Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
SPI1085g3 Cys448Ser variant is a cyanobacteriochrome GAF-domain mutant derived from the Spirulina protein SPI1085g3, generated by replacing Cys448 with Ser. In the reported study, this variant retained the photoswitchable fluorescent behavior of SPI1085g3 while showing slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion.
SPI1085g3 is a red/green cyanobacteriochrome GAF domain from Spirulina subsalsa. It photoconverts from a red-absorbing dark Pr state to an orange-absorbing Po state, and the Pr state exhibits intense red fluorescence that is lost upon photoconversion and recovers through moderate dark reversion.