Source 1review2024Journal of Cell Science
Toolkit/volume electron microscopy
volume electron microscopy
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
In this Review, we explore new insights from studies using super-resolution and volume electron microscopy into the nanoscale organization of these junctional complexes...
Usefulness & Problems
Why this is useful
Volume electron microscopy is described as a source of new insights into nanoscale organization of cell-cell junctions. The review also links it to understanding junctions within their surrounding membrane and topographic context.; studying nanoscale organization of junctional complexes; integrating junctional architecture with membrane morphology and cellular topography
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Volume electron microscopy is described as a source of new insights into nanoscale organization of cell-cell junctions. The review also links it to understanding junctions within their surrounding membrane and topographic context.
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studying nanoscale organization of junctional complexes
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integrating junctional architecture with membrane morphology and cellular topography
Problem solved
It helps place nanoscale junctional architectures into the membrane morphology and cellular topography in which they are embedded. This addresses context that isolated molecular views may miss.; provides structural context for junctional architectures within surrounding cellular topology
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It helps place nanoscale junctional architectures into the membrane morphology and cellular topography in which they are embedded. This addresses context that isolated molecular views may miss.
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provides structural context for junctional architectures within surrounding cellular topology
Problem links
provides structural context for junctional architectures within surrounding cellular topology
LiteratureIt helps place nanoscale junctional architectures into the membrane morphology and cellular topography in which they are embedded. This addresses context that isolated molecular views may miss.
Source:
It helps place nanoscale junctional architectures into the membrane morphology and cellular topography in which they are embedded. This addresses context that isolated molecular views may miss.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
It requires electron microscopy-based imaging workflows capable of volumetric structural analysis. The abstract does not provide protocol details.; requires electron microscopy workflows suitable for volumetric structural imaging
The abstract does not state that volume electron microscopy alone reports junctional dynamics in live settings. Dynamic information is instead discussed more broadly alongside live fluorescence microscopy and other tools.; the abstract does not specify exact volume EM implementations or throughput
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Electron microscopy and live fluorescence microscopy have significantly enhanced understanding of molecular mechanisms regulating junctional dynamics during homeostasis, development, and disease.
Junctional architectures should be integrated with membrane morphology and cellular topography in which the junctions are embedded.
Junction-related biosensors, cytoskeletal-related biosensors, and optogenetic probes have contributed to advances in understanding junctional dynamics across cellular environments.
Studying nanoscale architectures of tight junctions, adherens junctions, and desmosomes is crucial for understanding the complexity of cell-cell adhesions.
Super-resolution microscopy and volume electron microscopy have provided new insights into the nanoscale organization of cell-cell junctional complexes and their relationships to the junction-associated cytoskeleton, neighboring organelles, and the plasma membrane.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug volume-electron-microscopy
In this Review, we explore new insights from studies using super-resolution and volume electron microscopy into the nanoscale organization of these junctional complexes...
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review summarysupports
Junctional architectures should be integrated with membrane morphology and cellular topography in which the junctions are embedded.
Source:
review summarysupports
Studying nanoscale architectures of tight junctions, adherens junctions, and desmosomes is crucial for understanding the complexity of cell-cell adhesions.
Source:
review summarysupports
Super-resolution microscopy and volume electron microscopy have provided new insights into the nanoscale organization of cell-cell junctional complexes and their relationships to the junction-associated cytoskeleton, neighboring organelles, and the plasma membrane.
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Comparisons
Source-stated alternatives
The abstract mentions electron microscopy broadly, live fluorescence microscopy, and super-resolution microscopy as related approaches.
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The abstract mentions electron microscopy broadly, live fluorescence microscopy, and super-resolution microscopy as related approaches.
Source-backed strengths
supports integration of junctional architecture with membrane morphology and cellular topography
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supports integration of junctional architecture with membrane morphology and cellular topography
Compared with electron microscopy
The abstract mentions electron microscopy broadly, live fluorescence microscopy, and super-resolution microscopy as related approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports integration of junctional architecture with membrane morphology and cellular topography.
Relative tradeoffs: the abstract does not specify exact volume EM implementations or throughput.
Source:
The abstract mentions electron microscopy broadly, live fluorescence microscopy, and super-resolution microscopy as related approaches.
Compared with fluorescence microscopy
The abstract mentions electron microscopy broadly, live fluorescence microscopy, and super-resolution microscopy as related approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports integration of junctional architecture with membrane morphology and cellular topography.
Relative tradeoffs: the abstract does not specify exact volume EM implementations or throughput.
Source:
The abstract mentions electron microscopy broadly, live fluorescence microscopy, and super-resolution microscopy as related approaches.
Compared with live fluorescence microscopy
The abstract mentions electron microscopy broadly, live fluorescence microscopy, and super-resolution microscopy as related approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports integration of junctional architecture with membrane morphology and cellular topography.
Relative tradeoffs: the abstract does not specify exact volume EM implementations or throughput.
Source:
The abstract mentions electron microscopy broadly, live fluorescence microscopy, and super-resolution microscopy as related approaches.
Compared with microscopy
The abstract mentions electron microscopy broadly, live fluorescence microscopy, and super-resolution microscopy as related approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports integration of junctional architecture with membrane morphology and cellular topography.
Relative tradeoffs: the abstract does not specify exact volume EM implementations or throughput.
Source:
The abstract mentions electron microscopy broadly, live fluorescence microscopy, and super-resolution microscopy as related approaches.
Compared with super-resolution microscopy
The abstract mentions electron microscopy broadly, live fluorescence microscopy, and super-resolution microscopy as related approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports integration of junctional architecture with membrane morphology and cellular topography.
Relative tradeoffs: the abstract does not specify exact volume EM implementations or throughput.
Source:
The abstract mentions electron microscopy broadly, live fluorescence microscopy, and super-resolution microscopy as related approaches.
Ranked Citations
- 1.