Source 1primary paper2016Methods in molecular biology
Toolkit/all-optical framework for functional testing of opsin responsiveness in cFB
all-optical framework for functional testing of opsin responsiveness in cFB
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The all-optical framework for functional testing of opsin responsiveness in cardiac fibroblasts is an assay method described to evaluate whether virally introduced optogenetic actuators are functionally responsive in primary cFB. In the cited study, it provides an optical functional readout for opsin-expressing cardiac fibroblasts and is associated with co-culture conditions that can yield a light-sensitive excitable cardiac syncytium.
Usefulness & Problems
Why this is useful
This framework is useful for functionally verifying that delivered opsins in cardiac fibroblasts respond to light, rather than relying only on expression or delivery metrics. It supports evaluation of optogenetically modified cFB in experimental settings involving non-transformed cardiomyocyte co-culture.
Problem solved
It addresses the specific need to test whether optogenetic tools introduced into primary cardiac fibroblasts are functionally responsive. The available evidence does not indicate that the framework by itself resolves broader questions such as in vivo performance or comprehensive electrical coupling analysis.
Problem links
provides a way to test whether delivered opsins are functionally responsive in cardiac fibroblasts
LiteratureIt addresses the need to functionally verify that delivered optogenetic tools are responsive in the target cells.
Source:
It addresses the need to functionally verify that delivered optogenetic tools are responsive in the target cells.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
optical activation of opsinsoptical activation of opsinsoptical activation of opsinsoptical functional readoutoptical functional readoutoptical functional readoutTechniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The method requires opsin-expressing cardiac fibroblasts and an all-optical experimental setup, although the abstract-level evidence does not detail instrumentation or construct design. The cited application context includes viral delivery of optogenetic tools in primary cFB and co-culture with non-transformed cardiomyocytes.
The supplied evidence does not specify the optical hardware, stimulation wavelengths, reporter modality, quantitative performance, or which opsins were tested within this framework. Independent replication is not provided in the evidence, and validation appears limited to the reported study context.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
Specific co-culture conditions of optogenetically modified cardiac fibroblasts with non-transformed cardiomyocytes can be used to obtain a light-sensitive excitable cardiac syncytium.
An all-optical framework is described for functional testing of opsin responsiveness in cardiac fibroblasts.
An all-optical framework is described for functional testing of opsin responsiveness in cardiac fibroblasts.
An all-optical framework is described for functional testing of opsin responsiveness in cardiac fibroblasts.
An all-optical framework is described for functional testing of opsin responsiveness in cardiac fibroblasts.
An all-optical framework is described for functional testing of opsin responsiveness in cardiac fibroblasts.
An all-optical framework is described for functional testing of opsin responsiveness in cardiac fibroblasts.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adenoviral infection can introduce ChR2(H134R) and ArchT into primary cardiac fibroblasts in vitro and yield quick, robust, and consistent expression.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Adjusting multiplicity of infection and virus incubation duration is presented as a way to generalize the adenoviral infection method across different lab settings or cell types.
Comparisons
Source-backed strengths
A key strength is that the method is explicitly all-optical, enabling functional testing of opsin responsiveness in cFB without a non-optical readout being described in the provided evidence. The associated study also reports that specific co-culture conditions with non-transformed cardiomyocytes can produce a light-sensitive excitable cardiac syncytium.
Compared with Field-domain rapid-scan EPR at 240 GHz
all-optical framework for functional testing of opsin responsiveness in cFB and Field-domain rapid-scan EPR at 240 GHz address a similar problem space.
Shared frame: same top-level item type
Compared with fluorescence line narrowing
all-optical framework for functional testing of opsin responsiveness in cFB and fluorescence line narrowing address a similar problem space.
Shared frame: same top-level item type
Compared with native green gel system
all-optical framework for functional testing of opsin responsiveness in cFB and native green gel system address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.