Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

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Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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light-induced generation of reactive intermediates from aryl azides

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red-light-induced extracellular protein labeling

Engineering Method
Since 2023electron-transferenergy-transferlight-induced generation of reactive intermediates from aryl azidesphotolysis

Red-light-induced extracellular protein labeling is a visible-light aryl azide labeling approach for live-cell protein modification at the cell exterior. In this method, light activation of aryl azides generates highly reactive intermediates that enable extracellular protein labeling, and the cited review identifies recent advances specifically under red light.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 59
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