Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
8 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-8 of 8
Humanized mNeonGreen is a human codon-optimized cDNA construct encoding the fluorescent protein mNeonGreen for mammalian expression. In HEK293 cells, it produced higher fluorescent intensity than the original mNeonGreen, and derivative constructs supported anti-FLAG detection and mitochondrial targeting.
FMN fluorescence is an optical metabolic imaging assay based on flavin mononucleotide autofluorescence. The supplied evidence indicates potential use for visualization and classification of brain tumor tissue during surgery.
miRFP720 is a monomeric near-infrared fluorescent protein reported in the cited study as the most red-shifted monomeric NIR fluorescent protein. It functions as a fluorescent component for reporter construction that can be imaged with reduced spectral interference from visible-range probes and blue-green optogenetic tools.
a near-infrared rhodamine dye, prepared by photoirradiation and used as a fluorescent probe in cell imaging
Photoreceptor-derived biosensors are engineered protein tools built from natural light-sensing photoreceptors as molecular templates. The cited review presents natural photoreceptors as a broad source for developing biosensors, fluorescent proteins, and optogenetic tools responsive to light.
Considering their preferential interaction with biliverdin IXα as endogenous cofactor, they have recently been used for creating optogenetic tools and engineering fluorescent probes.
The paper introduces miRFP670nano3, a 17 kDa single-domain cyanobacteriochrome-derived near-infrared fluorescent protein.
Among them is the bimodal photoswitchable Neorhodopsin (NeoR), which exhibits a near-infrared absorbing, highly fluorescent state.