Toolkit/antisense oligodeoxynucleotide against Per1

antisense oligodeoxynucleotide against Per1

Construct Pattern·Research·Since 2003

Also known as: antisense ODN against Per1

Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

The antisense oligodeoxynucleotide against Per1 is a nucleic acid perturbation reagent used in the rat suprachiasmatic nucleus to suppress Per1 function during circadian signaling experiments. In the cited study, this antisense ODN blocked glutamate-induced phase advances, implicating Per1 in light-responsive clock resetting.

Usefulness & Problems

Why this is useful

This reagent is useful for testing whether Per1 is required in the signaling pathway that links light-stimulated glutamatergic input to circadian phase shifting in the suprachiasmatic nucleus. The available evidence supports its use as a loss-of-function perturbation in rat SCN tissue.

Problem solved

It addresses the problem of causally probing Per1 function in glutamate-triggered circadian phase advance responses. Specifically, it enables experimental suppression of Per1 to test whether this gene is necessary for SCN clock resetting downstream of light-associated glutamatergic neurotransmission.

Problem links

Need conditional recombination or state switching

Derived

The antisense oligodeoxynucleotide against Per1 is a nucleic acid perturbation reagent used in the rat suprachiasmatic nucleus to suppress Per1 function during circadian signaling experiments. In the cited study, this antisense ODN blocked glutamate-induced phase advances, implicating Per1 in light-responsive clock resetting.

Need precise spatiotemporal control with light input

Derived

The antisense oligodeoxynucleotide against Per1 is a nucleic acid perturbation reagent used in the rat suprachiasmatic nucleus to suppress Per1 function during circadian signaling experiments. In the cited study, this antisense ODN blocked glutamate-induced phase advances, implicating Per1 in light-responsive clock resetting.

Taxonomy & Function

Primary hierarchy

Mechanism Branch

Architecture: A reusable architecture pattern for arranging parts into an engineered system.

Techniques

No technique tags yet.

Target processes

recombination

Input: Light

Implementation Constraints

cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulator

The available evidence indicates use in the rat suprachiasmatic nucleus in the context of glutamate-induced phase-advance assays. No sequence, chemistry, dosing, delivery method, or expression-system details are provided in the supplied evidence.

The evidence is limited to a single cited study in rat suprachiasmatic nucleus and reports a phase-shift phenotype without detailed construct, sequence, or delivery information here. The supplied evidence does not document independent replication, quantitative knockdown efficiency, off-target assessment, or validation in other organisms or preparations.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Observations

successMouseapplication demoratsuprachiasmatic nucleus

Inferred from claim c2 during normalization. Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus. Derived from claim c2. Section: abstract. Quoted text: Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1

Source:

successMouseapplication demoratsuprachiasmatic nucleus

Inferred from claim c2 during normalization. Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus. Derived from claim c2. Section: abstract. Quoted text: Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1

Source:

successMouseapplication demoratsuprachiasmatic nucleus

Inferred from claim c2 during normalization. Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus. Derived from claim c2. Section: abstract. Quoted text: Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1

Source:

successMouseapplication demoratsuprachiasmatic nucleus

Inferred from claim c2 during normalization. Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus. Derived from claim c2. Section: abstract. Quoted text: Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1

Source:

successMouseapplication demoratsuprachiasmatic nucleus

Inferred from claim c2 during normalization. Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus. Derived from claim c2. Section: abstract. Quoted text: Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1

Source:

successMouseapplication demoratsuprachiasmatic nucleus

Inferred from claim c2 during normalization. Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus. Derived from claim c2. Section: abstract. Quoted text: Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1

Source:

successMouseapplication demoratsuprachiasmatic nucleus

Inferred from claim c2 during normalization. Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus. Derived from claim c2. Section: abstract. Quoted text: Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1

Source:

Supporting Sources

Ranked Claims

Claim 1gene expression effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced accumulation of Per1 mRNA.

Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA.
Section: abstract
Claim 2gene expression effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced accumulation of Per1 mRNA.

Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA.
Section: abstract
Claim 3gene expression effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced accumulation of Per1 mRNA.

Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA.
Section: abstract
Claim 4gene expression effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced accumulation of Per1 mRNA.

Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA.
Section: abstract
Claim 5gene expression effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced accumulation of Per1 mRNA.

Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA.
Section: abstract
Claim 6gene expression effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced accumulation of Per1 mRNA.

Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA.
Section: abstract
Claim 7gene expression effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced accumulation of Per1 mRNA.

Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA.
Section: abstract
Claim 8pathway component rolesupports2003Source 1needs review

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.
Section: abstract
Claim 9pathway component rolesupports2003Source 1needs review

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.
Section: abstract
Claim 10pathway component rolesupports2003Source 1needs review

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.
Section: abstract
Claim 11pathway component rolesupports2003Source 1needs review

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.
Section: abstract
Claim 12pathway component rolesupports2003Source 1needs review

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.
Section: abstract
Claim 13pathway component rolesupports2003Source 1needs review

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.
Section: abstract
Claim 14pathway component rolesupports2003Source 1needs review

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.
Section: abstract
Claim 15perturbation effectsupports2003Source 1needs review

Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1
Section: abstract
Claim 16perturbation effectsupports2003Source 1needs review

Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1
Section: abstract
Claim 17perturbation effectsupports2003Source 1needs review

Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1
Section: abstract
Claim 18perturbation effectsupports2003Source 1needs review

Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1
Section: abstract
Claim 19perturbation effectsupports2003Source 1needs review

Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1
Section: abstract
Claim 20perturbation effectsupports2003Source 1needs review

Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1
Section: abstract
Claim 21perturbation effectsupports2003Source 1needs review

Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1
Section: abstract
Claim 22perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.
Section: abstract
Claim 23perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.
Section: abstract
Claim 24perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.
Section: abstract
Claim 25perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.
Section: abstract
Claim 26perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.
Section: abstract
Claim 27perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.
Section: abstract
Claim 28perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.
Section: abstract
Claim 29perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks light-induced phase advances in vivo.

CRE-decoy ODN also blocked light-induced phase advances in vivo.
Section: abstract
Claim 30perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks light-induced phase advances in vivo.

CRE-decoy ODN also blocked light-induced phase advances in vivo.
Section: abstract
Claim 31perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks light-induced phase advances in vivo.

CRE-decoy ODN also blocked light-induced phase advances in vivo.
Section: abstract
Claim 32perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks light-induced phase advances in vivo.

CRE-decoy ODN also blocked light-induced phase advances in vivo.
Section: abstract
Claim 33perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks light-induced phase advances in vivo.

CRE-decoy ODN also blocked light-induced phase advances in vivo.
Section: abstract
Claim 34perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks light-induced phase advances in vivo.

CRE-decoy ODN also blocked light-induced phase advances in vivo.
Section: abstract
Claim 35perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks light-induced phase advances in vivo.

CRE-decoy ODN also blocked light-induced phase advances in vivo.
Section: abstract
Claim 36requirement for signalingsupports2003Source 1needs review

CREB-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.

Ca2+/cAMP Response Element-binding Protein (CREB)-dependent Activation of Per1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
Section: title/abstract
Claim 37requirement for signalingsupports2003Source 1needs review

CREB-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.

Ca2+/cAMP Response Element-binding Protein (CREB)-dependent Activation of Per1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
Section: title/abstract
Claim 38requirement for signalingsupports2003Source 1needs review

CREB-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.

Ca2+/cAMP Response Element-binding Protein (CREB)-dependent Activation of Per1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
Section: title/abstract
Claim 39requirement for signalingsupports2003Source 1needs review

CREB-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.

Ca2+/cAMP Response Element-binding Protein (CREB)-dependent Activation of Per1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
Section: title/abstract
Claim 40requirement for signalingsupports2003Source 1needs review

CREB-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.

Ca2+/cAMP Response Element-binding Protein (CREB)-dependent Activation of Per1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
Section: title/abstract
Claim 41requirement for signalingsupports2003Source 1needs review

CREB-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.

Ca2+/cAMP Response Element-binding Protein (CREB)-dependent Activation of Per1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
Section: title/abstract
Claim 42requirement for signalingsupports2003Source 1needs review

CREB-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.

Ca2+/cAMP Response Element-binding Protein (CREB)-dependent Activation of Per1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
Section: title/abstract

Approval Evidence

1 source2 linked approval claimsfirst-pass slug antisense-oligodeoxynucleotide-against-per1
Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1

Source:

pathway component rolesupports

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.

Source:

perturbation effectsupports

Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1

Source:

Comparisons

Source-backed strengths

The reported strength is a clear functional phenotype: antisense oligodeoxynucleotide against Per1 blocked glutamate-induced phase advances in the rat SCN. The same study also places Per1 within a CREB-dependent light-signaling pathway, providing pathway-level context for the perturbation result.

Compared with modular light-controlled skeletal muscle-powered bioactuator

antisense oligodeoxynucleotide against Per1 and modular light-controlled skeletal muscle-powered bioactuator address a similar problem space because they share recombination.

Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light

Compared with Opto-Casp8-V2

antisense oligodeoxynucleotide against Per1 and Opto-Casp8-V2 address a similar problem space because they share recombination.

Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light

Compared with pcVP16

antisense oligodeoxynucleotide against Per1 and pcVP16 address a similar problem space because they share recombination.

Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light

Ranked Citations

  1. 1.
    StructuralSource 1Journal of Biological Chemistry2003Claim 1Claim 2Claim 3

    Extracted from this source document.