Source 1primary paper2024MED
Toolkit/lyso-ArchT
lyso-ArchT
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Here, we constructed lysosome-localized optogenetic actuators, named lyso-NpHR3.0, lyso-ArchT, and lyso-ChR2, to achieve optogenetic manipulation of lysosomes.
Usefulness & Problems
Why this is useful
lyso-ArchT is a lysosome-localized optogenetic actuator constructed to manipulate lysosomal physiology with light. The abstract groups it with tools that control lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics.; optogenetic manipulation of lysosomes; light-dependent control of lysosomal physiology in living cells
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lyso-ArchT is a lysosome-localized optogenetic actuator constructed to manipulate lysosomal physiology with light. The abstract groups it with tools that control lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics.
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optogenetic manipulation of lysosomes
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light-dependent control of lysosomal physiology in living cells
Problem solved
It addresses the lack of methods to dynamically regulate lysosomal function in living cells or animals.; lack of methods to dynamically regulate lysosomal function in living cells or animals
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It addresses the lack of methods to dynamically regulate lysosomal function in living cells or animals.
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lack of methods to dynamically regulate lysosomal function in living cells or animals
Problem links
lack of methods to dynamically regulate lysosomal function in living cells or animals
LiteratureIt addresses the lack of methods to dynamically regulate lysosomal function in living cells or animals.
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It addresses the lack of methods to dynamically regulate lysosomal function in living cells or animals.
Published Workflows
Optogenetic manipulation of lysosomal physiology and autophagy-dependent clearance of amyloid beta.
2024Objective: Construct lysosome-localized optogenetic actuators to dynamically regulate lysosomal physiology and test whether lysosomal optical control can drive autophagy and amyloid beta clearance in living cells and animals.
Why it works: The paper's logic is that lysosome-localized optogenetic actuators allow dynamic, light-dependent perturbation of lysosomal physiological parameters, enabling causal testing of how lysosomal state affects autophagy and amyloid beta clearance.
optical control of lysosomal physiologyautophagy induction through the mTOR pathwayautophagy-dependent Aβ clearanceconstruction of lysosome-localized optogenetic actuatorslight-dependent perturbation in living cells and animals
Stages
- 1.Construction of lysosome-localized optogenetic actuators(library_build)
This stage creates the engineered tools needed for dynamic optical perturbation of lysosomal function.
Selection: Build lysosome-localized actuators lyso-NpHR3.0, lyso-ArchT, and lyso-ChR2 for optogenetic manipulation of lysosomes.
- 2.Functional characterization of lysosomal physiological control in living cells(functional_characterization)
This stage establishes that the engineered actuators actually modulate lysosomal physiology in living cells before disease-relevant applications are tested.
Selection: Test whether the new actuators enable light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics in living cells.
- 3.Application testing of lyso-ChR2 in autophagy and amyloid beta clearance(confirmatory_validation)
This stage tests whether lysosomal optical control translates into mechanistic and disease-relevant cellular outcomes.
Selection: Use lyso-ChR2 to test autophagy induction through the mTOR pathway and autophagy-dependent Aβ clearance in cellular models.
- 4.In vivo validation in a Caenorhabditis elegans Alzheimer's disease model(in_vivo_validation)
This stage extends cellular findings to an animal disease model.
Selection: Test whether lyso-ChR2 alleviates Aβ-induced paralysis in a Caenorhabditis elegans model of Alzheimer's disease.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
Degradationlight-dependent modulation of lysosomal ca2+ dynamicslight-dependent modulation of lysosomal degradationlight-dependent modulation of lysosomal hydrolase activitylight-dependent modulation of lysosomal membrane potentiallight-dependent modulation of lysosomal phoptogenetic controlTechniques
No technique tags yet.
Target processes
degradationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulator
Use requires expression of a lysosome-localized optogenetic construct and light stimulation in living cells or animals.; requires light activation; requires lysosome-localized actuator expression
Needs compatible illumination hardware and optical access. Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
lyso-ChR2 activation promotes Aβ clearance in an autophagy-dependent manner in cellular models.
promotes Aβ clearance in an autophagy-dependent manner in cellular models
lyso-ChR2 alleviates Aβ-induced paralysis in the Caenorhabditis elegans model of Alzheimer's disease.
and alleviates Aβ-induced paralysis in the Caenorhabditis elegans model of Alzheimer's disease
lyso-ChR2 activation induces autophagy through the mTOR pathway.
