Source 1primary paper2009Biophysical Journal
Toolkit/temperature-dependent FTIR spectroscopy
temperature-dependent FTIR spectroscopy
Also known as: FTIR spectroscopy
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Temperature-dependent FTIR spectroscopy is a structural characterization assay used to record conformational heterogeneity and the propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1. In the cited work, it functions as an infrared spectroscopic method for analyzing a light-responsive protein domain.
Usefulness & Problems
Why this is useful
This assay is useful for probing structural changes in the LOV2/Jα photosensory module as a function of temperature. The available evidence supports its use for characterizing conformational heterogeneity in a light-responsive protein domain.
Problem solved
It addresses the problem of detecting and describing conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1. The evidence does not provide broader problem framing beyond this specific structural analysis application.
Problem links
Need precise spatiotemporal control with light input
DerivedTemperature-dependent FTIR spectroscopy is an assay method used to study conformational heterogeneity and the propagation of structural changes in the LOV2/Jα domain of Avena sativa phototropin 1. In the cited work, it serves as a structural characterization approach for a light-responsive protein domain.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensorswitch architecture: uncaging
The available evidence identifies the assay as temperature-dependent FTIR spectroscopy applied to the LOV2/Jα domain of Avena sativa phototropin 1. No additional practical details on instrumentation, sample preparation, cofactors, construct design, or expression system are provided in the supplied material.
The supplied evidence is limited to a single study on the LOV2/Jα domain from Avena sativa phototropin 1. No performance metrics, spectral resolution details, sample requirements, or validation in other proteins or systems are provided.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
Approval Evidence
1 source1 linked approval claimfirst-pass slug temperature-dependent-ftir-spectroscopy
as Recorded by Temperature-Dependent FTIR Spectroscopy
Source:
structural characterizationsupports
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
Source:
Comparisons
Source-backed strengths
The cited study specifically used temperature-dependent FTIR spectroscopy to record conformational heterogeneity and structural change propagation in the LOV2/Jα domain. This indicates utility for structural characterization of protein conformational responses in this photoreceptor domain.
Compared with atomic force sensing technique
temperature-dependent FTIR spectroscopy and atomic force sensing technique address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational uncaging, conformational_uncaging; same primary input modality: light
Compared with hydrogen-deuterium exchange coupled to mass spectrometry
temperature-dependent FTIR spectroscopy and hydrogen-deuterium exchange coupled to mass spectrometry address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational_uncaging; same primary input modality: light
Compared with small-angle X-ray scattering
temperature-dependent FTIR spectroscopy and small-angle X-ray scattering address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational uncaging, conformational_uncaging; same primary input modality: light
Ranked Citations
- 1.