Source 1primary paper2025MED
Toolkit/YwbIR-based whole-cell bacterial acetic acid biosensor
YwbIR-based whole-cell bacterial acetic acid biosensor
Also known as: transcription-based whole-cell biosensor, whole-cell bacterial biosensor
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Here, we present an alternative tool for AC detection using a whole-cell bacterial biosensor, which utilises the YwbIR transcriptional regulator from Bacillus subtilis.
Usefulness & Problems
Why this is useful
This tool is a transcription-based whole-cell bacterial biosensor for detecting acetic acid. It uses the Bacillus subtilis YwbIR transcriptional regulator to produce an acetic-acid-responsive readout.; acetic acid detection in fermentation processes; real-time monitoring of acetic acid buildup; headspace detection in ethanol-rich fermentation matrices
Source:
This tool is a transcription-based whole-cell bacterial biosensor for detecting acetic acid. It uses the Bacillus subtilis YwbIR transcriptional regulator to produce an acetic-acid-responsive readout.
Source:
acetic acid detection in fermentation processes
Source:
real-time monitoring of acetic acid buildup
Source:
headspace detection in ethanol-rich fermentation matrices
Problem solved
It addresses the need for lower-cost, real-time monitoring of acetic acid accumulation during fermentation, especially for early spoilage detection. The paper positions it as an alternative to slower and more expensive analytical chemistry methods.; provides a lower-cost alternative to conventional acetic acid detection methods; supports earlier identification of spoilage-associated acetic acid accumulation
Source:
It addresses the need for lower-cost, real-time monitoring of acetic acid accumulation during fermentation, especially for early spoilage detection. The paper positions it as an alternative to slower and more expensive analytical chemistry methods.
Source:
provides a lower-cost alternative to conventional acetic acid detection methods
Source:
supports earlier identification of spoilage-associated acetic acid accumulation
Problem links
provides a lower-cost alternative to conventional acetic acid detection methods
LiteratureIt addresses the need for lower-cost, real-time monitoring of acetic acid accumulation during fermentation, especially for early spoilage detection. The paper positions it as an alternative to slower and more expensive analytical chemistry methods.
Source:
It addresses the need for lower-cost, real-time monitoring of acetic acid accumulation during fermentation, especially for early spoilage detection. The paper positions it as an alternative to slower and more expensive analytical chemistry methods.
supports earlier identification of spoilage-associated acetic acid accumulation
LiteratureIt addresses the need for lower-cost, real-time monitoring of acetic acid accumulation during fermentation, especially for early spoilage detection. The paper positions it as an alternative to slower and more expensive analytical chemistry methods.
Source:
It addresses the need for lower-cost, real-time monitoring of acetic acid accumulation during fermentation, especially for early spoilage detection. The paper positions it as an alternative to slower and more expensive analytical chemistry methods.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
Functional AssayTarget processes
transcriptionImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The biosensor requires a bacterial whole-cell chassis and the YwbIR regulatory module from Bacillus subtilis. The abstract also indicates use in ethanol-rich matrices and headspace detection settings.; uses the YwbIR transcriptional regulator from Bacillus subtilis; implemented as a whole-cell bacterial biosensor
Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The biosensor offers a low-cost solution for real-time acetic acid monitoring to support timely intervention before spoilage in fermentation-driven food and beverage production.
The biosensor enables headspace detection of acetic acid.
The biosensor retains functionality in ethanol-rich matrices up to 14.5% v/v ethanol.
ethanol compatibility limit 14.5 % v/v
The biosensor shows 5-8 fold induction at wine spoilage-relevant acetic acid concentrations.
induction fold change 5-8 fold
The YwbIR-based whole-cell bacterial acetic acid biosensor shows a linear response from 0 to 1.0 g/L with R^2 = 0.97.
linear response range 0 to 1.0 g/LR^2 0.97
Specificity assays and molecular docking analyses indicate that the biosensor has high affinity for acetic acid over other volatile fatty acids.
A whole-cell bacterial biosensor using the Bacillus subtilis YwbIR transcriptional regulator can detect acetic acid.
Approval Evidence
1 source7 linked approval claimsfirst-pass slug ywbir-based-whole-cell-bacterial-acetic-acid-biosensor
Here, we present an alternative tool for AC detection using a whole-cell bacterial biosensor, which utilises the YwbIR transcriptional regulator from Bacillus subtilis.
Source:
application valuesupports
The biosensor offers a low-cost solution for real-time acetic acid monitoring to support timely intervention before spoilage in fermentation-driven food and beverage production.
Source:
capabilitysupports
The biosensor enables headspace detection of acetic acid.
Source:
matrix compatibilitysupports
The biosensor retains functionality in ethanol-rich matrices up to 14.5% v/v ethanol.
Source:
performancesupports
The biosensor shows 5-8 fold induction at wine spoilage-relevant acetic acid concentrations.
Source:
performancesupports
The YwbIR-based whole-cell bacterial acetic acid biosensor shows a linear response from 0 to 1.0 g/L with R^2 = 0.97.
Source:
specificitysupports
Specificity assays and molecular docking analyses indicate that the biosensor has high affinity for acetic acid over other volatile fatty acids.
Source:
tool functionsupports
A whole-cell bacterial biosensor using the Bacillus subtilis YwbIR transcriptional regulator can detect acetic acid.
Source:
Comparisons
Source-stated alternatives
The abstract contrasts this biosensor with steam distillation, GC-MS, and HPLC as conventional acetic acid detection methods.
Source:
The abstract contrasts this biosensor with steam distillation, GC-MS, and HPLC as conventional acetic acid detection methods.
Source-backed strengths
linear response from 0 to 1.0 g/L; 5-8 fold induction at wine spoilage-relevant concentrations; retains functionality up to 14.5% v/v ethanol; enables headspace detection; shows high affinity for acetic acid over other volatile fatty acids
Source:
linear response from 0 to 1.0 g/L
Source:
5-8 fold induction at wine spoilage-relevant concentrations
Source:
retains functionality up to 14.5% v/v ethanol
Source:
enables headspace detection
Source:
shows high affinity for acetic acid over other volatile fatty acids
Compared with 4pLRE-cPAOX1
YwbIR-based whole-cell bacterial acetic acid biosensor and 4pLRE-cPAOX1 address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription
Strengths here: looks easier to implement in practice.
Compared with blue-light-activated DNA template ON switch
YwbIR-based whole-cell bacterial acetic acid biosensor and blue-light-activated DNA template ON switch address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription
Strengths here: looks easier to implement in practice.
Compared with receptor-based sensors
YwbIR-based whole-cell bacterial acetic acid biosensor and receptor-based sensors address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription
Ranked Citations
- 1.