Notably, lyso-ChR2 activation induces autophagy through the mTOR pathway
lyso-NpHR3.0, lyso-ArchT, and lyso-ChR2 are lysosome-localized optogenetic actuators constructed to achieve optogenetic manipulation of lysosomes.
Here, we constructed lysosome-localized optogenetic actuators, named lyso-NpHR3.0, lyso-ArchT, and lyso-ChR2, to achieve optogenetic manipulation of lysosomes.
The lysosome-localized optogenetic actuators enable light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics in living cells.
These new actuators enable light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics in living cells.
Lysosomal optogenetic actuators provide a method to dynamically regulate lysosomal physiology and function in living cells and animals.
Our lysosomal optogenetic actuators supplement the optogenetic toolbox and provide a method to dynamically regulate lysosomal physiology and function in living cells and animals.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug lyso-archt
Here, we constructed lysosome-localized optogenetic actuators, named lyso-NpHR3.0, lyso-ArchT, and lyso-ChR2, to achieve optogenetic manipulation of lysosomes.
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tool capabilitysupports
lyso-NpHR3.0, lyso-ArchT, and lyso-ChR2 are lysosome-localized optogenetic actuators constructed to achieve optogenetic manipulation of lysosomes.
Here, we constructed lysosome-localized optogenetic actuators, named lyso-NpHR3.0, lyso-ArchT, and lyso-ChR2, to achieve optogenetic manipulation of lysosomes.
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tool capabilitysupports
The lysosome-localized optogenetic actuators enable light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics in living cells.
These new actuators enable light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics in living cells.
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tool use casesupports
Lysosomal optogenetic actuators provide a method to dynamically regulate lysosomal physiology and function in living cells and animals.
Our lysosomal optogenetic actuators supplement the optogenetic toolbox and provide a method to dynamically regulate lysosomal physiology and function in living cells and animals.
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Comparisons
Source-stated alternatives
The same paper presents lyso-NpHR3.0 and lyso-ChR2 as related lysosomal optogenetic actuators.
Source:
The same paper presents lyso-NpHR3.0 and lyso-ChR2 as related lysosomal optogenetic actuators.
Source-backed strengths
lysosome-localized optogenetic actuator; part of a tool set enabling light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics
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lysosome-localized optogenetic actuator
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part of a tool set enabling light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics
Compared with lyso-ChR2
The same paper presents lyso-NpHR3.0 and lyso-ChR2 as related lysosomal optogenetic actuators.
Shared frame: source-stated alternative in extracted literature
Strengths here: lysosome-localized optogenetic actuator; part of a tool set enabling light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics.
Source:
The same paper presents lyso-NpHR3.0 and lyso-ChR2 as related lysosomal optogenetic actuators.
Compared with lyso-NpHR3.0
The same paper presents lyso-NpHR3.0 and lyso-ChR2 as related lysosomal optogenetic actuators.
Shared frame: source-stated alternative in extracted literature
Strengths here: lysosome-localized optogenetic actuator; part of a tool set enabling light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics.
Source:
The same paper presents lyso-NpHR3.0 and lyso-ChR2 as related lysosomal optogenetic actuators.
Compared with optogenetic
The same paper presents lyso-NpHR3.0 and lyso-ChR2 as related lysosomal optogenetic actuators.
Shared frame: source-stated alternative in extracted literature
Strengths here: lysosome-localized optogenetic actuator; part of a tool set enabling light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics.
Source:
The same paper presents lyso-NpHR3.0 and lyso-ChR2 as related lysosomal optogenetic actuators.
Compared with optogenetic actuator
The same paper presents lyso-NpHR3.0 and lyso-ChR2 as related lysosomal optogenetic actuators.
Shared frame: source-stated alternative in extracted literature
Strengths here: lysosome-localized optogenetic actuator; part of a tool set enabling light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics.
Source:
The same paper presents lyso-NpHR3.0 and lyso-ChR2 as related lysosomal optogenetic actuators.
Compared with optogenetic actuators
The same paper presents lyso-NpHR3.0 and lyso-ChR2 as related lysosomal optogenetic actuators.
Shared frame: source-stated alternative in extracted literature
Strengths here: lysosome-localized optogenetic actuator; part of a tool set enabling light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics.
Source:
The same paper presents lyso-NpHR3.0 and lyso-ChR2 as related lysosomal optogenetic actuators.
Ranked Citations
- 1.