Source 1primary paper1998Nature Medicine
Toolkit/BrdU immunofluorescent labeling with neuronal marker co-labeling
BrdU immunofluorescent labeling with neuronal marker co-labeling
Also known as: BrdU labeling, immunofluorescent labeling for BrdU and neuronal markers
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Human brain tissue was obtained postmortem from patients who had been treated with the thymidine analog, bromodeoxyuridine (BrdU), that labels DNA during the S phase. Using immunofluorescent labeling for BrdU and for one of the neuronal markers, NeuN, calbindin or neuron specific enolase (NSE), we demonstrate that new neurons ... are generated from dividing progenitor cells in the dentate gyrus of adult humans.
Usefulness & Problems
Why this is useful
BrdU labeling is presented in the supplied research summary as a method used in foundational rat and human studies to label dividing cells relevant to adult neurogenesis.; labeling dividing cells in adult neurogenesis studies; supporting demonstration of newly generated cells in brain tissue; This assay labels cells that incorporated BrdU during S phase and tests whether those labeled cells also express neuronal markers. In this paper it is used to identify newly generated neurons in adult human dentate gyrus.; identifying cells that underwent DNA synthesis and assessing whether labeled cells express neuronal markers in adult human hippocampal tissue
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BrdU labeling is presented in the supplied research summary as a method used in foundational rat and human studies to label dividing cells relevant to adult neurogenesis.
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labeling dividing cells in adult neurogenesis studies
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supporting demonstration of newly generated cells in brain tissue
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This assay labels cells that incorporated BrdU during S phase and tests whether those labeled cells also express neuronal markers. In this paper it is used to identify newly generated neurons in adult human dentate gyrus.
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identifying cells that underwent DNA synthesis and assessing whether labeled cells express neuronal markers in adult human hippocampal tissue
Problem solved
It helps provide technical evidence for newly generated cells, addressing the review's emphasis on the need for unequivocal demonstration and quantification.; addresses the need for technical strategies to demonstrate and quantify newly generated cells; It addresses the problem of demonstrating whether dividing cells in the adult human hippocampus can give rise to cells with neuronal marker expression.; provides a way to link prior cell division labeling to neuronal identity in postmortem human tissue
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It helps provide technical evidence for newly generated cells, addressing the review's emphasis on the need for unequivocal demonstration and quantification.
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addresses the need for technical strategies to demonstrate and quantify newly generated cells
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It addresses the problem of demonstrating whether dividing cells in the adult human hippocampus can give rise to cells with neuronal marker expression.
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provides a way to link prior cell division labeling to neuronal identity in postmortem human tissue
Problem links
addresses the need for technical strategies to demonstrate and quantify newly generated cells
LiteratureIt helps provide technical evidence for newly generated cells, addressing the review's emphasis on the need for unequivocal demonstration and quantification.
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It helps provide technical evidence for newly generated cells, addressing the review's emphasis on the need for unequivocal demonstration and quantification.
provides a way to link prior cell division labeling to neuronal identity in postmortem human tissue
LiteratureIt addresses the problem of demonstrating whether dividing cells in the adult human hippocampus can give rise to cells with neuronal marker expression.
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It addresses the problem of demonstrating whether dividing cells in the adult human hippocampus can give rise to cells with neuronal marker expression.
Published Workflows
Objective: Investigate whether neurogenesis occurs in the adult human brain, specifically in the hippocampal dentate gyrus.
Why it works: The workflow is intended to work by identifying cells that incorporated BrdU during DNA synthesis and then testing whether those same cells express neuronal markers in a known neurogenic region.
BrdU incorporation during S phase marks dividing cellsco-expression of neuronal markers is used to define new neuronspostmortem tissue analysisimmunofluorescent co-labeling
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The provided evidence only supports that it is a labeling method used in tissue studies; specific reagents, dosing, and detection steps are not described here.; requires tissue-based labeling and downstream detection workflows not described in the abstract; The approach requires postmortem human brain tissue from patients previously exposed to BrdU and immunofluorescent labeling reagents for BrdU plus neuronal markers such as NeuN, calbindin, or NSE.; postmortem human brain tissue from BrdU-treated patients is required; immunofluorescent detection of BrdU and neuronal markers is required
This packet does not have enough direct evidence from the review to state how well BrdU alone establishes neuronal identity or physiological integration.; the anchor review abstract does not itself describe protocol details or comparative performance; The abstract does not show that this method alone resolves all lineage or functional maturation questions beyond marker-defined neuronal identity.; requires prior in vivo BrdU exposure before postmortem tissue collection; neuronal classification depends on marker co-labeling rather than direct lineage tracing
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
New neurons are generated from dividing progenitor cells in the dentate gyrus of adult humans.
Approval Evidence
2 sources1 linked approval claimfirst-pass slugs brdu-immunofluorescent-labeling-with-neuronal-marker-co-labeling, brdu-labeling
Related component names supported by discovered sources include BrdU labeling.
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Human brain tissue was obtained postmortem from patients who had been treated with the thymidine analog, bromodeoxyuridine (BrdU), that labels DNA during the S phase. Using immunofluorescent labeling for BrdU and for one of the neuronal markers, NeuN, calbindin or neuron specific enolase (NSE), we demonstrate that new neurons ... are generated from dividing progenitor cells in the dentate gyrus of adult humans.
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existencesupports
New neurons are generated from dividing progenitor cells in the dentate gyrus of adult humans.
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Comparisons
Source-stated alternatives
The supplied research summary also names carbon-14 birth dating and marker-based approaches using DCX, PSA-NCAM, NeuN, and calbindin-D28k.; The upstream web summary notes later orthogonal human studies using atmospheric 14C birth dating, but that alternative is not part of this paper.
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The supplied research summary also names carbon-14 birth dating and marker-based approaches using DCX, PSA-NCAM, NeuN, and calbindin-D28k.
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The upstream web summary notes later orthogonal human studies using atmospheric 14C birth dating, but that alternative is not part of this paper.
Source-backed strengths
identified in the supplied research summary as a directly relevant method in foundational studies; combines a proliferation label with neuronal marker co-labeling in human dentate gyrus tissue
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identified in the supplied research summary as a directly relevant method in foundational studies
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combines a proliferation label with neuronal marker co-labeling in human dentate gyrus tissue
Compared with Langendorff perfused heart electrical recordings
BrdU immunofluorescent labeling with neuronal marker co-labeling and Langendorff perfused heart electrical recordings address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Compared with native green gel system
BrdU immunofluorescent labeling with neuronal marker co-labeling and native green gel system address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
BrdU immunofluorescent labeling with neuronal marker co-labeling and sub-picosecond pump-probe analysis of bacteriorhodopsin pigments address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.