Source 1primary paper2016MED
Toolkit/CRISPR-plus
CRISPR-plus
Also known as: CRISPR-precise light-mediated unveiling of sgRNAs
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
CRISPR-plus is a light-activated CRISPR/Cas9 strategy in which guide RNA activity is suppressed by photocleavable protectors and restored by illumination. It enables optical control of genome editing and was reported to be compatible with simultaneous targeting of multiple DNA sequences.
Usefulness & Problems
Why this is useful
This construct pattern is useful for imposing light-dependent control over CRISPR/Cas9 activity through the guide RNA rather than by altering Cas9 itself. The reported compatibility with simultaneous targeting of multiple DNA sequences suggests utility for multiplexed editing designs that require temporal activation by light.
Problem solved
CRISPR-plus addresses the problem of how to keep CRISPR/Cas9 inactive until a defined light stimulus is applied. The method specifically solves this by photocaging guide RNA activity with photocleavable protectors, thereby enabling light-triggered unveiling of sgRNA function.
Problem links
adds remote temporal control to CRISPR/Cas9 activity
LiteratureIt addresses the need to remotely trigger CRISPR/Cas9 activity with greater precision and complexity. It also supports multiplex DNA targeting and guide-RNA labeling applications.
Source:
It addresses the need to remotely trigger CRISPR/Cas9 activity with greater precision and complexity. It also supports multiplex DNA targeting and guide-RNA labeling applications.
enables optical unveiling of otherwise inactive sgRNAs
LiteratureIt addresses the need to remotely trigger CRISPR/Cas9 activity with greater precision and complexity. It also supports multiplex DNA targeting and guide-RNA labeling applications.
Source:
It addresses the need to remotely trigger CRISPR/Cas9 activity with greater precision and complexity. It also supports multiplex DNA targeting and guide-RNA labeling applications.
Published Workflows
Objective: Develop a light-activated CRISPR/Cas9 method by photocaging guide RNA activity so genome targeting can be remotely triggered with greater precision and complexity.
Why it works: The abstract indicates that guide RNA activity is held inactive by photocaging and then unveiled by light, allowing remote triggering of CRISPR/Cas9 function.
photocaging of guide RNA activitylight-mediated unveiling of sgRNAsoptical activationguide RNA engineering
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
editingInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: cleavageswitch architecture: uncaging
Implementation involves guide RNAs bearing photocleavable protectors that block activity until light exposure. The supplied evidence does not provide practical details on protector chemistry, construct architecture, delivery format, or required optical parameters.
The provided evidence does not specify illumination wavelength, uncaging kinetics, editing efficiency, off-target behavior, or performance across cell types or organisms. Independent replication is not documented in the supplied material, so validation breadth remains limited.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
CRISPR-plus photoactivation is compatible with simultaneous targeting of multiple DNA sequences.
The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences
CRISPR-plus photoactivation is compatible with simultaneous targeting of multiple DNA sequences.
The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences
CRISPR-plus photoactivation is compatible with simultaneous targeting of multiple DNA sequences.
The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences
CRISPR-plus photoactivation is compatible with simultaneous targeting of multiple DNA sequences.
The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences
CRISPR-plus photoactivation is compatible with simultaneous targeting of multiple DNA sequences.
The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences
CRISPR-plus photoactivation is compatible with simultaneous targeting of multiple DNA sequences.
The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences
CRISPR-plus photoactivation is compatible with simultaneous targeting of multiple DNA sequences.
The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences
CRISPR-plus photoactivation is compatible with simultaneous targeting of multiple DNA sequences.
The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences
The paper reports development of CRISPR-plus, a method that photocages guide RNA activity to enable light-activated CRISPR/Cas9.
In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs).
The paper reports development of CRISPR-plus, a method that photocages guide RNA activity to enable light-activated CRISPR/Cas9.
In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs).
The paper reports development of CRISPR-plus, a method that photocages guide RNA activity to enable light-activated CRISPR/Cas9.
In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs).
The paper reports development of CRISPR-plus, a method that photocages guide RNA activity to enable light-activated CRISPR/Cas9.
In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs).
The paper reports development of CRISPR-plus, a method that photocages guide RNA activity to enable light-activated CRISPR/Cas9.
In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs).
The paper reports development of CRISPR-plus, a method that photocages guide RNA activity to enable light-activated CRISPR/Cas9.
In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs).
The paper reports development of CRISPR-plus, a method that photocages guide RNA activity to enable light-activated CRISPR/Cas9.
In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs).
The paper reports development of CRISPR-plus, a method that photocages guide RNA activity to enable light-activated CRISPR/Cas9.
In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs).
