Source 1primary paper2020Journal of Molecular Biology
Toolkit/blue light-regulated synthetic genetic circuit for CheZ-controlled motility
blue light-regulated synthetic genetic circuit for CheZ-controlled motility
Also known as: blue light-controllable gene circuit, blue light-regulated synthetic genetic circuit
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The blue light-regulated synthetic genetic circuit for CheZ-controlled motility is a synthetic construct pattern developed in programmed Escherichia coli to control bacterial directional motility with blue light. In the cited study, blue light-regulated control of CheZ expression enabled movement away from blue light, consistent with negative phototaxis, and supported aggregation and pattern formation.
Usefulness & Problems
Why this is useful
This construct is useful for externally programming bacterial movement and spatial organization using light as an input. The reported applications include directing aggregation into different patterns and separating two different strains.
Problem solved
The tool addresses the problem of controlling bacterial directional motility in a programmable, light-responsive manner. Specifically, it links blue light input to CheZ expression so that motility behavior and downstream pattern formation can be regulated.
Problem links
enables non-contact spatial control of motility using patterned light
LiteratureIt addresses the limited spatial control of chemotaxis-based motility control by replacing chemical induction with patterned light. This enables non-contact control over where motile bacteria redistribute.
Source:
It addresses the limited spatial control of chemotaxis-based motility control by replacing chemical induction with patterned light. This enables non-contact control over where motile bacteria redistribute.
overcomes limited spatial control of chemotaxis-based motility control
LiteratureIt addresses the limited spatial control of chemotaxis-based motility control by replacing chemical induction with patterned light. This enables non-contact control over where motile bacteria redistribute.
Source:
It addresses the limited spatial control of chemotaxis-based motility control by replacing chemical induction with patterned light. This enables non-contact control over where motile bacteria redistribute.
Published Workflows
Objective: Engineer a blue light-regulated genetic circuit that controls bacterial directional motility with spatial precision and use it to direct migration, aggregation, patterning, and strain separation.
Why it works: The abstract states that light can be delivered to cells in different patterns with precise spatial control, which is used to overcome the limited spatial control of chemotaxis-based induction.
blue-light regulation of CheZ expressionnegative phototaxis-like movement away from blue lightsynthetic genetic circuit designlight-patterned stimulation
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: spectral hardware requirementoperating role: regulator
The available evidence indicates that the circuit operates through blue light-regulated control of CheZ expression in programmed Escherichia coli. However, the supplied material does not specify promoter design, photoreceptor components, plasmid or genomic implementation, or culture and illumination parameters.
The supplied evidence is limited to a single cited study and does not provide quantitative performance metrics, construct architecture, or comparative benchmarking. The evidence also does not describe validation outside programmed E. coli or define operational limits such as light dose response, temporal resolution, or robustness across conditions.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successBacteriaapplication demoEscherichia coli
Inferred from claim c2 during normalization. The circuit enables programmed Escherichia coli cells to increase directional motility and move away from blue light, consistent with negative phototaxis. Derived from claim c2. Quoted text: The circuit developed enables programmed Escherichia coli cells to increase directional motility and move away from the blue light, i.e., that negative phototaxis is utilized.
Source:
successBacteriaapplication demoEscherichia coli
Inferred from claim c3 during normalization. The circuit allows control of cells to form aggregation with different patterns. Derived from claim c3. Quoted text: This further allows the control of the cells to form aggregation with different patterns.
Source:
successBacteriaapplication demo
Inferred from claim c4 during normalization. The circuit can be used to separate two different strains. Derived from claim c4. Quoted text: Further, we showed that the circuit can be used to separate two different strains.
Source:
successBacteriaapplication demoEscherichia coli
Inferred from claim c2 during normalization. The circuit enables programmed Escherichia coli cells to increase directional motility and move away from blue light, consistent with negative phototaxis. Derived from claim c2. Quoted text: The circuit developed enables programmed Escherichia coli cells to increase directional motility and move away from the blue light, i.e., that negative phototaxis is utilized.
Source:
successBacteriaapplication demoEscherichia coli
Inferred from claim c3 during normalization. The circuit allows control of cells to form aggregation with different patterns. Derived from claim c3. Quoted text: This further allows the control of the cells to form aggregation with different patterns.
Source:
successBacteriaapplication demo
Inferred from claim c4 during normalization. The circuit can be used to separate two different strains. Derived from claim c4. Quoted text: Further, we showed that the circuit can be used to separate two different strains.
