Source 1primary paper2020Physical Review Letters
Toolkit/domain mobility measurement
domain mobility measurement
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Measurements of domain mobility indicated the presence of 120 nm wide ordered and 40 nm wide disordered domains.
Usefulness & Problems
Why this is useful
The method uses domain mobility measurements to infer the presence and size of ordered and disordered membrane domains.; inferring membrane domain size from mobility measurements
Source:
The method uses domain mobility measurements to infer the presence and size of ordered and disordered membrane domains.
Source:
inferring membrane domain size from mobility measurements
Problem solved
It helps estimate nanoscale domain sizes when direct light-microscopy observation is limited by small feature size.; provides an indirect way to estimate sizes of domains too small for direct light-microscopy observation
Source:
It helps estimate nanoscale domain sizes when direct light-microscopy observation is limited by small feature size.
Source:
provides an indirect way to estimate sizes of domains too small for direct light-microscopy observation
Problem links
provides an indirect way to estimate sizes of domains too small for direct light-microscopy observation
LiteratureIt helps estimate nanoscale domain sizes when direct light-microscopy observation is limited by small feature size.
Source:
It helps estimate nanoscale domain sizes when direct light-microscopy observation is limited by small feature size.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensorswitch architecture: recruitment
It requires a membrane system in which domain mobility can be measured; the abstract does not specify the exact instrumentation or model used.; requires measurable domain mobility in the membrane system
It does not directly image the full domain structure at the inferred nanometer scale in the abstract.; domain sizes are inferred from mobility rather than directly resolved by light microscopy
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Photoswitchable lipids dissolved or reformed membrane domains.
Photoswitchable lipids dissolved or reformed the domains.
Differently colored dyes were used as reporters for registration of ordered and disordered lipids from both leaflets of a freestanding bilayer.
Here we used differently colored dyes as reporters for the registration of both ordered and disordered lipids from the two leaves of a freestanding bilayer.
Domain mobility measurements indicated ordered domains of about 120 nm width and disordered domains of about 40 nm width.
Measurements of domain mobility indicated the presence of 120 nm wide ordered and 40 nm wide disordered domains.
disordered domain width 40 nmordered domain width 120 nm
The inferred domain sizes are consistent with predicted roles of line tension and membrane undulation as driving forces for alignment.
These sizes are in line with the predicted roles of line tension and membrane undulation as driving forces for alignment.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug domain-mobility-measurement
Measurements of domain mobility indicated the presence of 120 nm wide ordered and 40 nm wide disordered domains.
Source:
measurement resultsupports
Domain mobility measurements indicated ordered domains of about 120 nm width and disordered domains of about 40 nm width.
Measurements of domain mobility indicated the presence of 120 nm wide ordered and 40 nm wide disordered domains.
Source:
mechanistic interpretationsupports
The inferred domain sizes are consistent with predicted roles of line tension and membrane undulation as driving forces for alignment.
These sizes are in line with the predicted roles of line tension and membrane undulation as driving forces for alignment.
Source:
Comparisons
Source-backed strengths
enabled inference of ordered and disordered domain widths from mobility data
Source:
enabled inference of ordered and disordered domain widths from mobility data
Compared with Langendorff perfused heart electrical recordings
domain mobility measurement and Langendorff perfused heart electrical recordings address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Compared with native green gel system
domain mobility measurement and native green gel system address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
domain mobility measurement and sub-picosecond pump-probe analysis of bacteriorhodopsin pigments address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.