Source 1primary paper2019Nature Communications
Toolkit/uSEE microscopy
uSEE microscopy
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The paper uses the upconversion-specific term uSEE microscopy when SEE microscopy is implemented with upconversion nanoparticles.
Usefulness & Problems
Why this is useful
uSEE is the upconversion-nanoparticle implementation of SEE microscopy.; Low-power 3D sub-diffraction imaging with upconversion nanoparticles
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uSEE is the upconversion-nanoparticle implementation of SEE microscopy.
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Low-power 3D sub-diffraction imaging with upconversion nanoparticles
Problem solved
It provides a named route for exploiting UCNP super-linearity for sub-diffraction imaging.; Applying SEE microscopy specifically to upconversion nanoparticle imaging
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It provides a named route for exploiting UCNP super-linearity for sub-diffraction imaging.
Source:
Applying SEE microscopy specifically to upconversion nanoparticle imaging
Problem links
Applying SEE microscopy specifically to upconversion nanoparticle imaging
LiteratureIt provides a named route for exploiting UCNP super-linearity for sub-diffraction imaging.
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It provides a named route for exploiting UCNP super-linearity for sub-diffraction imaging.
Published Workflows
Objective: Achieve 3D sub-diffraction imaging in a conventional confocal configuration by exploiting super-linear emitters.
Why it works: The method is reported to exploit super-linear emitters so that a conventional confocal configuration can produce sub-diffraction imaging.
super-linear excitation-emission behaviorupconversion emissionconfocal imaginguse of super-linear emitters
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
It requires SEE-compatible imaging plus upconversion nanoparticles as the emitting probe.; Requires upconversion nanoparticles
Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The paper implements uSEE microscopy using NaYF4:20%Yb,8%Tm upconversion nanoparticles excited at 976 nm with 455 nm emission for low-power 3D sub-diffraction imaging in a conventional confocal setup.
emission wavelength 455 nmexcitation wavelength 976 nm
The paper introduces super-linear excitation-emission microscopy as a method for 3D sub-diffraction imaging in a conventional confocal configuration.
The paper uses the term uSEE microscopy for the implementation of SEE microscopy with upconversion nanoparticles.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug usee-microscopy
The paper uses the upconversion-specific term uSEE microscopy when SEE microscopy is implemented with upconversion nanoparticles.
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application claimsupports
The paper implements uSEE microscopy using NaYF4:20%Yb,8%Tm upconversion nanoparticles excited at 976 nm with 455 nm emission for low-power 3D sub-diffraction imaging in a conventional confocal setup.
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method namingsupports
The paper uses the term uSEE microscopy for the implementation of SEE microscopy with upconversion nanoparticles.
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Comparisons
Source-stated alternatives
Related UCNP super-resolution methods mentioned in the web research summary include emission saturation nanoscopy and upconversion nonlinear structured illumination microscopy.
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Related UCNP super-resolution methods mentioned in the web research summary include emission saturation nanoscopy and upconversion nonlinear structured illumination microscopy.
Source-backed strengths
Uses upconversion nanoparticles in a conventional confocal setup; Reported as low-power imaging in the web research summary
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Uses upconversion nanoparticles in a conventional confocal setup
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Reported as low-power imaging in the web research summary
Compared with microscopy
Related UCNP super-resolution methods mentioned in the web research summary include emission saturation nanoscopy and upconversion nonlinear structured illumination microscopy.
Shared frame: source-stated alternative in extracted literature
Strengths here: Uses upconversion nanoparticles in a conventional confocal setup; Reported as low-power imaging in the web research summary.
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Related UCNP super-resolution methods mentioned in the web research summary include emission saturation nanoscopy and upconversion nonlinear structured illumination microscopy.
Compared with structured illumination
Related UCNP super-resolution methods mentioned in the web research summary include emission saturation nanoscopy and upconversion nonlinear structured illumination microscopy.
Shared frame: source-stated alternative in extracted literature
Strengths here: Uses upconversion nanoparticles in a conventional confocal setup; Reported as low-power imaging in the web research summary.
Source:
Related UCNP super-resolution methods mentioned in the web research summary include emission saturation nanoscopy and upconversion nonlinear structured illumination microscopy.
Compared with structured illumination microscopy
Related UCNP super-resolution methods mentioned in the web research summary include emission saturation nanoscopy and upconversion nonlinear structured illumination microscopy.
Shared frame: source-stated alternative in extracted literature
Strengths here: Uses upconversion nanoparticles in a conventional confocal setup; Reported as low-power imaging in the web research summary.
Source:
Related UCNP super-resolution methods mentioned in the web research summary include emission saturation nanoscopy and upconversion nonlinear structured illumination microscopy.
Compared with super-resolution microscopy
Related UCNP super-resolution methods mentioned in the web research summary include emission saturation nanoscopy and upconversion nonlinear structured illumination microscopy.
Shared frame: source-stated alternative in extracted literature
Strengths here: Uses upconversion nanoparticles in a conventional confocal setup; Reported as low-power imaging in the web research summary.
Source:
Related UCNP super-resolution methods mentioned in the web research summary include emission saturation nanoscopy and upconversion nonlinear structured illumination microscopy.
Ranked Citations
- 1.