Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
5 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-5 of 5
Fus1 is a formin protein domain context in which an intrinsically disordered region is reported to drive condensation of the fusion focus. This condensation activity is essential for cell-cell fusion.
Rationally designed PopZ mutants are engineered variants of the bacterial condensate-forming protein PopZ in which sequence changes in a disordered domain are used to alter condensate behavior. Reported evidence indicates that these variants tune condensate function and that PopZ can be repurposed as a modular platform for synthetic condensates in human cells.
Synthetic PopZ condensates in human cells are an engineered repurposing of the bacterial PopZ condensate system as a modular platform for forming biomolecular condensates in human cells. The reported design principle is that sequence variation in a disordered domain can tune condensate function.
Light-triggered liquid-liquid phase separation modules are optogenetic multi-component switches that use light to induce biomolecular condensation. They are described as co-localizing and condensing key enzymes to enhance catalytic efficiency.
Among these, the intracellular systems (i.e., optoDroplet, Corelet, PixELL, CasDrop, and other optogenetic systems) that enable the photo-mediated control over biomolecular condensation are highlighted.