Source 1primary paper2021Advanced Biology
Toolkit/blue light-responsive Cas13b mRNA knockdown system
blue light-responsive Cas13b mRNA knockdown system
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The blue light-responsive Cas13b mRNA knockdown system is an optogenetic RNA-control platform in which blue light induces expression of an engineered Cas13b that specifically degrades target mRNAs. In the reported application, combining three blue light-inducible switches reduced protein levels by more than 99%.
Usefulness & Problems
Why this is useful
This system is useful for light-triggered, sequence-specific post-transcriptional suppression of gene expression through targeted mRNA degradation. It addresses the need for optically controlled RNA knockdown using an engineered Cas13b effector rather than constitutive knockdown.
Source:
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
Problem solved
It helps solve the problem of achieving inducible and specific mRNA knockdown with blue light as the input signal. The reported design links blue light-inducible gene expression to Cas13b-mediated target mRNA degradation.
Source:
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
Problem links
Need conditional protein clearance
DerivedThe blue light-responsive Cas13b mRNA knockdown system is an optogenetic RNA-control platform in which blue light induces expression of an engineered Cas13b that specifically degrades target mRNAs. In the reported application, combining three blue light-inducible switches reduced protein levels by more than 99%.
Need precise spatiotemporal control with light input
DerivedThe blue light-responsive Cas13b mRNA knockdown system is an optogenetic RNA-control platform in which blue light induces expression of an engineered Cas13b that specifically degrades target mRNAs. In the reported application, combining three blue light-inducible switches reduced protein levels by more than 99%.
Need tighter control over gene expression timing or amplitude
DerivedThe blue light-responsive Cas13b mRNA knockdown system is an optogenetic RNA-control platform in which blue light induces expression of an engineered Cas13b that specifically degrades target mRNAs. In the reported application, combining three blue light-inducible switches reduced protein levels by more than 99%.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level RNA part used inside a larger architecture that realizes a mechanism.
Mechanisms
DegradationDegradationlight-inducible gene expressionlight-inducible gene expressionrna-guided target mrna degradationrna-guided target mrna degradationTechniques
No technique tags yet.
Target processes
degradationtranscriptionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulator
The system is described as relying on blue light-inducible expression of an engineered Cas13b, which then degrades target mRNAs specifically. The provided evidence does not specify the photoreceptor module, construct architecture, guide design, host system, or delivery method.
The supplied evidence does not provide details on target range, kinetics, reversibility, background activity in the dark, or performance across cell types and organisms. Independent replication and broad validation are not documented in the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
protein level reduction 99 %
Approval Evidence
1 source1 linked approval claimfirst-pass slug blue-light-responsive-cas13b-mrna-knockdown-system
the Zurbriggen group describes a blue light-responsive system for mRNA knockdown. The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically.
Source:
application resultsupports
A blue light-responsive engineered Cas13b system enables specific target mRNA degradation, and combining three blue light-inducible switches can reduce protein levels by more than 99%.
The system is based on the blue light-inducible expression of an engineered Cas13b to degrade target mRNAs specifically. By combining three blue light-inducible switches for mRNA degradation, suppressing transcription, and target protein destabilization, the protein levels can rapidly be reduced by >99%.
Source:
Comparisons
Source-backed strengths
The reported strength is specific target mRNA degradation mediated by an engineered Cas13b under blue light control. In the cited application, combining three blue light-inducible switches reduced protein levels by more than 99%, indicating strong functional suppression in that configuration.
Compared with CRY1
blue light-responsive Cas13b mRNA knockdown system and CRY1 address a similar problem space because they share degradation, transcription.
Shared frame: shared target processes: degradation, transcription; shared mechanisms: degradation; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Compared with Cry2
blue light-responsive Cas13b mRNA knockdown system and Cry2 address a similar problem space because they share degradation, transcription.
Shared frame: shared target processes: degradation, transcription; shared mechanisms: degradation; same primary input modality: light
Strengths here: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Relative tradeoffs: appears more independently replicated.
blue light-responsive Cas13b mRNA knockdown system and CRY2 and LOV-fused degron light-responsive repression/degradation system address a similar problem space because they share degradation, transcription.
Shared frame: shared target processes: degradation, transcription; shared mechanisms: degradation; same primary input modality: light
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.