Source 1primary paper2025MED
Toolkit/two new acoustic reporter genes
two new acoustic reporter genes
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Here we use rational protein design and directed evolution to develop two new ARGs that can be distinguished from each other based on their acoustic pressure-response profiles, enabling 'two-tone' ultrasound imaging of gene expression.
Usefulness & Problems
Why this is useful
These engineered ARG variants provide two distinguishable ultrasound reporter signals based on different acoustic pressure-response profiles.; two-tone ultrasound imaging of gene expression; multiplexed imaging of cellular states or populations
Source:
These engineered ARG variants provide two distinguishable ultrasound reporter signals based on different acoustic pressure-response profiles.
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two-tone ultrasound imaging of gene expression
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multiplexed imaging of cellular states or populations
Problem solved
They address the inability of earlier ARGs to support multiplexed imaging of different cell states or populations.; overcomes the single-sound limitation of prior ARGs
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They address the inability of earlier ARGs to support multiplexed imaging of different cell states or populations.
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overcomes the single-sound limitation of prior ARGs
Problem links
overcomes the single-sound limitation of prior ARGs
LiteratureThey address the inability of earlier ARGs to support multiplexed imaging of different cell states or populations.
Source:
They address the inability of earlier ARGs to support multiplexed imaging of different cell states or populations.
Published Workflows
Objective: Develop multiplexable acoustic reporter genes for two-tone ultrasound imaging of gene expression and demonstrate their use for distinguishing cell populations and states in vitro and in vivo.
Why it works: The workflow is presented as combining rational protein design and directed evolution to create reporter variants with distinct acoustic pressure-response profiles, which then enables two-tone ultrasound imaging.
acoustic discrimination based on pressure-response profilesrational protein designdirected evolutionultrasound imaging
Stages
- 1.Reporter engineering(library_design)
To overcome the single-sound limitation of prior ARGs by creating two distinguishable reporter variants.
Selection: Develop new ARG variants with distinguishable acoustic pressure-response profiles.
- 2.In vitro utility demonstration(functional_characterization)
To show that the engineered reporters are useful for distinguishing biological populations before in vivo application.
Selection: Assess whether multiplexed ARGs can delineate bacterial cell species and cell states in vitro.
- 3.In vivo application(in_vivo_validation)
To demonstrate that multiplexed ARG imaging works in relevant in vivo settings including the mouse gastrointestinal tract and tumor-colonizing bacterial agents.
Selection: Apply multiplexed ARGs to image distinct bacterial subpopulations in living mice.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: reporter
The abstract supports that they were created using rational protein design and directed evolution and require ultrasound-based pressure-response discrimination for use.; requires pressure-response-based acoustic discrimination
Prior acoustic reporter genes were limited to a single acoustic signature, preventing multiplexed imaging of cellular states or populations.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Multiplexed acoustic reporter genes were used to delineate bacterial cell species and cell states in vitro and to image distinct subpopulations of probiotics in the mouse gastrointestinal tract and tumor-colonizing bacterial agents in vivo.
Acoustic reporter genes enable ultrasound imaging of gene expression with high-resolution access to deep, optically opaque living tissues.
Rational protein design and directed evolution produced two new acoustic reporter genes distinguishable by acoustic pressure-response profiles, enabling two-tone ultrasound imaging of gene expression.
Prior acoustic reporter genes were limited to a single acoustic signature, preventing multiplexed imaging of cellular states or populations.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug two-new-acoustic-reporter-genes
Here we use rational protein design and directed evolution to develop two new ARGs that can be distinguished from each other based on their acoustic pressure-response profiles, enabling 'two-tone' ultrasound imaging of gene expression.
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applicationsupports
Multiplexed acoustic reporter genes were used to delineate bacterial cell species and cell states in vitro and to image distinct subpopulations of probiotics in the mouse gastrointestinal tract and tumor-colonizing bacterial agents in vivo.
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engineering outcomesupports
Rational protein design and directed evolution produced two new acoustic reporter genes distinguishable by acoustic pressure-response profiles, enabling two-tone ultrasound imaging of gene expression.
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Comparisons
Source-backed strengths
distinguishable from each other by acoustic pressure-response profiles
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distinguishable from each other by acoustic pressure-response profiles
Compared with hemisynthetic thiostrepton analogues
two new acoustic reporter genes and hemisynthetic thiostrepton analogues address a similar problem space.
Shared frame: same top-level item type
Compared with mMORp
two new acoustic reporter genes and mMORp address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Compared with split-ring metamaterial sensor with luxuriant gaps
two new acoustic reporter genes and split-ring metamaterial sensor with luxuriant gaps address a similar problem space.
Shared frame: same top-level item type
Ranked Citations
- 1.