Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
12 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-12 of 12
Many draw inspiration from widely successful fluorescence-based techniques such as stimulated emission depletion (STED) microscopy, photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM).
The anchor paper explicitly reports super-resolution imaging under a PALM setup.
Many draw inspiration from widely successful fluorescence-based techniques such as stimulated emission depletion (STED) microscopy, photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM).
ImageJ is a versatile and powerful tool for quantitative image analysis in microscopy.
The supplied source summary states that the review explicitly covers SMLM, including STORM/dSTORM.
we have super-resolution microscopy techniques such as STED, PALM and STORM able to achieve 10 to 20 nm spatial resolution in single molecule localisation
The supplied source summary states that the review explicitly covers SMLM, including STORM/dSTORM.
The supplied source summary states that the review explicitly covers SMLM, including GSDIM.
We outline the technical developments that have led to the emergence of localization microscopy techniques from single-molecule spectroscopy.
Today, single molecule super-resolution imaging is routinely used in the study of macromolecular function and structure in the cell.
All localization precision and patterned illumination techniques-such as photo-activation localization microscopy ... take advantage of these inherent switching properties to achieve superior spatial resolution.
Progress in labeling, optics, and single-molecule localization algorithms is being rapid, and it is likely to provide real insight into DNA structuring in cells and materials.