CRISPR-plus supports guide RNA modifications that can enable labeling for imaging and mechanistic investigations.
supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations
CRISPR-plus supports guide RNA modifications that can enable labeling for imaging and mechanistic investigations.
supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations
CRISPR-plus supports guide RNA modifications that can enable labeling for imaging and mechanistic investigations.
supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations
CRISPR-plus supports guide RNA modifications that can enable labeling for imaging and mechanistic investigations.
supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations
CRISPR-plus supports guide RNA modifications that can enable labeling for imaging and mechanistic investigations.
supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations
CRISPR-plus supports guide RNA modifications that can enable labeling for imaging and mechanistic investigations.
supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations
CRISPR-plus supports guide RNA modifications that can enable labeling for imaging and mechanistic investigations.
supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations
CRISPR-plus supports guide RNA modifications that can enable labeling for imaging and mechanistic investigations.
supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations
Approval Evidence
1 source3 linked approval claimsfirst-pass slug crispr-plus
we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs).
Source:
compatibilitysupports
CRISPR-plus photoactivation is compatible with simultaneous targeting of multiple DNA sequences.
The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences
Source:
method developmentsupports
The paper reports development of CRISPR-plus, a method that photocages guide RNA activity to enable light-activated CRISPR/Cas9.
In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs).
Source:
modification supportsupports
CRISPR-plus supports guide RNA modifications that can enable labeling for imaging and mechanistic investigations.
supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations
Source:
Comparisons
Source-stated alternatives
Upstream source discovery identifies orthogonal light-activated Cas9 architectures such as paCas9 and ps-Cas9 as nearby alternatives. Those comparators regulate the Cas9 protein rather than the guide RNA layer.
Source:
Upstream source discovery identifies orthogonal light-activated Cas9 architectures such as paCas9 and ps-Cas9 as nearby alternatives. Those comparators regulate the Cas9 protein rather than the guide RNA layer.
Source-backed strengths
The central strength supported by the source is optical activation of CRISPR/Cas9 through photocaged guide RNAs. The source also states that photoactivation is compatible with simultaneous targeting of multiple DNA sequences, indicating support for multiplexed genome editing.
Compared with CRISPR/Cas9
Upstream source discovery identifies orthogonal light-activated Cas9 architectures such as paCas9 and ps-Cas9 as nearby alternatives. Those comparators regulate the Cas9 protein rather than the guide RNA layer.
Shared frame: source-stated alternative in extracted literature
Strengths here: compatible with simultaneous targeting of multiple DNA sequences; supports numerous guide RNA modifications.
Relative tradeoffs: abstract does not report quantitative performance or biological validation context.
Source:
Upstream source discovery identifies orthogonal light-activated Cas9 architectures such as paCas9 and ps-Cas9 as nearby alternatives. Those comparators regulate the Cas9 protein rather than the guide RNA layer.
Compared with CRISPR/Cas9 system
Upstream source discovery identifies orthogonal light-activated Cas9 architectures such as paCas9 and ps-Cas9 as nearby alternatives. Those comparators regulate the Cas9 protein rather than the guide RNA layer.
Shared frame: source-stated alternative in extracted literature
Strengths here: compatible with simultaneous targeting of multiple DNA sequences; supports numerous guide RNA modifications.
Relative tradeoffs: abstract does not report quantitative performance or biological validation context.
Source:
Upstream source discovery identifies orthogonal light-activated Cas9 architectures such as paCas9 and ps-Cas9 as nearby alternatives. Those comparators regulate the Cas9 protein rather than the guide RNA layer.
Compared with guide RNA
Upstream source discovery identifies orthogonal light-activated Cas9 architectures such as paCas9 and ps-Cas9 as nearby alternatives. Those comparators regulate the Cas9 protein rather than the guide RNA layer.
Shared frame: source-stated alternative in extracted literature
Strengths here: compatible with simultaneous targeting of multiple DNA sequences; supports numerous guide RNA modifications.
Relative tradeoffs: abstract does not report quantitative performance or biological validation context.
Source:
Upstream source discovery identifies orthogonal light-activated Cas9 architectures such as paCas9 and ps-Cas9 as nearby alternatives. Those comparators regulate the Cas9 protein rather than the guide RNA layer.
Compared with synthetically engineered guide RNA
Upstream source discovery identifies orthogonal light-activated Cas9 architectures such as paCas9 and ps-Cas9 as nearby alternatives. Those comparators regulate the Cas9 protein rather than the guide RNA layer.
Shared frame: source-stated alternative in extracted literature
Strengths here: compatible with simultaneous targeting of multiple DNA sequences; supports numerous guide RNA modifications.
Relative tradeoffs: abstract does not report quantitative performance or biological validation context.
Source:
Upstream source discovery identifies orthogonal light-activated Cas9 architectures such as paCas9 and ps-Cas9 as nearby alternatives. Those comparators regulate the Cas9 protein rather than the guide RNA layer.
Ranked Citations
- 1.