dCas9*_PhlF is a bacterial CRISPR-based transcriptional switch comprising a non-toxic dCas9* variant with the R1335K PAM-binding mutation fused to the PhlF repressor. The fusion recovered DNA-binding-dependent repression and enabled sgRNA-programmed NOT gate behavior that depends on both an sgRNA target site and a PhlF operator.
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Mechanism Concept
RNA Binding
A protein or RNA element binds RNA to control translation or stability.
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Architectures
1 of 1Components
1 of 1The web research summary states that the review describes PAL as a bacterial LOV receptor that interacts with RNA and supports light-regulated translation via aptamers.