Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
6 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-6 of 6
The engineered optogenetic on switch is a light-inducible multi-component system that converts a transient blue light input into permanent transgene expression. It has been applied to drive recombination-linked gene expression programs, including spatially patterned differentiation in culture and pro-angiogenic factor expression in vivo.
Multiplexed engineering refers here to the use of the CRISPR/Cas toolbox for simultaneous genome engineering tasks in microbial biotechnology. The cited review places this approach in the context of metabolic engineering and high-throughput screening for production of chemicals and natural compounds.
Comprehensive insertion libraries are a high-throughput engineering method in which many insertion variants are generated and screened. In the cited context, they are discussed as an approach that could accelerate creation of stimulus-responsive receptor–protein chimeras.
we developed a dCas9-miniRecTE editor that enhances large-fragment integration without introducing double-strand breaks in human cells and primary mouse neurons, achieving ∼20% kilobase-scale knock-in efficiency.
The anchor paper is a zebrafish neurogenetics resource paper centered on BAC recombineering, Tol2-mediated BAC transgenesis, whole-brain image registration, and intersectional Gal4/Cre targeting.
we also summarize recent developments in zebrafish genetics and small molecule screening, which markedly enhance the disease modelling and the discovery of novel drug targets.