Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

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Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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Cry2-METTL3 fusion construct

Construct Pattern

The Cry2-METTL3 fusion construct is an optogenetic domain-fusion reporter in which METTL3 is fused to Cry2 to track components of the mRNA m6A methyltransferase complex. In the cited study, it was used to monitor assembly behavior associated with METTL3 phase separation.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71
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