Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

4 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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photolysis

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aryl azides

Construct Pattern
Since 2023electron-transfer activationenergy-transfer activationlight-triggered generation of reactive intermediatesphotolysis

Aryl azides are light-activatable chemical groups that remain chemically inert under physiological conditions until irradiation induces reactive intermediates. In visible-light-enabled live-cell settings, they have been used for protein labeling applications including functionalization, crosslinking, and profiling in extracellular and intracellular contexts.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 59

red-light-induced extracellular protein labeling

Engineering Method
Since 2023electron-transferenergy-transferlight-induced generation of reactive intermediates from aryl azidesphotolysis

Red-light-induced extracellular protein labeling is a visible-light aryl azide labeling approach for live-cell protein modification at the cell exterior. In this method, light activation of aryl azides generates highly reactive intermediates that enable extracellular protein labeling, and the cited review identifies recent advances specifically under red light.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 59

visible-light-induced protein labeling

Engineering Method
Since 2023crosslinkingelectron transferelectron-transferenergy transferenergy-transferphotolysisreactive-intermediate generation from aryl azides

Visible-light-induced protein labeling is a live-cell chemical biology method that uses visible-light irradiation to activate aryl azides and generate reactive intermediates for protein labeling. Reported applications include protein functionalization, crosslinking, and profiling in live cells, with both extracellular and intracellular implementations.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 59

visible-light-induced protein labeling with aryl azides

Engineering Method
Since 2023electron-transfer activationenergy-transfer activationlight-triggered generation of reactive intermediatesphotolysisprotein crosslinking

Visible-light-induced protein labeling with aryl azides is an engineering method for live-cell protein labeling in which aryl azides are activated by visible light to generate reactive intermediates. Reported applications include protein functionalization, crosslinking, and profiling in extracellular and intracellular live-cell contexts, with recent advances in red-light-induced extracellular labeling.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 59
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