Source:
successBacteriaapplication demoEscherichia coli
Inferred from claim c2 during normalization. The circuit enables programmed Escherichia coli cells to increase directional motility and move away from blue light, consistent with negative phototaxis. Derived from claim c2. Quoted text: The circuit developed enables programmed Escherichia coli cells to increase directional motility and move away from the blue light, i.e., that negative phototaxis is utilized.
Source:
successBacteriaapplication demoEscherichia coli
Inferred from claim c3 during normalization. The circuit allows control of cells to form aggregation with different patterns. Derived from claim c3. Quoted text: This further allows the control of the cells to form aggregation with different patterns.
Source:
successBacteriaapplication demo
Inferred from claim c4 during normalization. The circuit can be used to separate two different strains. Derived from claim c4. Quoted text: Further, we showed that the circuit can be used to separate two different strains.
Source:
successBacteriaapplication demoEscherichia coli
Inferred from claim c2 during normalization. The circuit enables programmed Escherichia coli cells to increase directional motility and move away from blue light, consistent with negative phototaxis. Derived from claim c2. Quoted text: The circuit developed enables programmed Escherichia coli cells to increase directional motility and move away from the blue light, i.e., that negative phototaxis is utilized.
Source:
successBacteriaapplication demoEscherichia coli
Inferred from claim c3 during normalization. The circuit allows control of cells to form aggregation with different patterns. Derived from claim c3. Quoted text: This further allows the control of the cells to form aggregation with different patterns.
Source:
successBacteriaapplication demo
Inferred from claim c4 during normalization. The circuit can be used to separate two different strains. Derived from claim c4. Quoted text: Further, we showed that the circuit can be used to separate two different strains.
Source:
successBacteriaapplication demoEscherichia coli
Inferred from claim c2 during normalization. The circuit enables programmed Escherichia coli cells to increase directional motility and move away from blue light, consistent with negative phototaxis. Derived from claim c2. Quoted text: The circuit developed enables programmed Escherichia coli cells to increase directional motility and move away from the blue light, i.e., that negative phototaxis is utilized.
Source:
successBacteriaapplication demoEscherichia coli
Inferred from claim c3 during normalization. The circuit allows control of cells to form aggregation with different patterns. Derived from claim c3. Quoted text: This further allows the control of the cells to form aggregation with different patterns.
Source:
successBacteriaapplication demo
Inferred from claim c4 during normalization. The circuit can be used to separate two different strains. Derived from claim c4. Quoted text: Further, we showed that the circuit can be used to separate two different strains.
Source:
Supporting Sources
Ranked Claims
The circuit allows control of cells to form aggregation with different patterns.
This further allows the control of the cells to form aggregation with different patterns.
The circuit allows control of cells to form aggregation with different patterns.
This further allows the control of the cells to form aggregation with different patterns.
The circuit allows control of cells to form aggregation with different patterns.
This further allows the control of the cells to form aggregation with different patterns.
The circuit allows control of cells to form aggregation with different patterns.
This further allows the control of the cells to form aggregation with different patterns.
The circuit allows control of cells to form aggregation with different patterns.
This further allows the control of the cells to form aggregation with different patterns.
The circuit can be used to separate two different strains.
Further, we showed that the circuit can be used to separate two different strains.
The circuit can be used to separate two different strains.
Further, we showed that the circuit can be used to separate two different strains.
The circuit can be used to separate two different strains.
Further, we showed that the circuit can be used to separate two different strains.
The circuit can be used to separate two different strains.
Further, we showed that the circuit can be used to separate two different strains.
The circuit can be used to separate two different strains.
Further, we showed that the circuit can be used to separate two different strains.
The circuit enables programmed Escherichia coli cells to increase directional motility and move away from blue light, consistent with negative phototaxis.
The circuit developed enables programmed Escherichia coli cells to increase directional motility and move away from the blue light, i.e., that negative phototaxis is utilized.
The circuit enables programmed Escherichia coli cells to increase directional motility and move away from blue light, consistent with negative phototaxis.
The circuit developed enables programmed Escherichia coli cells to increase directional motility and move away from the blue light, i.e., that negative phototaxis is utilized.
The circuit enables programmed Escherichia coli cells to increase directional motility and move away from blue light, consistent with negative phototaxis.
The circuit developed enables programmed Escherichia coli cells to increase directional motility and move away from the blue light, i.e., that negative phototaxis is utilized.
The circuit enables programmed Escherichia coli cells to increase directional motility and move away from blue light, consistent with negative phototaxis.
The circuit developed enables programmed Escherichia coli cells to increase directional motility and move away from the blue light, i.e., that negative phototaxis is utilized.
The circuit enables programmed Escherichia coli cells to increase directional motility and move away from blue light, consistent with negative phototaxis.
