PALM
Assay MethodThe anchor paper explicitly reports super-resolution imaging under a PALM setup.
CFBacMamMusHumTxRep
Ev 37Rep 20Pr 83
Toolkit Items
Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
5 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
▾Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 1
Mechanisms
Layer 2
Architectures
▾Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 2
Architectures
Layer 3
Components
▾Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Layer 3
Components
Technique Branch
Layer 1
Approaches
▾High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 1
Approaches
Layer 2
Methods
▾Concrete methods used to design, build, verify, or characterize engineered systems.
Layer 2
Methods
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photoconversion
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SPI1085g3 Cys448Ser variant
Protein DomainSPI1085g3 Cys448Ser variant is a cyanobacteriochrome GAF-domain mutant derived from the Spirulina protein SPI1085g3, generated by replacing Cys448 with Ser. In the reported study, this variant retained the photoswitchable fluorescent behavior of SPI1085g3 while showing slightly improved fluorescence quantum yield and nearly 13-fold faster dark reversion.
CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71
Dendra2 fused to actin
Construct PatternSince 2014blinkingphotobleachingphotoconversionredox-based bleaching and blinkingthermal reactivation
Here, we measured phototransformation quantum yields for Dendra2 fused to actin in fixed mammalian cells in typical (F)-PALM experiments.
CFBacMamMusHumTxRep
Ev 32Rep 9Pr 59
optogenetic multiplexing method
Engineering MethodSince 2017photoconversion
This optogenetic multiplexing method is an engineering approach for programming two simultaneous and independent gene expression signals within the same cell using light. The reported method uses a photoconversion model to compensate for spectral cross-reactivity between two optogenetic sensors.
CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59
SPI1085g3
Protein DomainSPI1085g3 is a red/green cyanobacteriochrome GAF domain from Spirulina subsalsa. It photoconverts from a red-absorbing dark Pr state to an orange-absorbing Po state, and the Pr state exhibits intense red fluorescence that is lost upon photoconversion and recovers through moderate dark reversion.
CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59
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