Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-3 of 3
YtvA from Bacillus subtilis is a LOV-domain photoswitch reported in this evidence set as a homodimeric light-responsive protein domain. It was highlighted in an optogenetics context because its relaxation half-life is longer than that of Avena sativa LOV2, suggesting potential utility as a light-controlled module.
The light-oxygen-voltage sensing (LOV) domain is a blue light-responsive protein domain used in optogenetic constructs to confer light-dependent control over coupled signaling effectors. The supplied evidence specifically describes it as a blue light homodimerizing LOV domain.
The Rhodobacter sphaeroides light-oxygen-voltage domain (RsLOV) is a homodimeric LOV photosensory protein domain from Rhodobacter sphaeroides. Reported engineering results indicate that fusion of RsLOV to Cas9-derived effector variants can confer light sensitivity, and the same domain also imparted strong temperature sensitivity in that study.