The circuit developed enables programmed Escherichia coli cells to increase directional motility and move away from the blue light, i.e., that negative phototaxis is utilized.
Blue light-controllable gene circuits can regulate CheZ expression to control bacterial motility and pattern formation.
The demonstrated ability of blue light-controllable gene circuits to regulate a CheZ expression could give researchers more means to control bacterial motility and pattern formation.
Blue light-controllable gene circuits can regulate CheZ expression to control bacterial motility and pattern formation.
The demonstrated ability of blue light-controllable gene circuits to regulate a CheZ expression could give researchers more means to control bacterial motility and pattern formation.
Blue light-controllable gene circuits can regulate CheZ expression to control bacterial motility and pattern formation.
The demonstrated ability of blue light-controllable gene circuits to regulate a CheZ expression could give researchers more means to control bacterial motility and pattern formation.
Blue light-controllable gene circuits can regulate CheZ expression to control bacterial motility and pattern formation.
The demonstrated ability of blue light-controllable gene circuits to regulate a CheZ expression could give researchers more means to control bacterial motility and pattern formation.
Blue light-controllable gene circuits can regulate CheZ expression to control bacterial motility and pattern formation.
The demonstrated ability of blue light-controllable gene circuits to regulate a CheZ expression could give researchers more means to control bacterial motility and pattern formation.
A blue light-regulated synthetic genetic circuit was developed to control bacterial directional motility.
we developed blue light-regulated synthetic genetic circuit to control bacterial directional motility
A blue light-regulated synthetic genetic circuit was developed to control bacterial directional motility.
we developed blue light-regulated synthetic genetic circuit to control bacterial directional motility
A blue light-regulated synthetic genetic circuit was developed to control bacterial directional motility.
we developed blue light-regulated synthetic genetic circuit to control bacterial directional motility
A blue light-regulated synthetic genetic circuit was developed to control bacterial directional motility.
we developed blue light-regulated synthetic genetic circuit to control bacterial directional motility
A blue light-regulated synthetic genetic circuit was developed to control bacterial directional motility.
we developed blue light-regulated synthetic genetic circuit to control bacterial directional motility
Approval Evidence
1 source5 linked approval claimsfirst-pass slug blue-light-regulated-synthetic-genetic-circuit-for-chez-controlled-motility
we developed blue light-regulated synthetic genetic circuit to control bacterial directional motility... The demonstrated ability of blue light-controllable gene circuits to regulate a CheZ expression
Source:
application effectsupports
The circuit allows control of cells to form aggregation with different patterns.
This further allows the control of the cells to form aggregation with different patterns.
Source:
application effectsupports
The circuit can be used to separate two different strains.
Further, we showed that the circuit can be used to separate two different strains.
Source:
behavioral effectsupports
The circuit enables programmed Escherichia coli cells to increase directional motility and move away from blue light, consistent with negative phototaxis.
The circuit developed enables programmed Escherichia coli cells to increase directional motility and move away from the blue light, i.e., that negative phototaxis is utilized.
Source:
mechanismsupports
Blue light-controllable gene circuits can regulate CheZ expression to control bacterial motility and pattern formation.
The demonstrated ability of blue light-controllable gene circuits to regulate a CheZ expression could give researchers more means to control bacterial motility and pattern formation.
Source:
tool capabilitysupports
A blue light-regulated synthetic genetic circuit was developed to control bacterial directional motility.
we developed blue light-regulated synthetic genetic circuit to control bacterial directional motility
Source:
Comparisons
Source-stated alternatives
The abstract contrasts this approach with chemotaxis using prescribed chemical stimuli that require physical contact with the inducer. No other alternative platform is described in the primary abstract itself.
Source:
The abstract contrasts this approach with chemotaxis using prescribed chemical stimuli that require physical contact with the inducer. No other alternative platform is described in the primary abstract itself.
Source-backed strengths
The reported system was developed to control bacterial directional motility and produced a behavioral output in which programmed E. coli increased directional motility and moved away from blue light. It also supported higher-order spatial outcomes, including aggregation with different patterns and separation of two strains.
blue light-regulated synthetic genetic circuit for CheZ-controlled motility and modular light-controlled skeletal muscle-powered bioactuator address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Strengths here: looks easier to implement in practice.
Compared with Opto-Casp8-V2
blue light-regulated synthetic genetic circuit for CheZ-controlled motility and Opto-Casp8-V2 address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Strengths here: looks easier to implement in practice.
Compared with pcVP16
blue light-regulated synthetic genetic circuit for CheZ-controlled motility and pcVP16 address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.