Source 1review2001Biochemistry (Moscow)
Toolkit/sub-picosecond pump-probe analysis of bacteriorhodopsin pigments
sub-picosecond pump-probe analysis of bacteriorhodopsin pigments
Also known as: pump-probe experiments with sub-picosecond time resolution
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The early photophysical events are analyzed in view of recent pump-probe experiments with sub-picosecond time resolution comparing the behavior of bR pigments with those of model protonated Schiff bases in solution.
Usefulness & Problems
Why this is useful
This assay measures ultrafast photophysical behavior of bacteriorhodopsin pigments after excitation. In the review, it is used to compare artificial pigments with model protonated Schiff bases in solution.; measuring early photophysical events in bacteriorhodopsin pigments; comparing artificial bacteriorhodopsin pigments to model protonated Schiff bases in solution
Source:
This assay measures ultrafast photophysical behavior of bacteriorhodopsin pigments after excitation. In the review, it is used to compare artificial pigments with model protonated Schiff bases in solution.
Source:
measuring early photophysical events in bacteriorhodopsin pigments
Source:
comparing artificial bacteriorhodopsin pigments to model protonated Schiff bases in solution
Problem solved
It resolves the earliest relaxation events that are too fast for slower spectroscopic methods.; provides time-resolved access to ultrafast excited-state relaxation behavior
Source:
It resolves the earliest relaxation events that are too fast for slower spectroscopic methods.
Source:
provides time-resolved access to ultrafast excited-state relaxation behavior
Problem links
provides time-resolved access to ultrafast excited-state relaxation behavior
LiteratureIt resolves the earliest relaxation events that are too fast for slower spectroscopic methods.
Source:
It resolves the earliest relaxation events that are too fast for slower spectroscopic methods.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
excited-state relaxation from the franck-condon stateprotein-matrix-catalyzed relaxationultrafast photoexcitation and pump-probe transient analysisTechniques
Functional AssayTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
It requires sub-picosecond pump-probe spectroscopy and suitable pigment or model-compound samples.; requires ultrafast pump-probe instrumentation and comparison samples such as model protonated Schiff bases in solution
Needs compatible illumination hardware and optical access. Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
In bacteriorhodopsin, initial relaxation out of the Franck-Condon state does not involve substantial C13=C14 torsional motion and is considerably catalyzed by the protein matrix.
It is concluded that in bR the initial relaxation out of the Franck-Condon (FC) state does not involve substantial C13=C14 torsional motion and is considerably catalyzed by the protein matrix.
Protein activation in bacteriorhodopsin can begin before decay of the relaxed fluorescent state through a mechanism that does not require double-bond isomerization.
Prior to the decay of the relaxed fluorescent state (FS or I state), the protein is activated via a mechanism that does not require double bond isomerization.
Approval Evidence
1 source1 linked approval claimfirst-pass slug sub-picosecond-pump-probe-analysis-of-bacteriorhodopsin-pigments
The early photophysical events are analyzed in view of recent pump-probe experiments with sub-picosecond time resolution comparing the behavior of bR pigments with those of model protonated Schiff bases in solution.
Source:
mechanistic summarysupports
In bacteriorhodopsin, initial relaxation out of the Franck-Condon state does not involve substantial C13=C14 torsional motion and is considerably catalyzed by the protein matrix.
It is concluded that in bR the initial relaxation out of the Franck-Condon (FC) state does not involve substantial C13=C14 torsional motion and is considerably catalyzed by the protein matrix.
Source:
Comparisons
Source-stated alternatives
The abstract also mentions hydroxylamine-based Schiff-base cleavage and spin-label EPR as complementary approaches.
Source:
The abstract also mentions hydroxylamine-based Schiff-base cleavage and spin-label EPR as complementary approaches.
Source-backed strengths
sub-picosecond time resolution is suitable for primary light-induced events
Source:
sub-picosecond time resolution is suitable for primary light-induced events
Compared with CLARITY technology
sub-picosecond pump-probe analysis of bacteriorhodopsin pigments and CLARITY technology address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with Langendorff perfused heart electrical recordings
sub-picosecond pump-probe analysis of bacteriorhodopsin pigments and Langendorff perfused heart electrical recordings address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with native green gel system
sub-picosecond pump-probe analysis of bacteriorhodopsin pigments and native green gel system address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.