaapplication demo
Inferred from claim c5 during normalization. These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo. Derived from claim c5. Quoted text: begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
Source:
Supporting Sources
Ranked Claims
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
The authors demonstrate broad and efficient genetic code expansion in Bacillus subtilis using 3 families of genetic code expansion systems and 2 codon choices.
we demonstrate broad and efficient genetic code expansion in B. subtilis by incorporating 20 distinct non-standard amino acids within proteins using 3 different families of genetic code expansion systems and two choices of codons
codon choices 2distinct non-standard amino acids incorporated 20genetic code expansion system families 3
These tools allowed the authors to demonstrate differences between E. coli and Bacillus subtilis stop codon suppression.
These tools allow us to demonstrate differences between E. coli and B. subtilis stop codon suppression
The paper concerns designing efficient genetic code expansion in Bacillus subtilis to gain biological insights.
Designing efficient genetic code expansion in Bacillus subtilis to gain biological insights
Section: title
These tools were used to validate a predicted protein-protein binding interface.
validate a predicted protein-protein binding interface
These tools were used to validate a predicted protein-protein binding interface.
validate a predicted protein-protein binding interface
These tools were used to validate a predicted protein-protein binding interface.
validate a predicted protein-protein binding interface
These tools were used to validate a predicted protein-protein binding interface.
validate a predicted protein-protein binding interface
These tools were used to validate a predicted protein-protein binding interface.
validate a predicted protein-protein binding interface
These tools were used to validate a predicted protein-protein binding interface.
validate a predicted protein-protein binding interface
These tools were used to validate a predicted protein-protein binding interface.
validate a predicted protein-protein binding interface
Approval Evidence
1 source2 linked approval claimsfirst-pass slug translational-titration
We use these systems to achieve ... translational titration
Source:
applicationsupports
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
biological applicationsupports
These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
Source:
Comparisons
Source-backed strengths
The method was demonstrated in the context of broad and efficient genetic code expansion in B. subtilis, using three families of genetic code expansion systems and two codon choices. Its inclusion alongside click-labelling and photo-crosslinking indicates that translational titration can be integrated into a multifunctional noncanonical amino acid platform.
Source:
These tools allow us to demonstrate differences between E. coli and B. subtilis stop codon suppression
Compared with brain stimulation
translational titration and brain stimulation address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control; same primary input modality: light
Strengths here: looks easier to implement in practice.
Compared with click-labelling
translational titration and click-labelling address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: genetic code expansion, translation control, translation_control; same primary input modality: light
Strengths here: looks easier to implement in practice.
Compared with thermal sonogenetics
translational titration and thermal sonogenetics address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control; same primary input modality: light
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.