Source 1primary paper2023Biomolecules
Toolkit/dual transient visible absorption/FSRS set-up
dual transient visible absorption/FSRS set-up
Also known as: dual transient visible absorption (visTA)/FSRS set-up, visTA/FSRS
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The dual transient visible absorption (visTA)/FSRS set-up is a broadband time-resolved spectroscopic assay that combines transient visible absorption with femtosecond stimulated Raman spectroscopy. It covers approximately 200–2200 cm^-1 and supports pump–probe delays from a few femtoseconds to several hundreds of microseconds after actinic light excitation, enabling monitoring of photoinduced dynamics.
Usefulness & Problems
Why this is useful
This assay is useful for tracking complete excited-state dynamics across ultrafast to sub-millisecond timescales while simultaneously accessing vibrational and visible transient signals. In the cited application, the extended time scale and wavenumber range enabled monitoring of free FMN in solution and FMN embedded in two EL222 variants.
Source:
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
Problem solved
It addresses the need to follow photoinduced intermediates and dynamics over a wide temporal window with broad spectral coverage in a single experimental set-up. The reported study used it to resolve singlet, triplet, and adduct states and to detect additional dynamical events in the low-frequency Raman region below 1000 cm^-1.
Problem links
Need precise spatiotemporal control with light input
DerivedThe dual transient visible absorption (visTA)/FSRS set-up is a broadband time-resolved spectroscopic assay that combines transient visible absorption with femtosecond stimulated Raman spectroscopy. It spans approximately 200–2200 cm^-1 and supports pump–probe delays from a few femtoseconds to several hundreds of microseconds after actinic light excitation, enabling tracking of photoinduced dynamics.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
Conformational Uncagingstimulated raman scatteringstimulated raman scatteringtransient visible absorptiontransient visible absorptionTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: sensorswitch architecture: multi componentswitch architecture: uncaging
The method uses actinic light excitation followed by pump–probe measurements combining transient visible absorption and femtosecond stimulated Raman spectroscopy. The cited implementation was applied to flavin mononucleotide (FMN) in solution and within EL222 variants, but the supplied evidence does not specify instrument architecture, laser wavelengths, or sample preparation details.
The supplied evidence documents application to FMN free in solution and FMN embedded in two EL222 variants, but does not establish performance across a broader range of chromophores or protein systems. Practical details such as sensitivity limits, sample requirements, and throughput are not provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
low frequency region upper bound 1000 cm^-1
Observed lifetimes and intermediate states including singlet, triplet, and adduct agree with previous time-resolved infrared spectroscopy experiments.
The observed lifetimes and intermediate states (singlet, triplet, and adduct) are in agreement with previous time-resolved infrared spectroscopy experiments.
Observed lifetimes and intermediate states including singlet, triplet, and adduct agree with previous time-resolved infrared spectroscopy experiments.
The observed lifetimes and intermediate states (singlet, triplet, and adduct) are in agreement with previous time-resolved infrared spectroscopy experiments.
Observed lifetimes and intermediate states including singlet, triplet, and adduct agree with previous time-resolved infrared spectroscopy experiments.
The observed lifetimes and intermediate states (singlet, triplet, and adduct) are in agreement with previous time-resolved infrared spectroscopy experiments.
Observed lifetimes and intermediate states including singlet, triplet, and adduct agree with previous time-resolved infrared spectroscopy experiments.
The observed lifetimes and intermediate states (singlet, triplet, and adduct) are in agreement with previous time-resolved infrared spectroscopy experiments.
Observed lifetimes and intermediate states including singlet, triplet, and adduct agree with previous time-resolved infrared spectroscopy experiments.
The observed lifetimes and intermediate states (singlet, triplet, and adduct) are in agreement with previous time-resolved infrared spectroscopy experiments.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
time delay range a few femtoseconds to several hundreds of microsecondswavenumber range ~200-2200 cm^-1
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug dual-transient-visible-absorption-fsrs-set-up
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
Source:
additional dynamics detectedsupports
Analysis of the low-frequency Raman region below 1000 cm^-1 provided evidence for additional dynamical events.
Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm<sup>-1</sup>.
Source:
method applicationsupports
The extended time scale and wavenumber range allowed monitoring of the complete excited-state dynamics of FMN free in solution and FMN embedded in two EL222 variants.
The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222.
Source:
method capabilitysupports
A broadband dual visTA/FSRS set-up spans approximately 200-2200 cm^-1 and supports delays from a few femtoseconds to several hundreds of microseconds after actinic pumping.
Here we report on a broadband (~200-2200 cm<sup>-1</sup>) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump.
Source:
method utilitysupports
Fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Source:
Comparisons
Source-backed strengths
The set-up provides broadband Raman coverage (~200–2200 cm^-1) and time delays spanning from a few femtoseconds to several hundreds of microseconds after actinic pumping. In the reported FMN/EL222 study, observed lifetimes and intermediate states agreed with prior time-resolved infrared spectroscopy, and low-frequency Raman analysis provided evidence for additional dynamical events.
Compared with hydrogen-deuterium exchange coupled to mass spectrometry
dual transient visible absorption/FSRS set-up and hydrogen-deuterium exchange coupled to mass spectrometry address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational_uncaging; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice.
Compared with small-angle X-ray scattering
dual transient visible absorption/FSRS set-up and small-angle X-ray scattering address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational_uncaging; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice.
Compared with temperature-dependent FTIR spectroscopy
dual transient visible absorption/FSRS set-up and temperature-dependent FTIR spectroscopy address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational_uncaging; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice.
Ranked Citations
- 1.